Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII
Newcastle disease viruses (NDVs) circulating among wild birds and poultry may differ in virulence. Some NDVs cause devastating outbreaks in chickens. The NDV/duck/Moscow/3639/2008 (d3639) strain was isolated from a wild duck. Its genome was sequenced (PP795281, GenBank) and the biological properties...
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MDPI AG
2025-04-01
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| author | Elizaveta Boravleva Anastasia Treshchalina Daria Gordeeva Alexandra Gambaryan Alla Belyakova Irina Gafarova Alexey Prilipov Galina Sadykova Simone Adams Tatiana Timofeeva Natalia Lomakina |
| author_facet | Elizaveta Boravleva Anastasia Treshchalina Daria Gordeeva Alexandra Gambaryan Alla Belyakova Irina Gafarova Alexey Prilipov Galina Sadykova Simone Adams Tatiana Timofeeva Natalia Lomakina |
| author_sort | Elizaveta Boravleva |
| collection | DOAJ |
| description | Newcastle disease viruses (NDVs) circulating among wild birds and poultry may differ in virulence. Some NDVs cause devastating outbreaks in chickens. The NDV/duck/Moscow/3639/2008 (d3639) strain was isolated from a wild duck. Its genome was sequenced (PP795281, GenBank) and the biological properties, specifically for infection in chicken and mice, were studied. Strain d3639 of genotype I.2 has an F protein cleavage site (112-GKQGRL-117) and a HN protein length (616 a.a.) of the lentogenic pathotype. It was tested, in comparison with the genotype II LaSota vaccine strain, for its immunogenicity and protective efficacy against a challenge with the velogenic NDV strain NDV/chicken/Moscow/6081/2022 (ch6081) of sub-genotype VII.1.1, the complete genome of which was also sequenced in this study (PP766718, GenBank). Both the d3639 and LaSota viruses did not induce clinical signs in chickens or mice. Single immunization was performed by inoculation through drinking water with the live virus. Inoculation protected the chickens during a subsequent challenge with velogenic ch6081 and significantly reduced shedding in feces. Double immunization was sufficient to achieve prolonged immunity and prevented the shedding of the velogenic virus after the challenge. Thus, this natural lentogenic d3639 virus possesses properties similar to the LaSota vaccine strain and can protect against sub-genotype VII.1.1 NDV. |
| format | Article |
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| issn | 2076-0817 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-2d83a388c33d475da865676a0b9c51a02025-08-20T02:18:15ZengMDPI AGPathogens2076-08172025-04-0114438010.3390/pathogens14040380Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VIIElizaveta Boravleva0Anastasia Treshchalina1Daria Gordeeva2Alexandra Gambaryan3Alla Belyakova4Irina Gafarova5Alexey Prilipov6Galina Sadykova7Simone Adams8Tatiana Timofeeva9Natalia Lomakina10Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaThe Scriabin and Kovalenko Federal Scientific Center for All-Russian Scientific Research Institute of Experimental Veterinary, The Russian Academy of Sciences, 109428 Moscow, RussiaThe Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, RussiaThe Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, RussiaInstitute of Microbiology, Lausanne University Hospital, 1011 Lausanne, SwitzerlandThe Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, RussiaThe Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, RussiaNewcastle disease viruses (NDVs) circulating among wild birds and poultry may differ in virulence. Some NDVs cause devastating outbreaks in chickens. The NDV/duck/Moscow/3639/2008 (d3639) strain was isolated from a wild duck. Its genome was sequenced (PP795281, GenBank) and the biological properties, specifically for infection in chicken and mice, were studied. Strain d3639 of genotype I.2 has an F protein cleavage site (112-GKQGRL-117) and a HN protein length (616 a.a.) of the lentogenic pathotype. It was tested, in comparison with the genotype II LaSota vaccine strain, for its immunogenicity and protective efficacy against a challenge with the velogenic NDV strain NDV/chicken/Moscow/6081/2022 (ch6081) of sub-genotype VII.1.1, the complete genome of which was also sequenced in this study (PP766718, GenBank). Both the d3639 and LaSota viruses did not induce clinical signs in chickens or mice. Single immunization was performed by inoculation through drinking water with the live virus. Inoculation protected the chickens during a subsequent challenge with velogenic ch6081 and significantly reduced shedding in feces. Double immunization was sufficient to achieve prolonged immunity and prevented the shedding of the velogenic virus after the challenge. Thus, this natural lentogenic d3639 virus possesses properties similar to the LaSota vaccine strain and can protect against sub-genotype VII.1.1 NDV.https://www.mdpi.com/2076-0817/14/4/380avian orthoavulavirus 1AOAV-1Newcastle disease virusNDVparamyxoviruspathogenesis |
| spellingShingle | Elizaveta Boravleva Anastasia Treshchalina Daria Gordeeva Alexandra Gambaryan Alla Belyakova Irina Gafarova Alexey Prilipov Galina Sadykova Simone Adams Tatiana Timofeeva Natalia Lomakina Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII Pathogens avian orthoavulavirus 1 AOAV-1 Newcastle disease virus NDV paramyxovirus pathogenesis |
| title | Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII |
| title_full | Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII |
| title_fullStr | Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII |
| title_full_unstemmed | Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII |
| title_short | Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII |
| title_sort | genotype i newcastle disease virus isolated from wild duck can protect chickens against newcastle disease caused by genotype vii |
| topic | avian orthoavulavirus 1 AOAV-1 Newcastle disease virus NDV paramyxovirus pathogenesis |
| url | https://www.mdpi.com/2076-0817/14/4/380 |
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