<i>Candida auris</i> Antifungal Resistance, Virulence and Susceptibility to a Novel Nitric Oxide-Releasing Microparticle and Its Correlations to Clade Identification

<i>Candida auris</i> Antifungal Resistance, Virulence and Susceptibility to a Novel Nitric Oxide-Releasing Microparticle and Its Correlations to Clade Identification

<i>Candida auris</i> is a globally emerging pathogenic fungus described in Japan in 2009. This fungus has been identified mainly in nosocomial environments, associated with a high frequency of multidrug-resistant strains, and mortality rates reach 60%. <i>C. auris</i> is divi...

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Main Authors: Alessandro F. Valdez, Flora Bohner, Joshua P. Goldman, Ali B. Jaquiery, Eduardo V. C. do Amaral, Dario Correa-Junior, Andrew Draganski, Leonardo Nimrichter, Joshua D. Nosanchuk, Daniel Zamith-Miranda
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Microbiology Research
Subjects:
<i>Candida auris</i>
resistance
antifungals
clades
microparticles
Online Access:https://www.mdpi.com/2036-7481/16/1/15
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Summary:<i>Candida auris</i> is a globally emerging pathogenic fungus described in Japan in 2009. This fungus has been identified mainly in nosocomial environments, associated with a high frequency of multidrug-resistant strains, and mortality rates reach 60%. <i>C. auris</i> is divided into 6 biogeographic clades, and there is a correlation between the clades and resistance against antifungals. In the current report, 8 strains of <i>C. auris</i> isolated in the Montefiore Medical Center, Bronx were analyzed to assess their clade (via ClaID) and common molecular determinants of antifungal resistance. We assessed antifungal resistance as well as the efficacy of a novel nitric oxide-donating microparticle as an alternative approach against <i>C. auris</i> in vitro through microplate susceptibility tests. Virulence was also determined in a <i>Galleria mellonella</i> model. Our results indicate that 7 out of 8 strains, belonging to clade 1, were resistant to fluconazole, while clade 2 was susceptible. Additionally, the clade 2 strain was more susceptible to treatment with the microparticle, while also being more virulent in an invertebrate model of infection. Our findings were then correlated to visualize parallels between clade identification and resistance/virulence patterns.
ISSN:2036-7481

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