Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum
Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Co10) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresp...
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Zhejiang University Press
2004-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.04.0426 |
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| author | DAI Fu-ming XU Tong |
| author_facet | DAI Fu-ming XU Tong |
| author_sort | DAI Fu-ming |
| collection | DOAJ |
| description | Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Co10) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase (ThEn-42) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86.7%) 10 days after inoculation with the pathogen. |
| format | Article |
| id | doaj-art-2e041aa4c69b42e99062ac64ab2fc4bb |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-2e041aa4c69b42e99062ac64ab2fc4bb2025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-07-013042642610.3785/1008-9209.2004.04.042610089209Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorumDAI Fu-mingXU TongSclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Co10) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase (ThEn-42) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86.7%) 10 days after inoculation with the pathogen.https://www.academax.com/doi/10.3785/1008-9209.2004.04.0426<italic>Arabidopsis thaliana</italic><italic>Trichoderma</italic> endochitinase generesistance<italic>Sclerotinia sclerotiorum</italic> |
| spellingShingle | DAI Fu-ming XU Tong Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum 浙江大学学报. 农业与生命科学版 <italic>Arabidopsis thaliana</italic> <italic>Trichoderma</italic> endochitinase gene resistance <italic>Sclerotinia sclerotiorum</italic> |
| title | Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
| title_full | Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
| title_fullStr | Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
| title_full_unstemmed | Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
| title_short | Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
| title_sort | transformation of arabidopsis thaliana via agrobacterium tumefacience with an endochitinase gene from trichoderma and enhanced resistance to sclerotinia sclerotiorum |
| topic | <italic>Arabidopsis thaliana</italic> <italic>Trichoderma</italic> endochitinase gene resistance <italic>Sclerotinia sclerotiorum</italic> |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.04.0426 |
| work_keys_str_mv | AT daifuming transformationofarabidopsisthalianaviaagrobacteriumtumefaciencewithanendochitinasegenefromtrichodermaandenhancedresistancetosclerotiniasclerotiorum AT xutong transformationofarabidopsisthalianaviaagrobacteriumtumefaciencewithanendochitinasegenefromtrichodermaandenhancedresistancetosclerotiniasclerotiorum |