Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles
Abstract Polymeric nanoparticles (NPs) are promising tools used for immunomodulation and drug delivery in various disease contexts. The interaction between NP surfaces and plasma-resident biomolecules results in the formation of a biomolecular corona, which varies patient-to-patient and as a functio...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56210-4 |
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| author | Jacob R. Shaw Nicholas Caprio Nhu Truong Mehari Weldemariam Anh Tran Nageswara Pilli Swarnima Pandey Jace W. Jones Maureen A. Kane Ryan M. Pearson |
| author_facet | Jacob R. Shaw Nicholas Caprio Nhu Truong Mehari Weldemariam Anh Tran Nageswara Pilli Swarnima Pandey Jace W. Jones Maureen A. Kane Ryan M. Pearson |
| author_sort | Jacob R. Shaw |
| collection | DOAJ |
| description | Abstract Polymeric nanoparticles (NPs) are promising tools used for immunomodulation and drug delivery in various disease contexts. The interaction between NP surfaces and plasma-resident biomolecules results in the formation of a biomolecular corona, which varies patient-to-patient and as a function of disease state. This study investigates how the progression of acute systemic inflammatory disease influences NP corona compositions and the corresponding effects on innate immune cell interactions, phenotypes, and cytokine responses. NP coronas alter cell associations in a disease-dependent manner, induce differential co-stimulatory and co-inhibitory molecule expression, and influence cytokine release. Integrated multi-omics analysis of proteomics, lipidomics, metabolomics, and cytokine datasets highlight a set of differentially enriched TLR4 ligands that correlate with dynamic NP corona-mediated immune activation. Pharmacological inhibition and genetic knockout studies validate that NP coronas mediate this response through TLR4/MyD88/NF-κB signaling. Our findings illuminate the personalized nature of corona formation under a dynamic inflammatory condition and its impact on NP-mediated immune activation profiles and inflammation, suggesting that disease progression-related alterations in plasma composition can manifest in the corona to cause unintended toxicity and altered therapeutic efficacy. |
| format | Article |
| id | doaj-art-2d0cb5d816a648a7bd1230657f058f77 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-2d0cb5d816a648a7bd1230657f058f772025-01-26T12:40:39ZengNature PortfolioNature Communications2041-17232025-01-0116111410.1038/s41467-025-56210-4Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profilesJacob R. Shaw0Nicholas Caprio1Nhu Truong2Mehari Weldemariam3Anh Tran4Nageswara Pilli5Swarnima Pandey6Jace W. Jones7Maureen A. Kane8Ryan M. Pearson9Department of Microbiology and Immunology, University of Maryland School of MedicineDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Pharmaceutical Sciences, University of Maryland School of PharmacyDepartment of Microbiology and Immunology, University of Maryland School of MedicineAbstract Polymeric nanoparticles (NPs) are promising tools used for immunomodulation and drug delivery in various disease contexts. The interaction between NP surfaces and plasma-resident biomolecules results in the formation of a biomolecular corona, which varies patient-to-patient and as a function of disease state. This study investigates how the progression of acute systemic inflammatory disease influences NP corona compositions and the corresponding effects on innate immune cell interactions, phenotypes, and cytokine responses. NP coronas alter cell associations in a disease-dependent manner, induce differential co-stimulatory and co-inhibitory molecule expression, and influence cytokine release. Integrated multi-omics analysis of proteomics, lipidomics, metabolomics, and cytokine datasets highlight a set of differentially enriched TLR4 ligands that correlate with dynamic NP corona-mediated immune activation. Pharmacological inhibition and genetic knockout studies validate that NP coronas mediate this response through TLR4/MyD88/NF-κB signaling. Our findings illuminate the personalized nature of corona formation under a dynamic inflammatory condition and its impact on NP-mediated immune activation profiles and inflammation, suggesting that disease progression-related alterations in plasma composition can manifest in the corona to cause unintended toxicity and altered therapeutic efficacy.https://doi.org/10.1038/s41467-025-56210-4 |
| spellingShingle | Jacob R. Shaw Nicholas Caprio Nhu Truong Mehari Weldemariam Anh Tran Nageswara Pilli Swarnima Pandey Jace W. Jones Maureen A. Kane Ryan M. Pearson Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles Nature Communications |
| title | Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles |
| title_full | Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles |
| title_fullStr | Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles |
| title_full_unstemmed | Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles |
| title_short | Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles |
| title_sort | inflammatory disease progression shapes nanoparticle biomolecular corona mediated immune activation profiles |
| url | https://doi.org/10.1038/s41467-025-56210-4 |
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