Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function.
Apart from the first family member, uncoupling protein 1 (UCP1), the functions of other UCPs (UCP2-UCP5) are still unknown. In analyzing our own results and those previously published by others, we have assumed that UCP's cellular expression pattern coincides with a specific cell metabolism and...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0088474 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850162275505143808 |
|---|---|
| author | Anne Rupprecht Dana Sittner Alina Smorodchenko Karolina E Hilse Justus Goyn Rudolf Moldzio Andrea E M Seiler Anja U Bräuer Elena E Pohl |
| author_facet | Anne Rupprecht Dana Sittner Alina Smorodchenko Karolina E Hilse Justus Goyn Rudolf Moldzio Andrea E M Seiler Anja U Bräuer Elena E Pohl |
| author_sort | Anne Rupprecht |
| collection | DOAJ |
| description | Apart from the first family member, uncoupling protein 1 (UCP1), the functions of other UCPs (UCP2-UCP5) are still unknown. In analyzing our own results and those previously published by others, we have assumed that UCP's cellular expression pattern coincides with a specific cell metabolism and changes if the latter is altered. To verify this hypothesis, we analyzed the expression of UCP1-5 in mouse embryonic stem cells before and after their differentiation to neurons. We have shown that only UCP2 is present in undifferentiated stem cells and it disappears simultaneously with the initiation of neuronal differentiation. In contrast, UCP4 is simultaneously up-regulated together with typical neuronal marker proteins TUJ-1 and NeuN during mESC differentiation in vitro as well as during murine brain development in vivo. Notably, several tested cell lines express UCP2, but not UCP4. In line with this finding, neuroblastoma cells that display metabolic features of tumor cells express UCP2, but not UCP4. UCP2's occurrence in cancer, immunological and stem cells indicates that UCP2 is present in cells with highly proliferative potential, which have a glycolytic type of metabolism as a common feature, whereas UCP4 is strongly associated with non-proliferative highly differentiated neuronal cells. |
| format | Article |
| id | doaj-art-2cc7e5101ac945e5933c7ab41eba3e5c |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-2cc7e5101ac945e5933c7ab41eba3e5c2025-08-20T02:22:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8847410.1371/journal.pone.0088474Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function.Anne RupprechtDana SittnerAlina SmorodchenkoKarolina E HilseJustus GoynRudolf MoldzioAndrea E M SeilerAnja U BräuerElena E PohlApart from the first family member, uncoupling protein 1 (UCP1), the functions of other UCPs (UCP2-UCP5) are still unknown. In analyzing our own results and those previously published by others, we have assumed that UCP's cellular expression pattern coincides with a specific cell metabolism and changes if the latter is altered. To verify this hypothesis, we analyzed the expression of UCP1-5 in mouse embryonic stem cells before and after their differentiation to neurons. We have shown that only UCP2 is present in undifferentiated stem cells and it disappears simultaneously with the initiation of neuronal differentiation. In contrast, UCP4 is simultaneously up-regulated together with typical neuronal marker proteins TUJ-1 and NeuN during mESC differentiation in vitro as well as during murine brain development in vivo. Notably, several tested cell lines express UCP2, but not UCP4. In line with this finding, neuroblastoma cells that display metabolic features of tumor cells express UCP2, but not UCP4. UCP2's occurrence in cancer, immunological and stem cells indicates that UCP2 is present in cells with highly proliferative potential, which have a glycolytic type of metabolism as a common feature, whereas UCP4 is strongly associated with non-proliferative highly differentiated neuronal cells.https://doi.org/10.1371/journal.pone.0088474 |
| spellingShingle | Anne Rupprecht Dana Sittner Alina Smorodchenko Karolina E Hilse Justus Goyn Rudolf Moldzio Andrea E M Seiler Anja U Bräuer Elena E Pohl Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. PLoS ONE |
| title | Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. |
| title_full | Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. |
| title_fullStr | Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. |
| title_full_unstemmed | Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. |
| title_short | Uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function. |
| title_sort | uncoupling protein 2 and 4 expression pattern during stem cell differentiation provides new insight into their putative function |
| url | https://doi.org/10.1371/journal.pone.0088474 |
| work_keys_str_mv | AT annerupprecht uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT danasittner uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT alinasmorodchenko uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT karolinaehilse uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT justusgoyn uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT rudolfmoldzio uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT andreaemseiler uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT anjaubrauer uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction AT elenaepohl uncouplingprotein2and4expressionpatternduringstemcelldifferentiationprovidesnewinsightintotheirputativefunction |