Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea
To investigate the effects of loss of transient receptor potential vanilloid receptor 1 (TRPV1) on the development of neovascularization in corneal stroma in mice. Blocking TRPV1 receptor did not affect VEGF-dependent neovascularization in cell culture. Lacking TRPV1 inhibited neovascularization in...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/706404 |
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| _version_ | 1832547492378443776 |
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| author | Katsuo Tomoyose Yuka Okada Takayoshi Sumioka Masayasu Miyajima Kathleen C. Flanders Kumi Shirai Tomoya Morii Peter S. Reinach Osamu Yamanaka Shizuya Saika |
| author_facet | Katsuo Tomoyose Yuka Okada Takayoshi Sumioka Masayasu Miyajima Kathleen C. Flanders Kumi Shirai Tomoya Morii Peter S. Reinach Osamu Yamanaka Shizuya Saika |
| author_sort | Katsuo Tomoyose |
| collection | DOAJ |
| description | To investigate the effects of loss of transient receptor potential vanilloid receptor 1 (TRPV1) on the development of neovascularization in corneal stroma in mice. Blocking TRPV1 receptor did not affect VEGF-dependent neovascularization in cell culture. Lacking TRPV1 inhibited neovascularization in corneal stroma following cauterization. Immunohistochemistry showed that immunoreactivity for active form of TGFβ1 and VEGF was detected in subepithelial stroma at the site of cauterization in both genotypes of mice, but the immunoreactivity seemed less marked in mice lacking TRPV1. mRNA expression of VEGF and TGFβ1 in a mouse cornea was suppressed by the loss of TRPV1. TRPV1 gene ablation did not affect invasion of neutrophils and macrophage in a cauterized mouse cornea. Blocking TRPV1 signal does not affect angiogenic effects by HUVECs in vitro. TRPV1 signal is, however, involved in expression of angiogenic growth factors in a cauterized mouse cornea and is required for neovascularization in the corneal stroma in vivo. |
| format | Article |
| id | doaj-art-2d8b0992c35743cf9e509aabb8f2ce1e |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-2d8b0992c35743cf9e509aabb8f2ce1e2025-02-03T06:44:32ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/706404706404Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse CorneaKatsuo Tomoyose0Yuka Okada1Takayoshi Sumioka2Masayasu Miyajima3Kathleen C. Flanders4Kumi Shirai5Tomoya Morii6Peter S. Reinach7Osamu Yamanaka8Shizuya Saika9Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanLaboratory Animal Center, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanLaboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USADepartment of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanWenzhou Medical University School of Ophthalmology and Optometry, Wenzhou, ChinaDepartment of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, JapanTo investigate the effects of loss of transient receptor potential vanilloid receptor 1 (TRPV1) on the development of neovascularization in corneal stroma in mice. Blocking TRPV1 receptor did not affect VEGF-dependent neovascularization in cell culture. Lacking TRPV1 inhibited neovascularization in corneal stroma following cauterization. Immunohistochemistry showed that immunoreactivity for active form of TGFβ1 and VEGF was detected in subepithelial stroma at the site of cauterization in both genotypes of mice, but the immunoreactivity seemed less marked in mice lacking TRPV1. mRNA expression of VEGF and TGFβ1 in a mouse cornea was suppressed by the loss of TRPV1. TRPV1 gene ablation did not affect invasion of neutrophils and macrophage in a cauterized mouse cornea. Blocking TRPV1 signal does not affect angiogenic effects by HUVECs in vitro. TRPV1 signal is, however, involved in expression of angiogenic growth factors in a cauterized mouse cornea and is required for neovascularization in the corneal stroma in vivo.http://dx.doi.org/10.1155/2015/706404 |
| spellingShingle | Katsuo Tomoyose Yuka Okada Takayoshi Sumioka Masayasu Miyajima Kathleen C. Flanders Kumi Shirai Tomoya Morii Peter S. Reinach Osamu Yamanaka Shizuya Saika Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea Journal of Ophthalmology |
| title | Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea |
| title_full | Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea |
| title_fullStr | Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea |
| title_full_unstemmed | Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea |
| title_short | Suppression of In Vivo Neovascularization by the Loss of TRPV1 in Mouse Cornea |
| title_sort | suppression of in vivo neovascularization by the loss of trpv1 in mouse cornea |
| url | http://dx.doi.org/10.1155/2015/706404 |
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