Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities
Sequential extraction using a cold maceration method and column chromatographic separation of the roots Kniphofia insignis headed to the isolation of three anthraquinones: one monomeric anthraquinone (1) and two dimeric anthraquinones (2 and 3). It was further purified by Sephadex LH-20 and recrysta...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/6661116 |
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| author | Asnakew Amare Tadesse Negera Abdissa Ayana Dele Abdissa Keneni |
| author_facet | Asnakew Amare Tadesse Negera Abdissa Ayana Dele Abdissa Keneni |
| author_sort | Asnakew Amare Tadesse |
| collection | DOAJ |
| description | Sequential extraction using a cold maceration method and column chromatographic separation of the roots Kniphofia insignis headed to the isolation of three anthraquinones: one monomeric anthraquinone (1) and two dimeric anthraquinones (2 and 3). It was further purified by Sephadex LH-20 and recrystallized. The structures of these compounds were established based on the spectroscopic analyses including NMR (1H-NMR and 13C-NMR and infrared) and comparison with reported literatures. In an in vitro antimicrobial assay of the crude extracts, the isolated compounds were made against four bacterial strains (S. aureus ATCC 25923, B. subtilis ATCC 6633, E. coli ATCC 35218, and P. aeruginosa ATCC 27853) and Fusarium spp. fungal strain. In the crude extracts of chloroform, substantial antimicrobial activity was seen with the highest activity against B. subtilis (16 mm) and E. coli (22 mm). Meanwhile, compound 1 has a better zone of inhibition with 14 mm against P. aeruginosa, whereas compound 2 showed better activity (13 mm) against Fusarium spp. fungal strain. |
| format | Article |
| id | doaj-art-2bece7eaf4a849aca2bdb4382bcd4b27 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-2bece7eaf4a849aca2bdb4382bcd4b272025-02-03T06:43:48ZengWileyJournal of Chemistry2090-90632090-90712021-01-01202110.1155/2021/66611166661116Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial ActivitiesAsnakew Amare Tadesse0Negera Abdissa Ayana1Dele Abdissa Keneni2Department of Chemistry, College of Natural Sciences, Woldia University, P.O. Box 400, Woldia, EthiopiaDepartment of Chemistry, College of Natural Sciences, Jimma University, P.O. Box 378, Jimma, EthiopiaDepartment of Chemistry, College of Natural Sciences, Jimma University, P.O. Box 378, Jimma, EthiopiaSequential extraction using a cold maceration method and column chromatographic separation of the roots Kniphofia insignis headed to the isolation of three anthraquinones: one monomeric anthraquinone (1) and two dimeric anthraquinones (2 and 3). It was further purified by Sephadex LH-20 and recrystallized. The structures of these compounds were established based on the spectroscopic analyses including NMR (1H-NMR and 13C-NMR and infrared) and comparison with reported literatures. In an in vitro antimicrobial assay of the crude extracts, the isolated compounds were made against four bacterial strains (S. aureus ATCC 25923, B. subtilis ATCC 6633, E. coli ATCC 35218, and P. aeruginosa ATCC 27853) and Fusarium spp. fungal strain. In the crude extracts of chloroform, substantial antimicrobial activity was seen with the highest activity against B. subtilis (16 mm) and E. coli (22 mm). Meanwhile, compound 1 has a better zone of inhibition with 14 mm against P. aeruginosa, whereas compound 2 showed better activity (13 mm) against Fusarium spp. fungal strain.http://dx.doi.org/10.1155/2021/6661116 |
| spellingShingle | Asnakew Amare Tadesse Negera Abdissa Ayana Dele Abdissa Keneni Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities Journal of Chemistry |
| title | Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities |
| title_full | Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities |
| title_fullStr | Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities |
| title_full_unstemmed | Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities |
| title_short | Anthraquinones from the Roots of Kniphofia insignis and Evaluation of Their Antimicrobial Activities |
| title_sort | anthraquinones from the roots of kniphofia insignis and evaluation of their antimicrobial activities |
| url | http://dx.doi.org/10.1155/2021/6661116 |
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