Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics
A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. The anti-chloramphenicol antibody (mAb) adopted in this work for direct immunoassay could bind to CAP specifically, with negligible...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/7131907 |
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| author | Qiyan Li Riran Zhu Jun Li Xiaobing Wang Lihua Xu Yanshen Li Peng Li |
| author_facet | Qiyan Li Riran Zhu Jun Li Xiaobing Wang Lihua Xu Yanshen Li Peng Li |
| author_sort | Qiyan Li |
| collection | DOAJ |
| description | A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. The anti-chloramphenicol antibody (mAb) adopted in this work for direct immunoassay could bind to CAP specifically, with negligible cross-reactivity (CR) (less than 0.01%) with most CAP analogues, including structurally related thiamphenicol (TAP) and florfenicol (FF). The limit of detection (LOD), measured by IC10, was 0.0021 ng mL−1. The detection range (IC20-IC80) was ranged from 0.00979 to 0.12026 ng mL−1. In spiked cosmetics samples, mean recoveries ranged from 82.7% to 99.6%, with intraday and interday variation less than 9.8 and 8.2%, respectively. Moreover, with the help of HRP-labeled anti-CAP mAb, the method could be processed in fast direct immunoreaction mode. This CL-ELISA method could be applied for specific, rapid, semiquantitative, and quantitative detection of CAP in cosmetics, facilitating the precise quality control of CAP contamination. |
| format | Article |
| id | doaj-art-2d54a6fc50154c1d812d0c2a5d86630e |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-2d54a6fc50154c1d812d0c2a5d86630e2025-02-03T01:31:32ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792019-01-01201910.1155/2019/71319077131907Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in CosmeticsQiyan Li0Riran Zhu1Jun Li2Xiaobing Wang3Lihua Xu4Yanshen Li5Peng Li6Health Food and Cosmetics Laboratory, Shandong Institute for Food and Drug Control, Jinan 250101, ChinaFaculty of Pharmaceutical Sciences, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250012, ChinaHealth Food and Cosmetics Laboratory, Shandong Institute for Food and Drug Control, Jinan 250101, ChinaHealth Food and Cosmetics Laboratory, Shandong Institute for Food and Drug Control, Jinan 250101, ChinaHealth Food and Cosmetics Laboratory, Shandong Institute for Food and Drug Control, Jinan 250101, ChinaCollege of Life Science, Yantai University, Yantai 264005, ChinaKey Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaA direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. The anti-chloramphenicol antibody (mAb) adopted in this work for direct immunoassay could bind to CAP specifically, with negligible cross-reactivity (CR) (less than 0.01%) with most CAP analogues, including structurally related thiamphenicol (TAP) and florfenicol (FF). The limit of detection (LOD), measured by IC10, was 0.0021 ng mL−1. The detection range (IC20-IC80) was ranged from 0.00979 to 0.12026 ng mL−1. In spiked cosmetics samples, mean recoveries ranged from 82.7% to 99.6%, with intraday and interday variation less than 9.8 and 8.2%, respectively. Moreover, with the help of HRP-labeled anti-CAP mAb, the method could be processed in fast direct immunoreaction mode. This CL-ELISA method could be applied for specific, rapid, semiquantitative, and quantitative detection of CAP in cosmetics, facilitating the precise quality control of CAP contamination.http://dx.doi.org/10.1155/2019/7131907 |
| spellingShingle | Qiyan Li Riran Zhu Jun Li Xiaobing Wang Lihua Xu Yanshen Li Peng Li Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics International Journal of Analytical Chemistry |
| title | Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics |
| title_full | Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics |
| title_fullStr | Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics |
| title_full_unstemmed | Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics |
| title_short | Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics |
| title_sort | highly specific chemiluminescence immunoassay for the determination of chloramphenicol in cosmetics |
| url | http://dx.doi.org/10.1155/2019/7131907 |
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