Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)
We studied 138 glycopeptide-resistant enterococci (GRE) strains, consisting of 131 glycopeptide-resistant Enterococcus faecium (GREfm) and 7 glycopeptide-resistant Enterococcus faecalis (GREfs). The GREfm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the GREfs...
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| Format: | Article |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/150464 |
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| author | M. Biendo C. Adjidé S. Castelain M. Belmekki F. Rousseau M. Slama O. Ganry J. L. Schmit F. Eb |
| author_facet | M. Biendo C. Adjidé S. Castelain M. Belmekki F. Rousseau M. Slama O. Ganry J. L. Schmit F. Eb |
| author_sort | M. Biendo |
| collection | DOAJ |
| description | We studied 138 glycopeptide-resistant enterococci (GRE) strains, consisting of 131 glycopeptide-resistant Enterococcus faecium (GREfm) and 7 glycopeptide-resistant Enterococcus faecalis (GREfs). The GREfm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the GREfs strains were only resistant to vancomycin and teicoplanin. The van A gene was the only glycopeptide determinant present in all GRE isolates investigated. Genes coding for Hyl and Hyl+ Esp were detected in 39 (29.8%) and 92 (70.2%) of the 131 GREfm isolates, respectively. Three of the 7 GREfs were positive for gelE+asa 1 genes, 3 for gel E gene, and 1 for asa 1 gene. The genetic relationship between the 138 GRE was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). GREfm isolates were clustered in a single genogroup (pulsotype A), and GREfs were clustered in six genogroups (pulsotypes B-G). Among the isolates investigated by MLST, only 18 PCR products were sequenced (12 E. faecium and 6 E. faecalis), and 9 sequence types (STs) were identified. |
| format | Article |
| id | doaj-art-2ec0023704214fd0989fcbad4e476f90 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-2ec0023704214fd0989fcbad4e476f902025-08-20T03:55:07ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/150464150464Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)M. Biendo0C. Adjidé1S. Castelain2M. Belmekki3F. Rousseau4M. Slama5O. Ganry6J. L. Schmit7F. Eb8Service de Bactériologie, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceService d'Epidémiologie, Hygiène Hospitalière et Santé Publique, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceUnité de Virologie clinique et fondamentale, Faculté de Médecine et de Pharmacie, 3 rue des Louvels, 80036 Amiens, Cedex, FranceService d'Epidémiologie, Hygiène Hospitalière et Santé Publique, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceService de Bactériologie, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceService de Réanimation Néphrologique, CHU Sud, avenue René Laënnec, 80054 Amiens Cedex 1, FranceService d'Epidémiologie, Hygiène Hospitalière et Santé Publique, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceService de Pathologie Infectieuse, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceService de Bactériologie, CHU Nord, Place Victor Pauchet, 80054 Amiens Cedex 1, FranceWe studied 138 glycopeptide-resistant enterococci (GRE) strains, consisting of 131 glycopeptide-resistant Enterococcus faecium (GREfm) and 7 glycopeptide-resistant Enterococcus faecalis (GREfs). The GREfm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the GREfs strains were only resistant to vancomycin and teicoplanin. The van A gene was the only glycopeptide determinant present in all GRE isolates investigated. Genes coding for Hyl and Hyl+ Esp were detected in 39 (29.8%) and 92 (70.2%) of the 131 GREfm isolates, respectively. Three of the 7 GREfs were positive for gelE+asa 1 genes, 3 for gel E gene, and 1 for asa 1 gene. The genetic relationship between the 138 GRE was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). GREfm isolates were clustered in a single genogroup (pulsotype A), and GREfs were clustered in six genogroups (pulsotypes B-G). Among the isolates investigated by MLST, only 18 PCR products were sequenced (12 E. faecium and 6 E. faecalis), and 9 sequence types (STs) were identified.http://dx.doi.org/10.1155/2010/150464 |
| spellingShingle | M. Biendo C. Adjidé S. Castelain M. Belmekki F. Rousseau M. Slama O. Ganry J. L. Schmit F. Eb Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) International Journal of Microbiology |
| title | Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) |
| title_full | Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) |
| title_fullStr | Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) |
| title_full_unstemmed | Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) |
| title_short | Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France) |
| title_sort | molecular characterization of glycopeptide resistant enterococci from hospitals of the picardy region france |
| url | http://dx.doi.org/10.1155/2010/150464 |
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