Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens
Abstract Molecular diagnosis limitations, including complex treatment processes, low cost‐effectiveness, and operator‐dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-03-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202411849 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850119612157394944 |
|---|---|
| author | Sung Eun Seo Kyung Ho Kim Seo Jin Kim Kyong‐Cheol Ko Woo‐Keun Kim Kyoung G. Lee Oh Seok Kwon |
| author_facet | Sung Eun Seo Kyung Ho Kim Seo Jin Kim Kyong‐Cheol Ko Woo‐Keun Kim Kyoung G. Lee Oh Seok Kwon |
| author_sort | Sung Eun Seo |
| collection | DOAJ |
| description | Abstract Molecular diagnosis limitations, including complex treatment processes, low cost‐effectiveness, and operator‐dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array‐based film is presented for superior photothermal energy conversion. Magnifying the metal film surface‐to‐volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time. Plasmonic energy‐based ultrafast gene amplification and facile confirmation methodology offer a rapid disease discrimination platform for high‐throughput multiplexed diagnosis. The superior performance of the gold nanopillar arrayed film is demonstrated by measuring the amount of pathogen (Vibrio cholerae) with a sensitivity of 101 cfu mL−1 in 5.5 min. The newly engineered gold nanopillar arrayed film can be utilized to diagnose universal pathogens to achieve an increasingly successful complete cure. |
| format | Article |
| id | doaj-art-2c3caa8efbdb4eaf96deb80b2a77c119 |
| institution | OA Journals |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-2c3caa8efbdb4eaf96deb80b2a77c1192025-08-20T02:35:35ZengWileyAdvanced Science2198-38442025-03-01129n/an/a10.1002/advs.202411849Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of PathogensSung Eun Seo0Kyung Ho Kim1Seo Jin Kim2Kyong‐Cheol Ko3Woo‐Keun Kim4Kyoung G. Lee5Oh Seok Kwon6SKKU Advanced Institute of Nanotechnology (SAINT) Sungkyunkwan University Suwon 16419 South KoreaSKKU Advanced Institute of Nanotechnology (SAINT) Sungkyunkwan University Suwon 16419 South KoreaSKKU Advanced Institute of Nanotechnology (SAINT) Sungkyunkwan University Suwon 16419 South KoreaKorea Preclinical Evaluation Center Korea Research Institute of Bioscience and Biotechnology (KRIBB) 125 Gwahak‐ro, Yuseong‐gu Daejeon 34141 South KoreaDepartment of Predictive Toxicology Korea Institute of Toxicology 141 Gajeong‐ro, Yuseong‐gu Daejeon 34114 South KoreaCenter for NanoBio Development National NanoFab Center (NNFC) Daejeon 34141 South KoreaSKKU Advanced Institute of Nanotechnology (SAINT) Sungkyunkwan University Suwon 16419 South KoreaAbstract Molecular diagnosis limitations, including complex treatment processes, low cost‐effectiveness, and operator‐dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array‐based film is presented for superior photothermal energy conversion. Magnifying the metal film surface‐to‐volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time. Plasmonic energy‐based ultrafast gene amplification and facile confirmation methodology offer a rapid disease discrimination platform for high‐throughput multiplexed diagnosis. The superior performance of the gold nanopillar arrayed film is demonstrated by measuring the amount of pathogen (Vibrio cholerae) with a sensitivity of 101 cfu mL−1 in 5.5 min. The newly engineered gold nanopillar arrayed film can be utilized to diagnose universal pathogens to achieve an increasingly successful complete cure.https://doi.org/10.1002/advs.202411849environmental monitoringGold nanopillar arraypathogen diagnosisphotothermal energy conversionplasmonic PCR |
| spellingShingle | Sung Eun Seo Kyung Ho Kim Seo Jin Kim Kyong‐Cheol Ko Woo‐Keun Kim Kyoung G. Lee Oh Seok Kwon Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens Advanced Science environmental monitoring Gold nanopillar array pathogen diagnosis photothermal energy conversion plasmonic PCR |
| title | Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens |
| title_full | Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens |
| title_fullStr | Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens |
| title_full_unstemmed | Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens |
| title_short | Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens |
| title_sort | plasmon driven gold nanopillar multiarrayed gene amplification methodology for the high throughput discrimination of pathogens |
| topic | environmental monitoring Gold nanopillar array pathogen diagnosis photothermal energy conversion plasmonic PCR |
| url | https://doi.org/10.1002/advs.202411849 |
| work_keys_str_mv | AT sungeunseo plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens AT kyunghokim plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens AT seojinkim plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens AT kyongcheolko plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens AT wookeunkim plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens AT kyoungglee plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens AT ohseokkwon plasmondrivengoldnanopillarmultiarrayedgeneamplificationmethodologyforthehighthroughputdiscriminationofpathogens |