Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus
Abstract Vibrio parahaemolyticus is pathogenic to both humans and marine animals. Antimicrobial-resistant (AMR) bacteria have been reported to cause mortalities in shrimp, with phage therapy presenting an alternative and eco-friendly biocontrol strategy for controlling bacterial diseases. Therefore,...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-85513-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585833631186944 |
|---|---|
| author | Te-Ken Hsu Yi-Yin Chen Shiao-Wen Li Hui-Yu Shih Hsin-Yiu Chou Jeff Chia-Kai Hsu Han-Ching Wang Li-Li Chen |
| author_facet | Te-Ken Hsu Yi-Yin Chen Shiao-Wen Li Hui-Yu Shih Hsin-Yiu Chou Jeff Chia-Kai Hsu Han-Ching Wang Li-Li Chen |
| author_sort | Te-Ken Hsu |
| collection | DOAJ |
| description | Abstract Vibrio parahaemolyticus is pathogenic to both humans and marine animals. Antimicrobial-resistant (AMR) bacteria have been reported to cause mortalities in shrimp, with phage therapy presenting an alternative and eco-friendly biocontrol strategy for controlling bacterial diseases. Therefore, this study aimed to isolate and characterize phages for their applicability in lysing Vibrio parahaemolyticus. A novel phage vB_VpaS_BP15 (BP15) belonged to the subfamily Queuovirinae with an icosahedral head measuring 69.11 ± 5.38 nm in length and 65.40 ± 6.89 nm in width, and a non-contractile sheathed tail measuring 139.81 ± 14.79 nm. The one-step growth curve indicated a latent period of 30 min and a burst size of 120 PFUs per cell. Phage BP15 exhibited tolerance to a range of temperatures and pH values. Infection dynamic curves demonstrated that BP15 was highly effective against BCRC12959 at MOIs ranging from 0.01 to 10; even at a low multiplicity of infection (MOI) of 0.001, BP15 still caused growth retention. Phage BP15 possessed a circular double-stranded DNA of 59,584 bp with a G + C content of 46.7% and lacked tRNA genes, virulence genes, and lysogeny genes. These findings highlight the promising potential of phage BP15 as a biocontrol agent against Vibrio parahaemolyticus in Taiwan. |
| format | Article |
| id | doaj-art-2c89309265144a0a9697069ec2493a03 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2c89309265144a0a9697069ec2493a032025-01-26T12:26:05ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85513-1Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticusTe-Ken Hsu0Yi-Yin Chen1Shiao-Wen Li2Hui-Yu Shih3Hsin-Yiu Chou4Jeff Chia-Kai Hsu5Han-Ching Wang6Li-Li Chen7Institute of Marine Biology, National Taiwan Ocean UniversityGraduate Institute of Immunology, College of Medicine, National Taiwan UniversityDepartment of Life Sciences, National University of KaohsiungInstitute of Marine Biology, National Taiwan Ocean UniversityDepartment of Aquaculture, National Taiwan Ocean UniversityInstitute of Marine Biology, National Taiwan Ocean UniversityDepartment of Biotechnology and Bioindustry Sciences, College of Biosciences and Biotechnology, National Cheng Kung UniversityInstitute of Marine Biology, National Taiwan Ocean UniversityAbstract Vibrio parahaemolyticus is pathogenic to both humans and marine animals. Antimicrobial-resistant (AMR) bacteria have been reported to cause mortalities in shrimp, with phage therapy presenting an alternative and eco-friendly biocontrol strategy for controlling bacterial diseases. Therefore, this study aimed to isolate and characterize phages for their applicability in lysing Vibrio parahaemolyticus. A novel phage vB_VpaS_BP15 (BP15) belonged to the subfamily Queuovirinae with an icosahedral head measuring 69.11 ± 5.38 nm in length and 65.40 ± 6.89 nm in width, and a non-contractile sheathed tail measuring 139.81 ± 14.79 nm. The one-step growth curve indicated a latent period of 30 min and a burst size of 120 PFUs per cell. Phage BP15 exhibited tolerance to a range of temperatures and pH values. Infection dynamic curves demonstrated that BP15 was highly effective against BCRC12959 at MOIs ranging from 0.01 to 10; even at a low multiplicity of infection (MOI) of 0.001, BP15 still caused growth retention. Phage BP15 possessed a circular double-stranded DNA of 59,584 bp with a G + C content of 46.7% and lacked tRNA genes, virulence genes, and lysogeny genes. These findings highlight the promising potential of phage BP15 as a biocontrol agent against Vibrio parahaemolyticus in Taiwan.https://doi.org/10.1038/s41598-025-85513-1Vibrio parahaemolyticusPhagePhage therapyVibriosisAquaculture |
| spellingShingle | Te-Ken Hsu Yi-Yin Chen Shiao-Wen Li Hui-Yu Shih Hsin-Yiu Chou Jeff Chia-Kai Hsu Han-Ching Wang Li-Li Chen Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus Scientific Reports Vibrio parahaemolyticus Phage Phage therapy Vibriosis Aquaculture |
| title | Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus |
| title_full | Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus |
| title_fullStr | Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus |
| title_full_unstemmed | Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus |
| title_short | Characterization and genome analysis of a novel phage BP15 infecting Vibrio parahaemolyticus |
| title_sort | characterization and genome analysis of a novel phage bp15 infecting vibrio parahaemolyticus |
| topic | Vibrio parahaemolyticus Phage Phage therapy Vibriosis Aquaculture |
| url | https://doi.org/10.1038/s41598-025-85513-1 |
| work_keys_str_mv | AT tekenhsu characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT yiyinchen characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT shiaowenli characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT huiyushih characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT hsinyiuchou characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT jeffchiakaihsu characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT hanchingwang characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus AT lilichen characterizationandgenomeanalysisofanovelphagebp15infectingvibrioparahaemolyticus |