Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination
Seed germination is critical for crop production and is sensitive to abiotic stresses. Considering that nitric oxide (NO) has been shown to ameliorate the negative effects of abiotic stress in several plants, we investigated the effect and mechanisms of exogenous NO in wheat seed germination under F...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325002118 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861241749176320 |
|---|---|
| author | Shuqian Shi Zhe Zhang Rongrong Ma Yihui Tao Ziling Wang Yingli Yang |
| author_facet | Shuqian Shi Zhe Zhang Rongrong Ma Yihui Tao Ziling Wang Yingli Yang |
| author_sort | Shuqian Shi |
| collection | DOAJ |
| description | Seed germination is critical for crop production and is sensitive to abiotic stresses. Considering that nitric oxide (NO) has been shown to ameliorate the negative effects of abiotic stress in several plants, we investigated the effect and mechanisms of exogenous NO in wheat seed germination under Fe stress, focusing on oxidative stress. Specifically, seeds of the wheat cultivar ‘Ningchun 4’ were treated with 1 mM FeCl3 (Fe) or with the NO donor sodium nitroprusside (SNP) and/or the specific NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO). Changes in endogenous NO production, Fe content, starch mobilisation, and antioxidant enzyme activity were examined at the early (24 h) and late (72 h) germination stages. Notably, Fe and PTIO treatments reduced the germination potential, germination rate, germination index, and vitality index of wheat seeds during the early and late germination stages. Additionally, Fe and PTIO treatments reduced endogenous NO levels, increased starch, sucrose, and soluble sugar contents, induced oxidative stress, suppressed antioxidant enzymes, and downregulated nitrate reductase, NO synthase, and amylase activities. However, SNP treatment ameliorated Fe stress-induced inhibition of seed germination, increased NO levels, upregulated starch degradation, and improved the antioxidant system and reactive oxygen species scavenging. Conclusively, exogenous NO treatment alleviates the inhibitory effects of Fe on wheat seed germination by enhancing endogenous NO synthesis, promoting starch decomposition, and alleviating oxidative damage. |
| format | Article |
| id | doaj-art-2c5f1644d5c4452697e2c59268a54247 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-2c5f1644d5c4452697e2c59268a542472025-02-10T04:33:30ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117875Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germinationShuqian Shi0Zhe Zhang1Rongrong Ma2Yihui Tao3Ziling Wang4Yingli Yang5College of Life Science, Northwest Normal University, Lanzhou 730070, PR ChinaCollege of Life Science, Northwest Normal University, Lanzhou 730070, PR ChinaCollege of Life Science, Northwest Normal University, Lanzhou 730070, PR ChinaCollege of Life Science, Northwest Normal University, Lanzhou 730070, PR ChinaCollege of Life Science, Northwest Normal University, Lanzhou 730070, PR ChinaCorrespondence to: School of Life Science, Northwest Normal University, Lanzhou, Gansu 730070, PR China.; College of Life Science, Northwest Normal University, Lanzhou 730070, PR ChinaSeed germination is critical for crop production and is sensitive to abiotic stresses. Considering that nitric oxide (NO) has been shown to ameliorate the negative effects of abiotic stress in several plants, we investigated the effect and mechanisms of exogenous NO in wheat seed germination under Fe stress, focusing on oxidative stress. Specifically, seeds of the wheat cultivar ‘Ningchun 4’ were treated with 1 mM FeCl3 (Fe) or with the NO donor sodium nitroprusside (SNP) and/or the specific NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO). Changes in endogenous NO production, Fe content, starch mobilisation, and antioxidant enzyme activity were examined at the early (24 h) and late (72 h) germination stages. Notably, Fe and PTIO treatments reduced the germination potential, germination rate, germination index, and vitality index of wheat seeds during the early and late germination stages. Additionally, Fe and PTIO treatments reduced endogenous NO levels, increased starch, sucrose, and soluble sugar contents, induced oxidative stress, suppressed antioxidant enzymes, and downregulated nitrate reductase, NO synthase, and amylase activities. However, SNP treatment ameliorated Fe stress-induced inhibition of seed germination, increased NO levels, upregulated starch degradation, and improved the antioxidant system and reactive oxygen species scavenging. Conclusively, exogenous NO treatment alleviates the inhibitory effects of Fe on wheat seed germination by enhancing endogenous NO synthesis, promoting starch decomposition, and alleviating oxidative damage.http://www.sciencedirect.com/science/article/pii/S0147651325002118Antioxidant responseIron stressNitric oxideSeed germinationStorage material mobilization |
| spellingShingle | Shuqian Shi Zhe Zhang Rongrong Ma Yihui Tao Ziling Wang Yingli Yang Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination Ecotoxicology and Environmental Safety Antioxidant response Iron stress Nitric oxide Seed germination Storage material mobilization |
| title | Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination |
| title_full | Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination |
| title_fullStr | Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination |
| title_full_unstemmed | Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination |
| title_short | Exogenous nitric oxide relieves iron-inhibitory effect on wheat seed germination |
| title_sort | exogenous nitric oxide relieves iron inhibitory effect on wheat seed germination |
| topic | Antioxidant response Iron stress Nitric oxide Seed germination Storage material mobilization |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325002118 |
| work_keys_str_mv | AT shuqianshi exogenousnitricoxiderelievesironinhibitoryeffectonwheatseedgermination AT zhezhang exogenousnitricoxiderelievesironinhibitoryeffectonwheatseedgermination AT rongrongma exogenousnitricoxiderelievesironinhibitoryeffectonwheatseedgermination AT yihuitao exogenousnitricoxiderelievesironinhibitoryeffectonwheatseedgermination AT zilingwang exogenousnitricoxiderelievesironinhibitoryeffectonwheatseedgermination AT yingliyang exogenousnitricoxiderelievesironinhibitoryeffectonwheatseedgermination |