Effects of Biostabilization on Engineering Properties of Geomaterials

Biostabilization is a newly proposed method to improve the strength and durability of geomaterials, and it can serve as an alternative to chemical and mechanical stabilization. The objectives of this study are to perform biostabilization treatments for selected roadway construction geomaterials and...

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Main Authors: Shengting Li, Chenyi Luo, Yi Yang, Lvzhen Yang, Lijian Wu, Tuo Huang, Zhuangji Wang
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Advances in Civil Engineering
Online Access:http://dx.doi.org/10.1155/2021/6654213
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author Shengting Li
Chenyi Luo
Yi Yang
Lvzhen Yang
Lijian Wu
Tuo Huang
Zhuangji Wang
author_facet Shengting Li
Chenyi Luo
Yi Yang
Lvzhen Yang
Lijian Wu
Tuo Huang
Zhuangji Wang
author_sort Shengting Li
collection DOAJ
description Biostabilization is a newly proposed method to improve the strength and durability of geomaterials, and it can serve as an alternative to chemical and mechanical stabilization. The objectives of this study are to perform biostabilization treatments for selected roadway construction geomaterials and to evaluate the biostabilization effects on engineering properties of the geomaterials. Three types of geomaterials were selected, and two of them were compacted soil from unpaved road surface. Bacillus pasteurii, the biostabilization bacterium, was used to induce mineral precipitates within the geomaterial pore spaces, where the biostabilization effects were performed. Two types of liquid incubation media, containing NH4Cl or (NH4)2 SO4, were applied for bacteria culturing. Unconfined compression, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) measurements were conducted to evaluate the biostabilization results. From unconfined compression, sample strength performance was improved by the biostabilization treatments; the benefits of biostabilization were pronounced by a relatively long culturing time and an oven-dry procedure; the liquid culturing medium containing NH4Cl performed better than the medium containing (NH4)2 SO4. After biostabilization, SEM photographs provided direct evidence for the precipitates induced by bacteria within the geomaterial pore space. The precipitates either connected the adjoined particles or partially covered the particle surface, which increased the surface roughness. EDS and XRD results indicated that calcite, dolomite, and albite were the major precipitates produced during biostabilization treatments. In conclusion, biostabilization ameliorated the microstructures of the geomaterials and improved their strength. Future research topics should include the applications of biostabilization for in situ road construction.
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spelling doaj-art-2e1d68951d424314aa2eefecab690ed52025-02-03T01:05:30ZengWileyAdvances in Civil Engineering1687-80861687-80942021-01-01202110.1155/2021/66542136654213Effects of Biostabilization on Engineering Properties of GeomaterialsShengting Li0Chenyi Luo1Yi Yang2Lvzhen Yang3Lijian Wu4Tuo Huang5Zhuangji Wang6Key Laboratory of Transport Industry of Road Structure and Material, Ministry of Transport, Research Institute of Highway, Beijing 100088, ChinaSchool of Life Science, Beijing University of Chinese Medicine, Beijing 102488, ChinaXiandai Investment Co., Ltd., School of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha, Hunan 410076, ChinaSchool of Road and Bridge Engineering, Hunan Communication Polytechnic, Huangxing Zhen, Changsha, Hunan 410132, ChinaKey Laboratory of Transport Industry of Road Structure and Material, Ministry of Transport, Research Institute of Highway, Beijing 100088, ChinaSchool of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha, Hunan 410076, ChinaDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USABiostabilization is a newly proposed method to improve the strength and durability of geomaterials, and it can serve as an alternative to chemical and mechanical stabilization. The objectives of this study are to perform biostabilization treatments for selected roadway construction geomaterials and to evaluate the biostabilization effects on engineering properties of the geomaterials. Three types of geomaterials were selected, and two of them were compacted soil from unpaved road surface. Bacillus pasteurii, the biostabilization bacterium, was used to induce mineral precipitates within the geomaterial pore spaces, where the biostabilization effects were performed. Two types of liquid incubation media, containing NH4Cl or (NH4)2 SO4, were applied for bacteria culturing. Unconfined compression, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) measurements were conducted to evaluate the biostabilization results. From unconfined compression, sample strength performance was improved by the biostabilization treatments; the benefits of biostabilization were pronounced by a relatively long culturing time and an oven-dry procedure; the liquid culturing medium containing NH4Cl performed better than the medium containing (NH4)2 SO4. After biostabilization, SEM photographs provided direct evidence for the precipitates induced by bacteria within the geomaterial pore space. The precipitates either connected the adjoined particles or partially covered the particle surface, which increased the surface roughness. EDS and XRD results indicated that calcite, dolomite, and albite were the major precipitates produced during biostabilization treatments. In conclusion, biostabilization ameliorated the microstructures of the geomaterials and improved their strength. Future research topics should include the applications of biostabilization for in situ road construction.http://dx.doi.org/10.1155/2021/6654213
spellingShingle Shengting Li
Chenyi Luo
Yi Yang
Lvzhen Yang
Lijian Wu
Tuo Huang
Zhuangji Wang
Effects of Biostabilization on Engineering Properties of Geomaterials
Advances in Civil Engineering
title Effects of Biostabilization on Engineering Properties of Geomaterials
title_full Effects of Biostabilization on Engineering Properties of Geomaterials
title_fullStr Effects of Biostabilization on Engineering Properties of Geomaterials
title_full_unstemmed Effects of Biostabilization on Engineering Properties of Geomaterials
title_short Effects of Biostabilization on Engineering Properties of Geomaterials
title_sort effects of biostabilization on engineering properties of geomaterials
url http://dx.doi.org/10.1155/2021/6654213
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