Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression
Introduction. Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (n-RHLS) in an animal model of periodontitis...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2024/5015893 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546184882814976 |
|---|---|
| author | Ira Arundina Theresia Indah Budhy Aqsa Sjuhada Oki Meircurius Dwi Condro Surboyo Arvind Babu Rajendra Santosh null Sidarningsih Indeswati Diyatri Tytania Rahmaputry Arya Pradana Mohammad Iqbal Azzahra Salsabila Adira Moelyanto |
| author_facet | Ira Arundina Theresia Indah Budhy Aqsa Sjuhada Oki Meircurius Dwi Condro Surboyo Arvind Babu Rajendra Santosh null Sidarningsih Indeswati Diyatri Tytania Rahmaputry Arya Pradana Mohammad Iqbal Azzahra Salsabila Adira Moelyanto |
| author_sort | Ira Arundina |
| collection | DOAJ |
| description | Introduction. Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (n-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kβ (RANK), and receptor activator of nuclear factor-kβ ligand (RANKL). Methods. Twenty-eight male Wistar rats were inoculated with 109 CFU/ml of Porphyromonas gingivalis in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment. Results. OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (p<0.05). In contrast, RANK expression did not show significant differences compared to the control groups (p>0.05). Conclusion. Nanostructured liquid smoke derived from rice husk nanoparticles (n-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health. |
| format | Article |
| id | doaj-art-2c3bd30999d64c9b85437ff9d90d99ca |
| institution | Kabale University |
| issn | 1687-8795 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-2c3bd30999d64c9b85437ff9d90d99ca2025-02-03T07:23:39ZengWileyInternational Journal of Biomaterials1687-87952024-01-01202410.1155/2024/5015893Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL ExpressionIra Arundina0Theresia Indah Budhy1Aqsa Sjuhada Oki2Meircurius Dwi Condro Surboyo3Arvind Babu Rajendra Santosh4null Sidarningsih5Indeswati Diyatri6Tytania Rahmaputry7Arya Pradana8Mohammad Iqbal9Azzahra Salsabila Adira Moelyanto10Department of Oral BiologyDepartment of Oral Pathology and MaxillofacialDepartment of Oral BiologyDepartment of Oral MedicineSchool of DentistryDepartment of Oral BiologyDepartment of Oral BiologySchool of DentistryFaculty of Dental MedicineFaculty of Dental MedicineDental Health ScienceIntroduction. Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (n-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kβ (RANK), and receptor activator of nuclear factor-kβ ligand (RANKL). Methods. Twenty-eight male Wistar rats were inoculated with 109 CFU/ml of Porphyromonas gingivalis in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment. Results. OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (p<0.05). In contrast, RANK expression did not show significant differences compared to the control groups (p>0.05). Conclusion. Nanostructured liquid smoke derived from rice husk nanoparticles (n-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health.http://dx.doi.org/10.1155/2024/5015893 |
| spellingShingle | Ira Arundina Theresia Indah Budhy Aqsa Sjuhada Oki Meircurius Dwi Condro Surboyo Arvind Babu Rajendra Santosh null Sidarningsih Indeswati Diyatri Tytania Rahmaputry Arya Pradana Mohammad Iqbal Azzahra Salsabila Adira Moelyanto Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression International Journal of Biomaterials |
| title | Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression |
| title_full | Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression |
| title_fullStr | Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression |
| title_full_unstemmed | Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression |
| title_short | Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression |
| title_sort | nanoparticle based rice husk liquid smoke as periodontitis therapy through opg rank and rankl expression |
| url | http://dx.doi.org/10.1155/2024/5015893 |
| work_keys_str_mv | AT iraarundina nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT theresiaindahbudhy nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT aqsasjuhadaoki nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT meircuriusdwicondrosurboyo nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT arvindbaburajendrasantosh nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT nullsidarningsih nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT indeswatidiyatri nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT tytaniarahmaputry nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT aryapradana nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT mohammadiqbal nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression AT azzahrasalsabilaadiramoelyanto nanoparticlebasedricehuskliquidsmokeasperiodontitistherapythroughopgrankandranklexpression |