EGFR Amplification and Glioblastoma Stem-Like Cells
Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained in vitro using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However,...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/427518 |
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| _version_ | 1832561901381353472 |
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| author | Katrin Liffers Katrin Lamszus Alexander Schulte |
| author_facet | Katrin Liffers Katrin Lamszus Alexander Schulte |
| author_sort | Katrin Liffers |
| collection | DOAJ |
| description | Glioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained in vitro using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (EGFR) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained EGFR amplification could overcome the limitations of current in vitro model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different EGFR status in order to maintain EGFR-dependent intratumoral heterogeneity in vitro. Further, it will summarize the current knowledge about the impact of EGFR amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM. |
| format | Article |
| id | doaj-art-2cafcf71d9e14ba7b4a6e180ee98dc61 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-2cafcf71d9e14ba7b4a6e180ee98dc612025-02-03T01:23:52ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/427518427518EGFR Amplification and Glioblastoma Stem-Like CellsKatrin Liffers0Katrin Lamszus1Alexander Schulte2Laboratory for Brain Tumor Biology, Department of Neurosurgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyLaboratory for Brain Tumor Biology, Department of Neurosurgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyLaboratory for Brain Tumor Biology, Department of Neurosurgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, GermanyGlioblastoma (GBM), the most common malignant brain tumor in adults, contains a subpopulation of cells with a stem-like phenotype (GS-cells). GS-cells can be maintained in vitro using serum-free medium supplemented with epidermal growth factor, basic fibroblast growth factor-2, and heparin. However, this method does not conserve amplification of the Epidermal Growth Factor Receptor (EGFR) gene, which is present in over 50% of all newly diagnosed GBM cases. GS-cells with retained EGFR amplification could overcome the limitations of current in vitro model systems and contribute significantly to preclinical research on EGFR-targeted therapy. This review recapitulates recent methodological approaches to expand stem-like cells from GBM with different EGFR status in order to maintain EGFR-dependent intratumoral heterogeneity in vitro. Further, it will summarize the current knowledge about the impact of EGFR amplification and overexpression on the stem-like phenotype of GBM-derived GS-cells and different approaches to target the EGFR-dependent GS-cell compartment of GBM.http://dx.doi.org/10.1155/2015/427518 |
| spellingShingle | Katrin Liffers Katrin Lamszus Alexander Schulte EGFR Amplification and Glioblastoma Stem-Like Cells Stem Cells International |
| title | EGFR Amplification and Glioblastoma Stem-Like Cells |
| title_full | EGFR Amplification and Glioblastoma Stem-Like Cells |
| title_fullStr | EGFR Amplification and Glioblastoma Stem-Like Cells |
| title_full_unstemmed | EGFR Amplification and Glioblastoma Stem-Like Cells |
| title_short | EGFR Amplification and Glioblastoma Stem-Like Cells |
| title_sort | egfr amplification and glioblastoma stem like cells |
| url | http://dx.doi.org/10.1155/2015/427518 |
| work_keys_str_mv | AT katrinliffers egframplificationandglioblastomastemlikecells AT katrinlamszus egframplificationandglioblastomastemlikecells AT alexanderschulte egframplificationandglioblastomastemlikecells |