Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method
This study examines the Stearoyl-CoA desaturase (SCD) gene, which is essential for converting saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). The research aims to analyze the diversity of Single Nucleotide Polymorphism (SNP) g.10428C>T within the SCD gene using the PCR-RFLP m...
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Fakultas Peternakan Universitas Brawijaya
2025-04-01
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| Series: | Jurnal Ilmu-Ilmu Peternakan |
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| Online Access: | https://jiip.ub.ac.id/index.php/jiip/article/view/2981 |
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| author | Annisa Sarwi Ronny Rachman Noor Jakaria Jakaria |
| author_facet | Annisa Sarwi Ronny Rachman Noor Jakaria Jakaria |
| author_sort | Annisa Sarwi |
| collection | DOAJ |
| description | This study examines the Stearoyl-CoA desaturase (SCD) gene, which is essential for converting saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). The research aims to analyze the diversity of Single Nucleotide Polymorphism (SNP) g.10428C>T within the SCD gene using the PCR-RFLP method. The investigation involved 40 blood samples from male Bali cattle aged 1.5 to 3 years, sourced from the Banjarmasin Slaughterhouse (RPH) in South Kalimantan. The SNP g.10428C>T diversity in the SCD gene was analyzed using the PCR-RFLP technique with the HinfI restriction enzyme. Genotype frequencies, allele frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium were calculated using Popgen32. The relationship between fatty acid composition and the genotype of the SCD gene, as well as the fatty acids in Bali beef, was analyzed using a t-test. SCD|HinfI was found to have homozygous CC (0.03) and TT (0.85) and heterozygous CT (0.13) genotypes with a C allele frequency of 0.09 and T of 0.91. SNP g.10428C>T in the SCD gene was not significantly associated with fatty acid composition. |
| format | Article |
| id | doaj-art-2d2b3deadd2e4e3c96547bdaf883e9c3 |
| institution | Kabale University |
| issn | 0852-3681 2443-0765 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Fakultas Peternakan Universitas Brawijaya |
| record_format | Article |
| series | Jurnal Ilmu-Ilmu Peternakan |
| spelling | doaj-art-2d2b3deadd2e4e3c96547bdaf883e9c32025-08-20T03:33:14ZengFakultas Peternakan Universitas BrawijayaJurnal Ilmu-Ilmu Peternakan0852-36812443-07652025-04-013511910.21776/ub.jiip.2025.035.01.14410Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP MethodAnnisa Sarwi0https://orcid.org/0009-0002-9199-6036Ronny Rachman Noor1https://orcid.org/0000-0001-8795-2992Jakaria Jakaria2https://orcid.org/0000-0001-7921-8528Master’s Program, Animal Production Science and Technology Study Program, Postgraduate School, IPB University, 16680, IndonesiaDepartment of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.This study examines the Stearoyl-CoA desaturase (SCD) gene, which is essential for converting saturated fatty acids (SFA) into monounsaturated fatty acids (MUFA). The research aims to analyze the diversity of Single Nucleotide Polymorphism (SNP) g.10428C>T within the SCD gene using the PCR-RFLP method. The investigation involved 40 blood samples from male Bali cattle aged 1.5 to 3 years, sourced from the Banjarmasin Slaughterhouse (RPH) in South Kalimantan. The SNP g.10428C>T diversity in the SCD gene was analyzed using the PCR-RFLP technique with the HinfI restriction enzyme. Genotype frequencies, allele frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium were calculated using Popgen32. The relationship between fatty acid composition and the genotype of the SCD gene, as well as the fatty acids in Bali beef, was analyzed using a t-test. SCD|HinfI was found to have homozygous CC (0.03) and TT (0.85) and heterozygous CT (0.13) genotypes with a C allele frequency of 0.09 and T of 0.91. SNP g.10428C>T in the SCD gene was not significantly associated with fatty acid composition.https://jiip.ub.ac.id/index.php/jiip/article/view/2981bali cattlefatty acid compositionscd genesnps |
| spellingShingle | Annisa Sarwi Ronny Rachman Noor Jakaria Jakaria Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method Jurnal Ilmu-Ilmu Peternakan bali cattle fatty acid composition scd gene snps |
| title | Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method |
| title_full | Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method |
| title_fullStr | Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method |
| title_full_unstemmed | Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method |
| title_short | Stearoyl-CoA Desaturase (SCD) Gene Diversity and Its Association with Fatty Acids in Bali Cattle Using PCR-RFLP Method |
| title_sort | stearoyl coa desaturase scd gene diversity and its association with fatty acids in bali cattle using pcr rflp method |
| topic | bali cattle fatty acid composition scd gene snps |
| url | https://jiip.ub.ac.id/index.php/jiip/article/view/2981 |
| work_keys_str_mv | AT annisasarwi stearoylcoadesaturasescdgenediversityanditsassociationwithfattyacidsinbalicattleusingpcrrflpmethod AT ronnyrachmannoor stearoylcoadesaturasescdgenediversityanditsassociationwithfattyacidsinbalicattleusingpcrrflpmethod AT jakariajakaria stearoylcoadesaturasescdgenediversityanditsassociationwithfattyacidsinbalicattleusingpcrrflpmethod |