New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing c...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/9465028 |
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| _version_ | 1832551868720480256 |
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| author | Xiangjun He Yin-Xiong Li Bo Feng |
| author_facet | Xiangjun He Yin-Xiong Li Bo Feng |
| author_sort | Xiangjun He |
| collection | DOAJ |
| description | The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC. |
| format | Article |
| id | doaj-art-2e27d4096fa14cea954b258f979373e5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-2e27d4096fa14cea954b258f979373e52025-02-03T06:00:19ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/94650289465028New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem CellsXiangjun He0Yin-Xiong Li1Bo Feng2Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong KongInstitute of Public Health, Guangdong Provincial Key Laboratory of Biocomputing, Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong KongThe groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC.http://dx.doi.org/10.1155/2018/9465028 |
| spellingShingle | Xiangjun He Yin-Xiong Li Bo Feng New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells Stem Cells International |
| title | New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells |
| title_full | New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells |
| title_fullStr | New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells |
| title_full_unstemmed | New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells |
| title_short | New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells |
| title_sort | new turns for high efficiency knock in of large dna in human pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2018/9465028 |
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