Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels

Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels

Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to <i>Vibrio</i> contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of <i>Vibrio</i> spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-e...

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Bibliographic Details
Main Authors: Artun Yibar, Muhammed Duman, Hilal Ay, Nihed Ajmi, Gorkem Tasci, Fatma Gurler, Sabire Guler, Danny Morick, Izzet Burcin Saticioglu
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Pathogens
Subjects:
whole-genome sequencing
foodborne pathogen
mussel
public health
<i>Vibrio</i> spp.
Online Access:https://www.mdpi.com/2076-0817/14/1/52
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Summary:Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to <i>Vibrio</i> contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of <i>Vibrio</i> spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks. The results showed <i>Vibrio</i> prevalence rates of 12.5% in FRMs and 4.2% in RTE-SMs, with <i>V. alginolyticus</i> as the most common species (46.7%). It was determined that the seasonal distribution of <i>Vibrio</i> spp. prevalence in the samples was higher in the summer months. The genome sizes of the <i>Vibrio</i> spp. ranged from approximately 3.9 to 6.1 Mb, with the GC contents varying between 41.9% and 50.4%. A total of 22 virulence factor (VF) classes and up to six antimicrobial resistance (AMR) genes were detected in different <i>Vibrio</i> species. The presence of nine different biosynthetic gene clusters (BGCs), 27 prophage regions, and eight CRISPR/Cas systems in 15 <i>Vibrio</i> strains provides information about their potential pathogenicity, survival strategies, and adaptation to different habitats. Overall, this study provides a comprehensive understanding of the genomic diversity of <i>Vibrio</i> spp. isolated from FRM and RTE-SM samples, shedding light on the prevalence, pathogenicity, and toxicity mechanisms of <i>Vibrio</i>-induced gastroenteritis.
ISSN:2076-0817

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