Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8
Objective: To investigate the value of 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid ([ 18 F]ML-8) positron emission tomography (PET) imaging of rat pulmonary fibrosis. Methods: Male Sprague-Dawley rats were divided into 2 groups, including pulmonary fibrosis model group and control group. The rat...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012118795728 |
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| author | Ying Xiong MM Dahong Nie BM Shaoyu Liu PhD Hui Ma BM Shu Su BM Aixia Sun PhD Jing Zhao MD, PhD Zhanwen Zhang PhD Xianhong Xiang MD, PhD Ganghua Tang MD, PhD |
| author_facet | Ying Xiong MM Dahong Nie BM Shaoyu Liu PhD Hui Ma BM Shu Su BM Aixia Sun PhD Jing Zhao MD, PhD Zhanwen Zhang PhD Xianhong Xiang MD, PhD Ganghua Tang MD, PhD |
| author_sort | Ying Xiong MM |
| collection | DOAJ |
| description | Objective: To investigate the value of 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid ([ 18 F]ML-8) positron emission tomography (PET) imaging of rat pulmonary fibrosis. Methods: Male Sprague-Dawley rats were divided into 2 groups, including pulmonary fibrosis model group and control group. The rat model was established by an intratracheal instillation of bleomycin (BLM). Control rats were treated with saline. Positron emission tomography/computed tomography (CT) with [ 18 F]ML-8 or 18 F-fluorodeoxyglucose ([ 18 F]FDG) was performed on 2 groups. After PET/CT imaging, lung tissues were collected for histologic examination. Data were analyzed and comparisons between 2 groups were performed using Student t test. Results: Bleomycin-treated rats showed a higher lung uptake of [ 18 F]ML-8 than control rats ( P < .05). In BLM-treated rats, the lung to muscle relative uptake ratio of [ 18 F]ML-8 was also higher than that of [ 18 F]FDG ( P < .05). Pathological examination showed overproliferation of fibroblasts and deposition of collagen in lungs from BLM-treated rats. Compared to control rats, BLM-treated rats had higher lung hydroxyproline content ( P < .05). Immunofluorescence staining indicated more apoptotic cells in BLM-treated rats than those in control rats. Moreover, the apoptosis rate of lung tissues obtained from BLM-treated rats was higher than that from control rats ( P < .05). Conclusions: 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid PET/CT could be used for noninvasive diagnosis of pulmonary fibrosis in a rat model. |
| format | Article |
| id | doaj-art-2e197880e21045008c364af361204a3d |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2018-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-2e197880e21045008c364af361204a3d2025-02-03T10:07:59ZengSAGE PublishingMolecular Imaging1536-01212018-10-011710.1177/1536012118795728Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8Ying Xiong MM0Dahong Nie BM1Shaoyu Liu PhD2Hui Ma BM3Shu Su BM4Aixia Sun PhD5Jing Zhao MD, PhD6Zhanwen Zhang PhD7Xianhong Xiang MD, PhD8Ganghua Tang MD, PhD9 Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals and Department of Medical Imaging, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaObjective: To investigate the value of 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid ([ 18 F]ML-8) positron emission tomography (PET) imaging of rat pulmonary fibrosis. Methods: Male Sprague-Dawley rats were divided into 2 groups, including pulmonary fibrosis model group and control group. The rat model was established by an intratracheal instillation of bleomycin (BLM). Control rats were treated with saline. Positron emission tomography/computed tomography (CT) with [ 18 F]ML-8 or 18 F-fluorodeoxyglucose ([ 18 F]FDG) was performed on 2 groups. After PET/CT imaging, lung tissues were collected for histologic examination. Data were analyzed and comparisons between 2 groups were performed using Student t test. Results: Bleomycin-treated rats showed a higher lung uptake of [ 18 F]ML-8 than control rats ( P < .05). In BLM-treated rats, the lung to muscle relative uptake ratio of [ 18 F]ML-8 was also higher than that of [ 18 F]FDG ( P < .05). Pathological examination showed overproliferation of fibroblasts and deposition of collagen in lungs from BLM-treated rats. Compared to control rats, BLM-treated rats had higher lung hydroxyproline content ( P < .05). Immunofluorescence staining indicated more apoptotic cells in BLM-treated rats than those in control rats. Moreover, the apoptosis rate of lung tissues obtained from BLM-treated rats was higher than that from control rats ( P < .05). Conclusions: 2-(3-[ 18 F]fluoropropyl)-2-methyl-malonic acid PET/CT could be used for noninvasive diagnosis of pulmonary fibrosis in a rat model.https://doi.org/10.1177/1536012118795728 |
| spellingShingle | Ying Xiong MM Dahong Nie BM Shaoyu Liu PhD Hui Ma BM Shu Su BM Aixia Sun PhD Jing Zhao MD, PhD Zhanwen Zhang PhD Xianhong Xiang MD, PhD Ganghua Tang MD, PhD Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 Molecular Imaging |
| title | Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 |
| title_full | Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 |
| title_fullStr | Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 |
| title_full_unstemmed | Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 |
| title_short | Apoptotic PET Imaging of Rat Pulmonary Fibrosis With [F]ML-8 |
| title_sort | apoptotic pet imaging of rat pulmonary fibrosis with f ml 8 |
| url | https://doi.org/10.1177/1536012118795728 |
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