Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice
Our earlier work showed that hydromethanol extract (HME) of Pleurotus florida had antioxidant and anti-cholinesterase potential. This study aimed at isolating the constituent responsible for the activities. HME was subjected to bioactivity guided fractionation using in vitro anti-acetylcholinesteras...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2021-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000586 |
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| author | Kudrat Randhawa Varinder Singh Sanimardeep Kaur Ravinder Kaur Suresh Kumar Richa Shri |
| author_facet | Kudrat Randhawa Varinder Singh Sanimardeep Kaur Ravinder Kaur Suresh Kumar Richa Shri |
| author_sort | Kudrat Randhawa |
| collection | DOAJ |
| description | Our earlier work showed that hydromethanol extract (HME) of Pleurotus florida had antioxidant and anti-cholinesterase potential. This study aimed at isolating the constituent responsible for the activities. HME was subjected to bioactivity guided fractionation using in vitro anti-acetylcholinesterase (Ellman method) and antioxidant (DPPH scavenging assay). The most active constituent was evaluated in vivo employing i.c.v. streptozotocin (STZ) induced dementia in mice. Morris water maze test was used for evaluating memory, followed by biochemical estimations and histopathological studies. Bioactivity guided fractionation resulted in isolation of resveratrol (identified by IR, NMR and MS) and its content in P. florida fruiting bodies was 0.0098% (m/m, by a validated TLC densitometric method). It improved STZ induced dementia and neurodegeneration in mice by reducing brain acetylcholinesterase action and oxidative stress. The observed effects might be the presence of resveratrol. Our results further endorse that P. florida derived resveratrol can be a promising therapy for Alzheimer's disease. |
| format | Article |
| id | doaj-art-2eb344b7b82146dfab14c36df33c91d6 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2eb344b7b82146dfab14c36df33c91d62025-02-03T10:38:35ZengTsinghua University PressFood Science and Human Wellness2213-45302021-07-01104490496Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in miceKudrat Randhawa0Varinder Singh1Sanimardeep Kaur2Ravinder Kaur3Suresh Kumar4Richa Shri5Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, IndiaChitkara College of Pharmacy, Chitkara University, Punjab, India; Corresponding author.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, IndiaDepartment of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, IndiaDepartment of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, IndiaDepartment of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India; Corresponding author.Our earlier work showed that hydromethanol extract (HME) of Pleurotus florida had antioxidant and anti-cholinesterase potential. This study aimed at isolating the constituent responsible for the activities. HME was subjected to bioactivity guided fractionation using in vitro anti-acetylcholinesterase (Ellman method) and antioxidant (DPPH scavenging assay). The most active constituent was evaluated in vivo employing i.c.v. streptozotocin (STZ) induced dementia in mice. Morris water maze test was used for evaluating memory, followed by biochemical estimations and histopathological studies. Bioactivity guided fractionation resulted in isolation of resveratrol (identified by IR, NMR and MS) and its content in P. florida fruiting bodies was 0.0098% (m/m, by a validated TLC densitometric method). It improved STZ induced dementia and neurodegeneration in mice by reducing brain acetylcholinesterase action and oxidative stress. The observed effects might be the presence of resveratrol. Our results further endorse that P. florida derived resveratrol can be a promising therapy for Alzheimer's disease.http://www.sciencedirect.com/science/article/pii/S2213453021000586Acetylcholinesterase inhibitorAlzheimer's diseaseMushroomsPleurotus floridaResveratrol |
| spellingShingle | Kudrat Randhawa Varinder Singh Sanimardeep Kaur Ravinder Kaur Suresh Kumar Richa Shri Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice Food Science and Human Wellness Acetylcholinesterase inhibitor Alzheimer's disease Mushrooms Pleurotus florida Resveratrol |
| title | Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice |
| title_full | Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice |
| title_fullStr | Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice |
| title_full_unstemmed | Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice |
| title_short | Isolation of Pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice |
| title_sort | isolation of pleurotus florida derived acetylcholinesterase inhibitor for the treatment of cognitive dysfunction in mice |
| topic | Acetylcholinesterase inhibitor Alzheimer's disease Mushrooms Pleurotus florida Resveratrol |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000586 |
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