Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography
The retention mechanism of four major carotenoids, two xanthophylls (i.e., lutein and zeaxanthin) and two carotenes (i.e., lycopene and β-carotene), was investigated in reversed-phase liquid chromatography with the aim of thermodynamic analysis. The experimental variables considered in this study we...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/7535813 |
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| author | Nicola Marchetti Pier Paolo Giovannini Martina Catani Luisa Pasti Alberto Cavazzini |
| author_facet | Nicola Marchetti Pier Paolo Giovannini Martina Catani Luisa Pasti Alberto Cavazzini |
| author_sort | Nicola Marchetti |
| collection | DOAJ |
| description | The retention mechanism of four major carotenoids, two xanthophylls (i.e., lutein and zeaxanthin) and two carotenes (i.e., lycopene and β-carotene), was investigated in reversed-phase liquid chromatography with the aim of thermodynamic analysis. The experimental variables considered in this study were the composition of mobile phase (MP) and the temperature. Chromatographic elutions were undertaken under linear, isocratic conditions by using a C18 stationary phase, four different MP compositions (by varying the ratio methanol/acetonitrile from 66.5/28.5 to 47.5/47.5 v/v), and column temperatures in the range 283–313 K. Traditional Van’t Hoff analysis has been used to estimate changes of standard enthalpy (ΔH∘) and Gibbs free energy (ΔG∘) associated with the solute transfer from the mobile to the stationary phase at each mobile phase composition. The thermodynamic quantities have been correlated to the structure of investigated carotenoids and their interaction with the octadecyl silica stationary phase. |
| format | Article |
| id | doaj-art-2dcda46c98574614b16d72a2a5f29e8f |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-2dcda46c98574614b16d72a2a5f29e8f2025-02-03T06:00:22ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/75358137535813Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid ChromatographyNicola Marchetti0Pier Paolo Giovannini1Martina Catani2Luisa Pasti3Alberto Cavazzini4Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, ItalyDepartment of Chemistry and Pharmaceutical Sciences, University of Ferrara, ItalyDepartment of Chemistry and Pharmaceutical Sciences, University of Ferrara, ItalyDepartment of Chemistry and Pharmaceutical Sciences, University of Ferrara, ItalyDepartment of Chemistry and Pharmaceutical Sciences, University of Ferrara, ItalyThe retention mechanism of four major carotenoids, two xanthophylls (i.e., lutein and zeaxanthin) and two carotenes (i.e., lycopene and β-carotene), was investigated in reversed-phase liquid chromatography with the aim of thermodynamic analysis. The experimental variables considered in this study were the composition of mobile phase (MP) and the temperature. Chromatographic elutions were undertaken under linear, isocratic conditions by using a C18 stationary phase, four different MP compositions (by varying the ratio methanol/acetonitrile from 66.5/28.5 to 47.5/47.5 v/v), and column temperatures in the range 283–313 K. Traditional Van’t Hoff analysis has been used to estimate changes of standard enthalpy (ΔH∘) and Gibbs free energy (ΔG∘) associated with the solute transfer from the mobile to the stationary phase at each mobile phase composition. The thermodynamic quantities have been correlated to the structure of investigated carotenoids and their interaction with the octadecyl silica stationary phase.http://dx.doi.org/10.1155/2019/7535813 |
| spellingShingle | Nicola Marchetti Pier Paolo Giovannini Martina Catani Luisa Pasti Alberto Cavazzini Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography International Journal of Analytical Chemistry |
| title | Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography |
| title_full | Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography |
| title_fullStr | Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography |
| title_full_unstemmed | Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography |
| title_short | Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography |
| title_sort | thermodynamic insights into the separation of carotenoids in reversed phase liquid chromatography |
| url | http://dx.doi.org/10.1155/2019/7535813 |
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