Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach
In many industrial areas such as in food, pharmaceutical, cosmetic, printing, and textile, the use of synthetic dyes has been integral with products such as azo dye, anthrax, and dyestuffs. As such, these industries produce a lot of waste by-products that could contaminate the environment. Bioremedi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/8296239 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559957075034112 |
|---|---|
| author | Fatin Nur Sufinas Shuib Ahmad Husaini Azham Zulkharnain Hairul Azman Roslan Tay Meng Guan |
| author_facet | Fatin Nur Sufinas Shuib Ahmad Husaini Azham Zulkharnain Hairul Azman Roslan Tay Meng Guan |
| author_sort | Fatin Nur Sufinas Shuib |
| collection | DOAJ |
| description | In many industrial areas such as in food, pharmaceutical, cosmetic, printing, and textile, the use of synthetic dyes has been integral with products such as azo dye, anthrax, and dyestuffs. As such, these industries produce a lot of waste by-products that could contaminate the environment. Bioremediation, therefore, has become an important emerging technology due to its cost-sustainable, effective, natural approach to cleaning up contaminated groundwater and soil via the use of microorganisms. The use of microorganisms in bioremediation requires the optimisation of parameters used in cultivating the organism. Thus the aim of the work was to assess the degradation of Remazol Brilliant Blue R (RBBR) dye on soil using Plackett-Burman design by the basidiomycete, M. cladophyllus UMAS MS8. Biodegradation analyses were carried out on a soil spiked with RBBR and supplemented with rice husk as the fungus growth enhancer. A two-level Plackett-Burman design was used to screen the medium components for the effects on the decolourization of RBBR. For the analysis, eleven variables were selected and from these four parameters, dye concentration, yeast extract concentration, inoculum size, and incubation time, were found to be most effective to degrade RBBR with up to 91% RBBR removal in soil after 15 days. |
| format | Article |
| id | doaj-art-2bf967deb5164d83b8d8cb8712836958 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2bf967deb5164d83b8d8cb87128369582025-02-03T01:28:46ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/82962398296239Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical ApproachFatin Nur Sufinas Shuib0Ahmad Husaini1Azham Zulkharnain2Hairul Azman Roslan3Tay Meng Guan4Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, MalaysiaDepartment of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, MalaysiaDepartment of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, MalaysiaDepartment of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, MalaysiaDepartment of Chemistry, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, MalaysiaIn many industrial areas such as in food, pharmaceutical, cosmetic, printing, and textile, the use of synthetic dyes has been integral with products such as azo dye, anthrax, and dyestuffs. As such, these industries produce a lot of waste by-products that could contaminate the environment. Bioremediation, therefore, has become an important emerging technology due to its cost-sustainable, effective, natural approach to cleaning up contaminated groundwater and soil via the use of microorganisms. The use of microorganisms in bioremediation requires the optimisation of parameters used in cultivating the organism. Thus the aim of the work was to assess the degradation of Remazol Brilliant Blue R (RBBR) dye on soil using Plackett-Burman design by the basidiomycete, M. cladophyllus UMAS MS8. Biodegradation analyses were carried out on a soil spiked with RBBR and supplemented with rice husk as the fungus growth enhancer. A two-level Plackett-Burman design was used to screen the medium components for the effects on the decolourization of RBBR. For the analysis, eleven variables were selected and from these four parameters, dye concentration, yeast extract concentration, inoculum size, and incubation time, were found to be most effective to degrade RBBR with up to 91% RBBR removal in soil after 15 days.http://dx.doi.org/10.1155/2016/8296239 |
| spellingShingle | Fatin Nur Sufinas Shuib Ahmad Husaini Azham Zulkharnain Hairul Azman Roslan Tay Meng Guan Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach The Scientific World Journal |
| title | Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach |
| title_full | Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach |
| title_fullStr | Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach |
| title_full_unstemmed | Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach |
| title_short | Optimization of Physiochemical Parameters during Bioremediation of Synthetic Dye by Marasmius cladophyllus UMAS MS8 Using Statistical Approach |
| title_sort | optimization of physiochemical parameters during bioremediation of synthetic dye by marasmius cladophyllus umas ms8 using statistical approach |
| url | http://dx.doi.org/10.1155/2016/8296239 |
| work_keys_str_mv | AT fatinnursufinasshuib optimizationofphysiochemicalparametersduringbioremediationofsyntheticdyebymarasmiuscladophyllusumasms8usingstatisticalapproach AT ahmadhusaini optimizationofphysiochemicalparametersduringbioremediationofsyntheticdyebymarasmiuscladophyllusumasms8usingstatisticalapproach AT azhamzulkharnain optimizationofphysiochemicalparametersduringbioremediationofsyntheticdyebymarasmiuscladophyllusumasms8usingstatisticalapproach AT hairulazmanroslan optimizationofphysiochemicalparametersduringbioremediationofsyntheticdyebymarasmiuscladophyllusumasms8usingstatisticalapproach AT taymengguan optimizationofphysiochemicalparametersduringbioremediationofsyntheticdyebymarasmiuscladophyllusumasms8usingstatisticalapproach |