Effects of Different Temperature-Time Combinations on Protein Absorption Characteristics of Sous-Vide Cooked Beef Tendon

In this study, marinated beef tendon was subjected to sous-vide cooking under different combinations of temperature and time. Traditional high-temperature stewing was used as a control. The cooked meat was digested in vitro and the digest was added to an in vitro absorption model based on Caco-2 cel...

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Bibliographic Details
Main Author: LI Tianyi, SUN Jian
Format: Article
Language:English
Published: China Food Publishing Company 2024-12-01
Series:Shipin Kexue
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Online Access:https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-021.pdf
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Summary:In this study, marinated beef tendon was subjected to sous-vide cooking under different combinations of temperature and time. Traditional high-temperature stewing was used as a control. The cooked meat was digested in vitro and the digest was added to an in vitro absorption model based on Caco-2 cells to simulate the absorption process. The effects of different temperature-time combinations on the protein absorption of sous-vide cooked beef tendon were determined by measuring the protein absorption products. The results showed that sous-vide processing increased the absorption of free amino acids and peptides especially those with small molecular mass of 300-500 Da. Sous-vide cooking at 60 ℃ favored the absorption of myosin and actin, while sous-vide cooking at 80 ℃ favored the absorption of collagen. Compared with the other treatment groups, sous-vide cooking at 60 ℃ for 8 h resulted in the occurrence of significantly more essential amino acids and antioxidant amino acids as well as more small-molecular-mass peptides and specific bioactive peptides in the absorbed products, showing higher nutritional levels.
ISSN:1002-6630