IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits
Genetic variations contribute to the susceptibility in the development of periodontitis. The aim of this study was to investigate the influence of IL18, IL12, and MMP9 polymorphisms in the chronic periodontitis. This case-control study involved 381 individuals matched by gender and age. Genotyping o...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/9585964 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564673250066432 |
|---|---|
| author | Patrícia Yumeko Tsuneto Victor Hugo de Souza Josiane Bazzo de Alencar Joana Maira Valentini Zacarias Cléverson O. Silva Jeane Eliete Laguila Visentainer Ana Maria Sell |
| author_facet | Patrícia Yumeko Tsuneto Victor Hugo de Souza Josiane Bazzo de Alencar Joana Maira Valentini Zacarias Cléverson O. Silva Jeane Eliete Laguila Visentainer Ana Maria Sell |
| author_sort | Patrícia Yumeko Tsuneto |
| collection | DOAJ |
| description | Genetic variations contribute to the susceptibility in the development of periodontitis. The aim of this study was to investigate the influence of IL18, IL12, and MMP9 polymorphisms in the chronic periodontitis. This case-control study involved 381 individuals matched by gender and age. Genotyping of IL18 (rs187238 and rs1946518) and IL12B (rs3212227) was performed by PCR-SSP and PCR-RFLP was used for MMP9 (rs3918242). IL-18 and MMP-9 were quantified in the serum by ELISA. SNPStats and OpenEpi software were used for statistical analysis and, in order to eliminate smoking as a confounding factor, the analyses were also performed in nonsmoking subjects. The IL18-137G/C genotype was associated with the risk of chronic periodontitis in nonsmokers (Pc=0.03; OR=1.99; overdominant inherence model). In the multivariate analyses, homozygous IL18-137G/G and IL18-607C/C were more frequent in males compared to women with these same genotypes (OR=2.51 and OR=3.30, respectively). The serum levels of the IL-18 in patients were higher than those in healthy controls (P=0.005). IL12B and MMP9 polymorphisms and MMP-9 serum concentration were similar in patients and controls. In this study, IL18 was associated with chronic periodontitis susceptibility. Men had greater risk than women for developing the disease when IL18 polymorphism was considered and the susceptibility was independent of the smoking status. |
| format | Article |
| id | doaj-art-2dbcd51252a749e597cc4c720865972e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2dbcd51252a749e597cc4c720865972e2025-02-03T01:10:26ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/95859649585964IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking HabitsPatrícia Yumeko Tsuneto0Victor Hugo de Souza1Josiane Bazzo de Alencar2Joana Maira Valentini Zacarias3Cléverson O. Silva4Jeane Eliete Laguila Visentainer5Ana Maria Sell6Post Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, BrazilPost Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, BrazilPost Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, BrazilPost Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, BrazilDepartment of Dentistry, Maringá State University, Paraná, BrazilPost Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, BrazilPost Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, BrazilGenetic variations contribute to the susceptibility in the development of periodontitis. The aim of this study was to investigate the influence of IL18, IL12, and MMP9 polymorphisms in the chronic periodontitis. This case-control study involved 381 individuals matched by gender and age. Genotyping of IL18 (rs187238 and rs1946518) and IL12B (rs3212227) was performed by PCR-SSP and PCR-RFLP was used for MMP9 (rs3918242). IL-18 and MMP-9 were quantified in the serum by ELISA. SNPStats and OpenEpi software were used for statistical analysis and, in order to eliminate smoking as a confounding factor, the analyses were also performed in nonsmoking subjects. The IL18-137G/C genotype was associated with the risk of chronic periodontitis in nonsmokers (Pc=0.03; OR=1.99; overdominant inherence model). In the multivariate analyses, homozygous IL18-137G/G and IL18-607C/C were more frequent in males compared to women with these same genotypes (OR=2.51 and OR=3.30, respectively). The serum levels of the IL-18 in patients were higher than those in healthy controls (P=0.005). IL12B and MMP9 polymorphisms and MMP-9 serum concentration were similar in patients and controls. In this study, IL18 was associated with chronic periodontitis susceptibility. Men had greater risk than women for developing the disease when IL18 polymorphism was considered and the susceptibility was independent of the smoking status.http://dx.doi.org/10.1155/2019/9585964 |
| spellingShingle | Patrícia Yumeko Tsuneto Victor Hugo de Souza Josiane Bazzo de Alencar Joana Maira Valentini Zacarias Cléverson O. Silva Jeane Eliete Laguila Visentainer Ana Maria Sell IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits Mediators of Inflammation |
| title | IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits |
| title_full | IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits |
| title_fullStr | IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits |
| title_full_unstemmed | IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits |
| title_short | IL18 Polymorphism and Periodontitis Susceptibility, Regardless of IL12B, MMP9, and Smoking Habits |
| title_sort | il18 polymorphism and periodontitis susceptibility regardless of il12b mmp9 and smoking habits |
| url | http://dx.doi.org/10.1155/2019/9585964 |
| work_keys_str_mv | AT patriciayumekotsuneto il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits AT victorhugodesouza il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits AT josianebazzodealencar il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits AT joanamairavalentinizacarias il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits AT cleversonosilva il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits AT jeaneelietelaguilavisentainer il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits AT anamariasell il18polymorphismandperiodontitissusceptibilityregardlessofil12bmmp9andsmokinghabits |