Protocol to quantitatively assess glycolysis and related carbon metabolic fluxes using stable isotope tracing in Crabtree-positive yeasts

Protocol to quantitatively assess glycolysis and related carbon metabolic fluxes using stable isotope tracing in Crabtree-positive yeasts

Summary: Crabtree-positive yeasts rapidly consume glucose via glycolysis, making it difficult to experimentally estimate their actual glycolytic rate or flux. We present a stable isotope labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based protocol to quantitatively estimate...

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Bibliographic Details
Main Authors: Shreyas Niphadkar, Sreesa Sreedharan, Vineeth Vengayil, Sunil Laxman
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
metabolism
metabolomics
model organisms
systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001923
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