Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells
The α1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y. In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gen...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/105464 |
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| author | Fumiko Taniuchi Koji Higai Tomomi Tanaka Yutaro Azuma Kojiro Matsumoto |
| author_facet | Fumiko Taniuchi Koji Higai Tomomi Tanaka Yutaro Azuma Kojiro Matsumoto |
| author_sort | Fumiko Taniuchi |
| collection | DOAJ |
| description | The α1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y. In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gene, and shows α1,2-fucosyltransferase (FUT) activity. 5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS). Using the dual luciferase assay, FUT1 gene expression was shown to be regulated at the region −91 to −81 nt to the transcriptional start site, which contains the Elk-1 binding site. Site-directed mutagenesis of this region revealed the Elk-1 binding site to be essential for FUT1 transcription. Furthermore, transfection of the dominant negative Elk-1 gene, and the chromatin immunoprecipitation (CHIp) assay, supported Elk-1-dependent transcriptional regulation of FUT1 gene expression in DLD-1 cells. These results suggest that a defined region in the 5′-flanking region of FUT1 is critical for FUT1 transcription and that constitutive gene expression of FUT1 is regulated by Elk-1 in DLD-1 cells. |
| format | Article |
| id | doaj-art-2de893a4b4104874a47fe93a5b20174b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2de893a4b4104874a47fe93a5b20174b2025-02-03T01:09:58ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/105464105464Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer CellsFumiko Taniuchi0Koji Higai1Tomomi Tanaka2Yutaro Azuma3Kojiro Matsumoto4Department of Medical Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, JapanDepartment of Medical Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, JapanDepartment of Medical Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, JapanDepartment of Medical Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, JapanDepartment of Medical Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, JapanThe α1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y. In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gene, and shows α1,2-fucosyltransferase (FUT) activity. 5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS). Using the dual luciferase assay, FUT1 gene expression was shown to be regulated at the region −91 to −81 nt to the transcriptional start site, which contains the Elk-1 binding site. Site-directed mutagenesis of this region revealed the Elk-1 binding site to be essential for FUT1 transcription. Furthermore, transfection of the dominant negative Elk-1 gene, and the chromatin immunoprecipitation (CHIp) assay, supported Elk-1-dependent transcriptional regulation of FUT1 gene expression in DLD-1 cells. These results suggest that a defined region in the 5′-flanking region of FUT1 is critical for FUT1 transcription and that constitutive gene expression of FUT1 is regulated by Elk-1 in DLD-1 cells.http://dx.doi.org/10.1155/2013/105464 |
| spellingShingle | Fumiko Taniuchi Koji Higai Tomomi Tanaka Yutaro Azuma Kojiro Matsumoto Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells The Scientific World Journal |
| title | Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells |
| title_full | Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells |
| title_fullStr | Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells |
| title_full_unstemmed | Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells |
| title_short | Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells |
| title_sort | transcriptional regulation of fucosyltransferase 1 gene expression in colon cancer cells |
| url | http://dx.doi.org/10.1155/2013/105464 |
| work_keys_str_mv | AT fumikotaniuchi transcriptionalregulationoffucosyltransferase1geneexpressionincoloncancercells AT kojihigai transcriptionalregulationoffucosyltransferase1geneexpressionincoloncancercells AT tomomitanaka transcriptionalregulationoffucosyltransferase1geneexpressionincoloncancercells AT yutaroazuma transcriptionalregulationoffucosyltransferase1geneexpressionincoloncancercells AT kojiromatsumoto transcriptionalregulationoffucosyltransferase1geneexpressionincoloncancercells |