Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022
ObjectiveTo analyze the spatiotemporal clustering of anthrax cases in the Ningxia Hui Autonomous Region from 2019 to 2022 and to perform molecular typing of 14 strains of Bacillus anthracis (B. anthracis) isolated during the same period. MethodsData on anthrax cases reported in Ningxia Hui Autonomou...
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Editorial Office of Chinese Journal of Public Health
2024-10-01
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| Series: | Zhongguo gonggong weisheng |
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| Online Access: | https://www.zgggws.com/article/doi/10.11847/zgggws1144025 |
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| author | Ran XIAN Cong YANG Jianwei GAO Lei GAO Qian CHEN Yu ZHAO Wenhe KUAI Haohao FAN Xiaodi LI Jiangtao MA |
| author_facet | Ran XIAN Cong YANG Jianwei GAO Lei GAO Qian CHEN Yu ZHAO Wenhe KUAI Haohao FAN Xiaodi LI Jiangtao MA |
| author_sort | Ran XIAN |
| collection | DOAJ |
| description | ObjectiveTo analyze the spatiotemporal clustering of anthrax cases in the Ningxia Hui Autonomous Region from 2019 to 2022 and to perform molecular typing of 14 strains of Bacillus anthracis (B. anthracis) isolated during the same period. MethodsData on anthrax cases reported in Ningxia Hui Autonomous Region (Ningxia) from 2019 to 2022 were collected, and spatiotemporal cluster analysis was performed using SaTScan software with districts and counties as units. A total of 14 strains of B. anthracis isolated from local anthrax cases from 2019 to 2021 were subjected to molecular typing analysis using canonical single nucleotide polymorphisms (CanSNPs), twenty-five marker multilocus variable number tandem repeat analysis (MLVA-25), and single nucleotide repeat (SNR-4) methods. ResultsA total of 80 anthrax cases, all of which were cutaneous anthrax, were reported in Ningxia during the 3-year period. SaTScan spatiotemporal cluster analysis revealed a spatiotemporal cluster in north-central Ningxia from 2021 to 2022, with a primary cluster in Yongning county [P < 0.01, relative risk (RR) = 13.83] and secondary clusters in Lingwu city and Litong district (P = 0.022, RR = 3.47). The genetic relationships of the 14 B. anthracis strains isolated during the same period were defined as: A.Br 001/002 subgroup, 6 different MLVA genotypes within A3.b gene cluster, of which 3 common genotypes were defined as 2, 3, and 5 PHANA genotypes, respectively, by SNR-4 analysis; SNR-4 analysis showed 12 SNR genotypes. ConclusionThe incidence of anthrax in Ningxia showed an increasing trend from 2019 to 2022. One of the reasons for the formation of spatiotemporal clusters of anthrax in 2021 – 2022 may be the formation and development of natural foci. Molecular typing revealed that the genetic relationship of the strains isolated in Ningxia from 2019 to 2022 was relatively homogeneous, with abundant genetic diversity within the same genetic relationship. |
| format | Article |
| id | doaj-art-2cae7e80c8d640dca06ab71e17a10632 |
| institution | Kabale University |
| issn | 1001-0580 |
| language | zho |
| publishDate | 2024-10-01 |
| publisher | Editorial Office of Chinese Journal of Public Health |
| record_format | Article |
| series | Zhongguo gonggong weisheng |
| spelling | doaj-art-2cae7e80c8d640dca06ab71e17a106322025-01-23T05:11:54ZzhoEditorial Office of Chinese Journal of Public HealthZhongguo gonggong weisheng1001-05802024-10-0140101235124310.11847/zgggws11440251144025Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022Ran XIAN0Cong YANG1Jianwei GAO2Lei GAO3Qian CHEN4Yu ZHAO5Wenhe KUAI6Haohao FAN7Xiaodi LI8Jiangtao MA9Ningxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Ningxia Medical University, Yinchuan 750001, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaNingxia Center for Disease Control and Prevention, Yinchuan 750004, ChinaObjectiveTo analyze the spatiotemporal clustering of anthrax cases in the Ningxia Hui Autonomous Region from 2019 to 2022 and to perform molecular typing of 14 strains of Bacillus anthracis (B. anthracis) isolated during the same period. MethodsData on anthrax cases reported in Ningxia Hui Autonomous Region (Ningxia) from 2019 to 2022 were collected, and spatiotemporal cluster analysis was performed using SaTScan software with districts and counties as units. A total of 14 strains of B. anthracis isolated from local anthrax cases from 2019 to 2021 were subjected to molecular typing analysis using canonical single nucleotide polymorphisms (CanSNPs), twenty-five marker multilocus variable number tandem repeat analysis (MLVA-25), and single nucleotide repeat (SNR-4) methods. ResultsA total of 80 anthrax cases, all of which were cutaneous anthrax, were reported in Ningxia during the 3-year period. SaTScan spatiotemporal cluster analysis revealed a spatiotemporal cluster in north-central Ningxia from 2021 to 2022, with a primary cluster in Yongning county [P < 0.01, relative risk (RR) = 13.83] and secondary clusters in Lingwu city and Litong district (P = 0.022, RR = 3.47). The genetic relationships of the 14 B. anthracis strains isolated during the same period were defined as: A.Br 001/002 subgroup, 6 different MLVA genotypes within A3.b gene cluster, of which 3 common genotypes were defined as 2, 3, and 5 PHANA genotypes, respectively, by SNR-4 analysis; SNR-4 analysis showed 12 SNR genotypes. ConclusionThe incidence of anthrax in Ningxia showed an increasing trend from 2019 to 2022. One of the reasons for the formation of spatiotemporal clusters of anthrax in 2021 – 2022 may be the formation and development of natural foci. Molecular typing revealed that the genetic relationship of the strains isolated in Ningxia from 2019 to 2022 was relatively homogeneous, with abundant genetic diversity within the same genetic relationship.https://www.zgggws.com/article/doi/10.11847/zgggws1144025anthraxtwenty-five marker multi-locus variable number tandem repeat analysiscanonical single nucleotide polymorphismssingle nucleotide repeatspatiotemporal cluster analysisningxia hui autonomous region |
| spellingShingle | Ran XIAN Cong YANG Jianwei GAO Lei GAO Qian CHEN Yu ZHAO Wenhe KUAI Haohao FAN Xiaodi LI Jiangtao MA Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022 Zhongguo gonggong weisheng anthrax twenty-five marker multi-locus variable number tandem repeat analysis canonical single nucleotide polymorphisms single nucleotide repeat spatiotemporal cluster analysis ningxia hui autonomous region |
| title | Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022 |
| title_full | Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022 |
| title_fullStr | Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022 |
| title_full_unstemmed | Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022 |
| title_short | Spatiotemporal clustering of anthrax and molecular typing of Bacillus anthracis in Ningxia Hui Autonomous Region, 2019 – 2022 |
| title_sort | spatiotemporal clustering of anthrax and molecular typing of bacillus anthracis in ningxia hui autonomous region 2019 2022 |
| topic | anthrax twenty-five marker multi-locus variable number tandem repeat analysis canonical single nucleotide polymorphisms single nucleotide repeat spatiotemporal cluster analysis ningxia hui autonomous region |
| url | https://www.zgggws.com/article/doi/10.11847/zgggws1144025 |
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