Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease
E-selectin (CD62E) is an endothelial specific glycoprotein belonging to the selectin family of adhesion molecules. Because a high expression of this molecule at intestinal mucosal surfaces in inflammatory bowel disease (IBD) has been described earlier, the aim was to assess serum levels of E-selecti...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S096293519400030X |
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| author | B. Vainerc O. H. Nielsen |
| author_facet | B. Vainerc O. H. Nielsen |
| author_sort | B. Vainerc |
| collection | DOAJ |
| description | E-selectin (CD62E) is an endothelial specific glycoprotein belonging
to the selectin family of adhesion molecules. Because a high
expression of this molecule at intestinal mucosal surfaces in
inflammatory bowel disease (IBD) has been described earlier, the aim
was to assess serum levels of E-selectin (sE-selectin) and to
correlate it to disease activity, and further to evaluate its
chemotactic properties at physiological concentrations. Levels of
sEselectin were measured by a sandwich ELISA technique in 31 IBD
patients together with 15 healthy volunteers. In ulcerative colitis
the median value was 0.46 nM (0.16–0.75), in Crohn’s disease
0.47 nM (0.22–1.24), and in healthy controls 0.34 nM
(0.22–0.83). No statistically significant differences in
sE-selectin were revealed between these groups (p>0.05). The in vitro chemotactic capabilities of E-selectin (in the
concentration range of 0.10–31.4 nM) were assessed using the
leading front technique. A significantly increased migratory
response was found at concentrations of 1.00 (p<0.05) and 3.14 nM (p<0.02). It is concluded that sE-selectin in
contrast to sICAM-1 does not act as a sensitive indicator of local
immune activation in IBD. However, E-selectin may be important for
recruitment and accumulation of neutrophilic granulocytes and other
phagocytes involved in the inflammatory process seen in IBD. Future
investigations are encouraged in order to reveal its in
vivo effects. |
| format | Article |
| id | doaj-art-2e393678b5c74687823e50585669e98b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2e393678b5c74687823e50585669e98b2025-02-03T05:44:23ZengWileyMediators of Inflammation0962-93511466-18611994-01-013321521810.1155/S096293519400030XSerum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel DiseaseB. Vainerc0O. H. Nielsen1Department of Medical Gastroenterology C, Herlev Hospital, University of Copenhagen, Herlev Ringvej, Herlev, Copenhagen DK-2730, DenmarkDepartment of Medical Gastroenterology C, Herlev Hospital, University of Copenhagen, Herlev Ringvej, Herlev, Copenhagen DK-2730, DenmarkE-selectin (CD62E) is an endothelial specific glycoprotein belonging to the selectin family of adhesion molecules. Because a high expression of this molecule at intestinal mucosal surfaces in inflammatory bowel disease (IBD) has been described earlier, the aim was to assess serum levels of E-selectin (sE-selectin) and to correlate it to disease activity, and further to evaluate its chemotactic properties at physiological concentrations. Levels of sEselectin were measured by a sandwich ELISA technique in 31 IBD patients together with 15 healthy volunteers. In ulcerative colitis the median value was 0.46 nM (0.16–0.75), in Crohn’s disease 0.47 nM (0.22–1.24), and in healthy controls 0.34 nM (0.22–0.83). No statistically significant differences in sE-selectin were revealed between these groups (p>0.05). The in vitro chemotactic capabilities of E-selectin (in the concentration range of 0.10–31.4 nM) were assessed using the leading front technique. A significantly increased migratory response was found at concentrations of 1.00 (p<0.05) and 3.14 nM (p<0.02). It is concluded that sE-selectin in contrast to sICAM-1 does not act as a sensitive indicator of local immune activation in IBD. However, E-selectin may be important for recruitment and accumulation of neutrophilic granulocytes and other phagocytes involved in the inflammatory process seen in IBD. Future investigations are encouraged in order to reveal its in vivo effects.http://dx.doi.org/10.1155/S096293519400030X |
| spellingShingle | B. Vainerc O. H. Nielsen Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease Mediators of Inflammation |
| title | Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease |
| title_full | Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease |
| title_fullStr | Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease |
| title_full_unstemmed | Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease |
| title_short | Serum Concentration and Chemotactic Activity of E-selectin (CD62E) in Inflammatory Bowel Disease |
| title_sort | serum concentration and chemotactic activity of e selectin cd62e in inflammatory bowel disease |
| url | http://dx.doi.org/10.1155/S096293519400030X |
| work_keys_str_mv | AT bvainerc serumconcentrationandchemotacticactivityofeselectincd62eininflammatoryboweldisease AT ohnielsen serumconcentrationandchemotacticactivityofeselectincd62eininflammatoryboweldisease |