Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears
Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Molecular Metabolism |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877824002151 |
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| author | Robbert J. Van der Pijl Weikang Ma Christopher T.A. Lewis Line Haar Amalie Buhl Gerrie P. Farman Marcus Rhodehamel Vivek P. Jani O Lynne Nelson Chengxin Zhang Henk Granzier Julien Ochala |
| author_facet | Robbert J. Van der Pijl Weikang Ma Christopher T.A. Lewis Line Haar Amalie Buhl Gerrie P. Farman Marcus Rhodehamel Vivek P. Jani O Lynne Nelson Chengxin Zhang Henk Granzier Julien Ochala |
| author_sort | Robbert J. Van der Pijl |
| collection | DOAJ |
| description | Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function. |
| format | Article |
| id | doaj-art-2e3600a033324036b1df8a4522aeb3f4 |
| institution | Kabale University |
| issn | 2212-8778 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj-art-2e3600a033324036b1df8a4522aeb3f42025-02-01T04:11:57ZengElsevierMolecular Metabolism2212-87782025-02-0192102084Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bearsRobbert J. Van der Pijl0Weikang Ma1Christopher T.A. Lewis2Line Haar3Amalie Buhl4Gerrie P. Farman5Marcus Rhodehamel6Vivek P. Jani7O Lynne Nelson8Chengxin Zhang9Henk Granzier10Julien Ochala11Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USABioCAT, Department of Biology, Illinois Institute of Technology, Chicago, IL, USADepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USADivision of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USADivision of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USACollege of Veterinary Medicine, Washington State University, Pullman, WA, USADepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USADepartment of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USADepartment of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark; Myocardial Homeostasis and Cardiac Injury Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Corresponding author. Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.http://www.sciencedirect.com/science/article/pii/S2212877824002151HibernationHeartMyosinMetabolism |
| spellingShingle | Robbert J. Van der Pijl Weikang Ma Christopher T.A. Lewis Line Haar Amalie Buhl Gerrie P. Farman Marcus Rhodehamel Vivek P. Jani O Lynne Nelson Chengxin Zhang Henk Granzier Julien Ochala Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears Molecular Metabolism Hibernation Heart Myosin Metabolism |
| title | Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears |
| title_full | Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears |
| title_fullStr | Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears |
| title_full_unstemmed | Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears |
| title_short | Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears |
| title_sort | increased cardiac myosin super relaxation as an energy saving mechanism in hibernating grizzly bears |
| topic | Hibernation Heart Myosin Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2212877824002151 |
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