MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells
MicroRNA-140-5p (miR-140-5p) plays a pivotal role in human cancers. However, its role and molecular mechanisms in breast carcinoma are not fully explored. Using miR-140-5p transfected breast cancer cell line MDA-MB-231, several in vitro experiments were performed and described in this paper. They co...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-12-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0039 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548179462062080 |
|---|---|
| author | Hou Lingli Liu Qi Zhao Ying Yang Hongwei Meng Qingying Yu Fei |
| author_facet | Hou Lingli Liu Qi Zhao Ying Yang Hongwei Meng Qingying Yu Fei |
| author_sort | Hou Lingli |
| collection | DOAJ |
| description | MicroRNA-140-5p (miR-140-5p) plays a pivotal role in human cancers. However, its role and molecular mechanisms in breast carcinoma are not fully explored. Using miR-140-5p transfected breast cancer cell line MDA-MB-231, several in vitro experiments were performed and described in this paper. They consist of the cell proliferation assay, wound healing assay, transwell assay, colony formation assays and qRTPCR. Expression levels of target proteins were determined using Western blotting. In addition, experiments on animal models were performed to study the possible role of miR-140-5p in tumorigenesis of breast carcinoma cells. The induction of experimental breast tumor in mice model was achieved through the incorporation of MDA-MB-231 tumor cells subcutaneously into the middle left side of the mice. The results showed that miR-140-5p up-regulation significantly suppresses proliferation, cellular invasion and migration of breast carcinoma cells. Furthermore, miR-140-5p up-regulation stops breast cancer cells at G0/G1 phase. The results of the animal model indicated that up-regulation of miR-140-5p suppresses its tumorigenic ability. Moreover, we also found that miR-140-5p up-regulation reduces the phosphorylation level of STAT3, p65, and AKT. In addition, miR-140-5p overexpression significantly decreases CDK2 expression while increasing E-cadherin expression level. These data revealed that miR-140-5p suppressed tumor progression of breast carcinoma cells through inhibition of the AKT/STAT3/NF-κB pathway. Taken the present study results together, we can conclude that miR-140-5p may act as a novel target in microRNA-targeting anticancer strategy for the treatment of breast cancer. |
| format | Article |
| id | doaj-art-2d81e11ddb4a4102b7c20ccf08091a10 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-2d81e11ddb4a4102b7c20ccf08091a102025-02-03T06:37:53ZengSciendoActa Pharmaceutica1846-95582022-12-0172458759710.2478/acph-2022-0039MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cellsHou Lingli0Liu Qi1Zhao Ying2Yang Hongwei3Meng Qingying4Yu Fei5Department of Clinical Laboratory, Taihe Hospital, Affiliated Hospital of Hubei University of Medicine, Shiyan City Hubei Province, 442000, P.R.ChinaDepartment of Human Anatomy, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan City Hubei Province, 442000, P.R.ChinaDepartment of Clinical Laboratory, Taihe Hospital, Affiliated Hospital of Hubei University of Medicine, Shiyan City Hubei Province, 442000, P.R.ChinaDepartment of Clinical Laboratory, Taihe Hospital, Affiliated Hospital of Hubei University of Medicine, Shiyan City Hubei Province, 442000, P.R.ChinaDepartment of Medical Insurance, Yunxi People’s Hospital, Yunxi, Hubei Province 442600, P.R.ChinaDepartment of Clinical Laboratory, Yunxi People’s Hospital, Yunxi, Hubei Province442600, P.R.ChinaMicroRNA-140-5p (miR-140-5p) plays a pivotal role in human cancers. However, its role and molecular mechanisms in breast carcinoma are not fully explored. Using miR-140-5p transfected breast cancer cell line MDA-MB-231, several in vitro experiments were performed and described in this paper. They consist of the cell proliferation assay, wound healing assay, transwell assay, colony formation assays and qRTPCR. Expression levels of target proteins were determined using Western blotting. In addition, experiments on animal models were performed to study the possible role of miR-140-5p in tumorigenesis of breast carcinoma cells. The induction of experimental breast tumor in mice model was achieved through the incorporation of MDA-MB-231 tumor cells subcutaneously into the middle left side of the mice. The results showed that miR-140-5p up-regulation significantly suppresses proliferation, cellular invasion and migration of breast carcinoma cells. Furthermore, miR-140-5p up-regulation stops breast cancer cells at G0/G1 phase. The results of the animal model indicated that up-regulation of miR-140-5p suppresses its tumorigenic ability. Moreover, we also found that miR-140-5p up-regulation reduces the phosphorylation level of STAT3, p65, and AKT. In addition, miR-140-5p overexpression significantly decreases CDK2 expression while increasing E-cadherin expression level. These data revealed that miR-140-5p suppressed tumor progression of breast carcinoma cells through inhibition of the AKT/STAT3/NF-κB pathway. Taken the present study results together, we can conclude that miR-140-5p may act as a novel target in microRNA-targeting anticancer strategy for the treatment of breast cancer.https://doi.org/10.2478/acph-2022-0039breast cancermicrorna-140-5pcell proliferationtumor progressionakt/stat3/nf-κb |
| spellingShingle | Hou Lingli Liu Qi Zhao Ying Yang Hongwei Meng Qingying Yu Fei MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells Acta Pharmaceutica breast cancer microrna-140-5p cell proliferation tumor progression akt/stat3/nf-κb |
| title | MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells |
| title_full | MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells |
| title_fullStr | MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells |
| title_full_unstemmed | MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells |
| title_short | MicroRNA-140-5p inhibits cellular proliferation, migration and invasion by downregulating AKT/STAT3/NF-κB pathway in breast carcinoma cells |
| title_sort | microrna 140 5p inhibits cellular proliferation migration and invasion by downregulating akt stat3 nf κb pathway in breast carcinoma cells |
| topic | breast cancer microrna-140-5p cell proliferation tumor progression akt/stat3/nf-κb |
| url | https://doi.org/10.2478/acph-2022-0039 |
| work_keys_str_mv | AT houlingli microrna1405pinhibitscellularproliferationmigrationandinvasionbydownregulatingaktstat3nfkbpathwayinbreastcarcinomacells AT liuqi microrna1405pinhibitscellularproliferationmigrationandinvasionbydownregulatingaktstat3nfkbpathwayinbreastcarcinomacells AT zhaoying microrna1405pinhibitscellularproliferationmigrationandinvasionbydownregulatingaktstat3nfkbpathwayinbreastcarcinomacells AT yanghongwei microrna1405pinhibitscellularproliferationmigrationandinvasionbydownregulatingaktstat3nfkbpathwayinbreastcarcinomacells AT mengqingying microrna1405pinhibitscellularproliferationmigrationandinvasionbydownregulatingaktstat3nfkbpathwayinbreastcarcinomacells AT yufei microrna1405pinhibitscellularproliferationmigrationandinvasionbydownregulatingaktstat3nfkbpathwayinbreastcarcinomacells |