Comparison of Different Promoter Methylation Assays in Breast Cancer
Background: Promoter hypermethylation has emerged as a promising cancer biomarker. Currently, a large variety of quantitative and non-quantitative techniques is used to measure methylation in clinical specimens. Here we directly compared three commonly used methylation assays and assessed the influe...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-CLO-2010-0542 |
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| _version_ | 1832567713334034432 |
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| author | Karijn P. M. Suijkerbuijk Xiaojuan Pan Elsken van der Wall Paul J. van Diest Marc Vooijs |
| author_facet | Karijn P. M. Suijkerbuijk Xiaojuan Pan Elsken van der Wall Paul J. van Diest Marc Vooijs |
| author_sort | Karijn P. M. Suijkerbuijk |
| collection | DOAJ |
| description | Background: Promoter hypermethylation has emerged as a promising cancer biomarker. Currently, a large variety of quantitative and non-quantitative techniques is used to measure methylation in clinical specimens. Here we directly compared three commonly used methylation assays and assessed the influence of tissue fixation, target sequence location and the amount of DNA on their performance. |
| format | Article |
| id | doaj-art-2c986b62a5ae4cf38fb49051f98060e5 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-2c986b62a5ae4cf38fb49051f98060e52025-02-03T01:00:40ZengWileyAnalytical Cellular Pathology2210-71772210-71852010-01-01333-413314110.3233/ACP-CLO-2010-0542Comparison of Different Promoter Methylation Assays in Breast CancerKarijn P. M. Suijkerbuijk0Xiaojuan Pan1Elsken van der Wall2Paul J. van Diest3Marc Vooijs4Department of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The NetherlandsDepartment of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The NetherlandsDepartment of Radiation Oncology (Maastro Lab), GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, The NetherlandsDepartment of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The NetherlandsDepartment of Pathology, University Medical Center Utrecht Cancer Center, Utrecht, The NetherlandsBackground: Promoter hypermethylation has emerged as a promising cancer biomarker. Currently, a large variety of quantitative and non-quantitative techniques is used to measure methylation in clinical specimens. Here we directly compared three commonly used methylation assays and assessed the influence of tissue fixation, target sequence location and the amount of DNA on their performance.http://dx.doi.org/10.3233/ACP-CLO-2010-0542 |
| spellingShingle | Karijn P. M. Suijkerbuijk Xiaojuan Pan Elsken van der Wall Paul J. van Diest Marc Vooijs Comparison of Different Promoter Methylation Assays in Breast Cancer Analytical Cellular Pathology |
| title | Comparison of Different Promoter Methylation Assays in Breast Cancer |
| title_full | Comparison of Different Promoter Methylation Assays in Breast Cancer |
| title_fullStr | Comparison of Different Promoter Methylation Assays in Breast Cancer |
| title_full_unstemmed | Comparison of Different Promoter Methylation Assays in Breast Cancer |
| title_short | Comparison of Different Promoter Methylation Assays in Breast Cancer |
| title_sort | comparison of different promoter methylation assays in breast cancer |
| url | http://dx.doi.org/10.3233/ACP-CLO-2010-0542 |
| work_keys_str_mv | AT karijnpmsuijkerbuijk comparisonofdifferentpromotermethylationassaysinbreastcancer AT xiaojuanpan comparisonofdifferentpromotermethylationassaysinbreastcancer AT elskenvanderwall comparisonofdifferentpromotermethylationassaysinbreastcancer AT pauljvandiest comparisonofdifferentpromotermethylationassaysinbreastcancer AT marcvooijs comparisonofdifferentpromotermethylationassaysinbreastcancer |