Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers
Abstract Background: Persimmon (Diospyros kaki L.) belongs to the Ebenaceae family, which includes six genera and about 400 species. This study evaluated the genetic diversity of 100 persimmon accessions from Hatay province, Türkiye using 42 morphological and pomological traits, along with inter sim...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86101-z |
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| author | Yazgan Tunç Canan Aydınlıoğlu Kadir Uğurtan Yılmaz Ali Khadivi Daya Shankar Mishra El Hassan Sakar Tomáš Vyhnánek |
| author_facet | Yazgan Tunç Canan Aydınlıoğlu Kadir Uğurtan Yılmaz Ali Khadivi Daya Shankar Mishra El Hassan Sakar Tomáš Vyhnánek |
| author_sort | Yazgan Tunç |
| collection | DOAJ |
| description | Abstract Background: Persimmon (Diospyros kaki L.) belongs to the Ebenaceae family, which includes six genera and about 400 species. This study evaluated the genetic diversity of 100 persimmon accessions from Hatay province, Türkiye using 42 morphological and pomological traits, along with inter simple sequence repeat (ISSR) markers and multivariate analysis. Results: Statistical analysis revealed significant differences among the accessions (ANOVA, p < 0.05). The coefficient of variation ranged from 19.24% for leaf length to 133.89% for fruit calyx groove end, with 97.62% of traits showing more than 20% variation. This indicates high genetic variability. Fruit weight, length, and diameter varied greatly, with strong positive correlations between fruit weight and other traits. Principal component analysis explained 72.42% of the total variance, while cluster analysis showed varying levels of similarity among accessions. ISSR analysis identified 139 bands, 128 of which were polymorphic. The similarity index ranged from 0.41 to 0.96. Notably, accessions ‘P78’, ‘P7’, ‘P48’, ‘P29’, ‘P44’, ‘P25’, ‘P5’, ‘P98’, ‘P80’, ‘P50’, ‘P37’, ‘P77’, ‘P57’, ‘P56’, ‘P41’, ‘P73’, ‘P39’, ‘P65’, ‘P72’, and ‘P61’ were identified as promising candidates for further study. Conclusions: This study demonstrates significant genetic diversity in persimmon accessions from Hatay. The high variability supports adaptability and resilience. Positive correlations among traits, especially fruit weight, are useful for breeding. ISSR markers highlighted valuable polymorphic bands, underlining the importance of local germplasm for developing resilient cultivars. Variations in growth vigor and ripening dates offer opportunities for customized cultivation practices, contributing to sustainable agriculture. |
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| id | doaj-art-2cf4fe81abf74949b0dfafaac7e0b56c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2cf4fe81abf74949b0dfafaac7e0b56c2025-01-19T12:17:37ZengNature PortfolioScientific Reports2045-23222025-01-0115111910.1038/s41598-025-86101-zDetermination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markersYazgan Tunç0Canan Aydınlıoğlu1Kadir Uğurtan Yılmaz2Ali Khadivi3Daya Shankar Mishra4El Hassan Sakar5Tomáš Vyhnánek6Republic of Türkiye, Ministry of Agriculture and Forestry, Hatay Olive Research Institute Directorate, General Directorate of Agricultural Research and PoliciesRepublic of Türkiye, Ministry of Agriculture and Forestry, Hatay Olive Research Institute Directorate, General Directorate of Agricultural Research and PoliciesDepartment of Horticulture, Faculty of Agriculture, Kahramanmaras Sutcu Imam UniversityDepartment of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Arak UniversityDepartment of Fruit Sciences, ICAR-CIAH RS Central Horticultural Experiment StationLaboratory of Biology, Ecology, and Health, FS, Abdelmalek Essaadi UniversityDepartment of Plant Biology, Faculty of AgriSciences, Mendel University in Brno, ZemědělskáAbstract Background: Persimmon (Diospyros kaki L.) belongs to the Ebenaceae family, which includes six genera and about 400 species. This study evaluated the genetic diversity of 100 persimmon accessions from Hatay province, Türkiye using 42 morphological and pomological traits, along with inter simple sequence repeat (ISSR) markers and multivariate analysis. Results: Statistical analysis revealed significant differences among the accessions (ANOVA, p < 0.05). The coefficient of variation ranged from 19.24% for leaf length to 133.89% for fruit calyx groove end, with 97.62% of traits showing more than 20% variation. This indicates high genetic variability. Fruit weight, length, and diameter varied greatly, with strong positive correlations between fruit weight and other traits. Principal component analysis explained 72.42% of the total variance, while cluster analysis showed varying levels of similarity among accessions. ISSR analysis identified 139 bands, 128 of which were polymorphic. The similarity index ranged from 0.41 to 0.96. Notably, accessions ‘P78’, ‘P7’, ‘P48’, ‘P29’, ‘P44’, ‘P25’, ‘P5’, ‘P98’, ‘P80’, ‘P50’, ‘P37’, ‘P77’, ‘P57’, ‘P56’, ‘P41’, ‘P73’, ‘P39’, ‘P65’, ‘P72’, and ‘P61’ were identified as promising candidates for further study. Conclusions: This study demonstrates significant genetic diversity in persimmon accessions from Hatay. The high variability supports adaptability and resilience. Positive correlations among traits, especially fruit weight, are useful for breeding. ISSR markers highlighted valuable polymorphic bands, underlining the importance of local germplasm for developing resilient cultivars. Variations in growth vigor and ripening dates offer opportunities for customized cultivation practices, contributing to sustainable agriculture.https://doi.org/10.1038/s41598-025-86101-zDiospyros kakiMultivariate analysisGenetic diversityInter simple sequence repeatsTürkiye |
| spellingShingle | Yazgan Tunç Canan Aydınlıoğlu Kadir Uğurtan Yılmaz Ali Khadivi Daya Shankar Mishra El Hassan Sakar Tomáš Vyhnánek Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers Scientific Reports Diospyros kaki Multivariate analysis Genetic diversity Inter simple sequence repeats Türkiye |
| title | Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers |
| title_full | Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers |
| title_fullStr | Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers |
| title_full_unstemmed | Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers |
| title_short | Determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers |
| title_sort | determination of genetic diversity in persimmon accessions using morphological and inter simple sequence repeat markers |
| topic | Diospyros kaki Multivariate analysis Genetic diversity Inter simple sequence repeats Türkiye |
| url | https://doi.org/10.1038/s41598-025-86101-z |
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