STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES
Objective: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and differentiate into various cell types. Their use in regenerative medicine is widespread today. However, for successful clinical application, the viability and potency of MSCs must be maintained during pretransplant preparati...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2022-02-01
|
| Series: | Sabiad |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/85B97F6980FD468A812B0CDC39F0E08E |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850194094000701440 |
|---|---|
| author | Mehmet Berker Emine Utlu Özen Fatma Öz Bağcı |
| author_facet | Mehmet Berker Emine Utlu Özen Fatma Öz Bağcı |
| author_sort | Mehmet Berker |
| collection | DOAJ |
| description | Objective: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and differentiate into various cell types. Their use in regenerative medicine is widespread today. However, for successful clinical application, the viability and potency of MSCs must be maintained during pretransplant preparation and transportation. The aim of this study (an area which has not been adequately researched), is to contribute to the literature on storage conditions, which is important for maintaining the effectiveness of MSCs. Material and Method: The viability of MSCs and CD73, CD90, CD105, CD19 surface antigens were evaluated in short-term in vitro storage in Phosphate Buffered Saline (PBS) and Serum Physiological solution at 4°C and room temperature (24°C). Conclusion: Although MSCs are similar in terms of viability and surface antigens in PBS and SF solution at 4°C and 24°C, higher results were obtained in cells kept in PBS at 4°C. Further research can be conducted by analyzing the differentiation, aging and proliferation capacities of MSCs under different environmental conditions. |
| format | Article |
| id | doaj-art-2d2b17ff01a44e93b9fa33a2ea4ea1bb |
| institution | OA Journals |
| issn | 2651-4060 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Sabiad |
| spelling | doaj-art-2d2b17ff01a44e93b9fa33a2ea4ea1bb2025-08-20T02:14:06ZengIstanbul University PressSabiad2651-40602022-02-0151202410.26650/JARHS2022-991485123456STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURESMehmet Berker0https://orcid.org/0000-0003-4755-023XEmine Utlu Özen1https://orcid.org/0000-0002-7397-8769Fatma Öz Bağcı2https://orcid.org/0000-0002-5507-9639İstanbul Üniversitesi, İstanbul, TürkiyeSağlık Bakanlığı, Ankara, TurkiyeNecmettin Erbakan Üniversitesi, Konya, TurkiyeObjective: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and differentiate into various cell types. Their use in regenerative medicine is widespread today. However, for successful clinical application, the viability and potency of MSCs must be maintained during pretransplant preparation and transportation. The aim of this study (an area which has not been adequately researched), is to contribute to the literature on storage conditions, which is important for maintaining the effectiveness of MSCs. Material and Method: The viability of MSCs and CD73, CD90, CD105, CD19 surface antigens were evaluated in short-term in vitro storage in Phosphate Buffered Saline (PBS) and Serum Physiological solution at 4°C and room temperature (24°C). Conclusion: Although MSCs are similar in terms of viability and surface antigens in PBS and SF solution at 4°C and 24°C, higher results were obtained in cells kept in PBS at 4°C. Further research can be conducted by analyzing the differentiation, aging and proliferation capacities of MSCs under different environmental conditions.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/85B97F6980FD468A812B0CDC39F0E08Emesenchymal stem cellflow cytometryvitality |
| spellingShingle | Mehmet Berker Emine Utlu Özen Fatma Öz Bağcı STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES Sabiad mesenchymal stem cell flow cytometry vitality |
| title | STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES |
| title_full | STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES |
| title_fullStr | STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES |
| title_full_unstemmed | STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES |
| title_short | STORAGE OF MESENCHYMAL STEM CELLS IN DIFFERENT SOLUTIONS AND TEMPERATURES |
| title_sort | storage of mesenchymal stem cells in different solutions and temperatures |
| topic | mesenchymal stem cell flow cytometry vitality |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/85B97F6980FD468A812B0CDC39F0E08E |
| work_keys_str_mv | AT mehmetberker storageofmesenchymalstemcellsindifferentsolutionsandtemperatures AT emineutluozen storageofmesenchymalstemcellsindifferentsolutionsandtemperatures AT fatmaozbagcı storageofmesenchymalstemcellsindifferentsolutionsandtemperatures |