Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates
Background and objectives: Multi-drug resistant microorganisms have caused a remarkable increase in hospital-acquired infections (HAI) during the previous few decades. Moreover, microbiological contamination of antiseptics and disinfectants has been recorded in medical settings. Methods: A total of...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Erbil Polytechnic University
2023-09-01
|
| Series: | Polytechnic Journal |
| Subjects: | |
| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol12/iss2/9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background and objectives: Multi-drug resistant microorganisms have caused a remarkable
increase in hospital-acquired infections (HAI) during the previous few decades. Moreover,
microbiological contamination of antiseptics and disinfectants has been recorded in medical settings.
Methods: A total of 61 clinically relevant Acinetobacter baumannii isolates were collected from
various clinical specimens in hospitals in Erbil city/Iraq between September 2021 and March 2022.
The isolates were identified using VITEK-2 compact system. Antimicrobial susceptibility testing was
carried out for a total of 16 different therapeutically relevant antibiotics in accordance with the criteria
of the Clinical Laboratory Standards Institute (CLSI) using the above mentioned system. A
spectrophotometer was used to determine the optical density at 600 nm (OD600nm) for biofilm
evaluation. Wilcoxon signed ranks test used to determine the effect of sub-MIC of CHX on the CHXMIC and biofilm formation.
Results: the study showed that there is a significant difference in CHX-MIC and biofilm formation
before and after 7 days of incubation of Acinetobacter baumannii in sub-MIC of CHX (p<0.05).
Exposure of Acinetobacter baumannii to sub-lethal CHX concentrations develops inducible resistance
to lethal CHX doses. The biofilm formation ability of isolates also increased after using sub-lethal dose
of CHX. |
|---|---|
| ISSN: | 2707-7799 |