Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis
Basal, lipopolysaccharide (LPS) and silica-stimulated prostaglandin (PG) production were compared between peripheral blood mononuclear cells (PBMNC) from UC patients and healthy subjects (HS). Basal and LPS-stimulated PBMNC PGI2, but not PGE2, production was greater in UC. LPS stimulated both PGE2 a...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2000-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350020002903 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548593586667520 |
|---|---|
| author | Neville A. Punchard John Cason Jonathan Mullins Chaman Chander Richard P. H. Thompson |
| author_facet | Neville A. Punchard John Cason Jonathan Mullins Chaman Chander Richard P. H. Thompson |
| author_sort | Neville A. Punchard |
| collection | DOAJ |
| description | Basal, lipopolysaccharide (LPS) and silica-stimulated prostaglandin (PG) production were compared between peripheral blood mononuclear cells (PBMNC) from UC patients and healthy subjects (HS). Basal and LPS-stimulated PBMNC PGI2, but not PGE2, production was greater in UC. LPS stimulated both PGE2 and PGI2 by PBMNC from HS and UC patients. Silica stimulated production of both PGs by cells from HS but only
PGE2 by cells from UC patients. The differences in responses to silica and LPS may result from differences in activation of NFκB or, alternatively, prior sensitisation to one of these agents. That PBMNC PGE2 production is not increased in UC, as it is in Crohn’s disease, suggests that there are differences in PBMNC behaviour between these two diseases. |
| format | Article |
| id | doaj-art-2be02d107824418ba899c491e1634db5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2be02d107824418ba899c491e1634db52025-02-03T06:13:28ZengWileyMediators of Inflammation0962-93511466-18612000-01-0193-418919110.1080/09629350020002903Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitisNeville A. Punchard0John Cason1Jonathan Mullins2Chaman Chander3Richard P. H. Thompson4Department of Biology and Health Science, Faculty of Science, Technology &Design, University of Luton, Park Square, Luton LU1 3JU, UKGastrointestinal Laboratory, The Rayne Institute, St Thomas′ Hospital, London SE1 7EH, UKDepartment of Biology and Health Science, Faculty of Science, Technology &Design, University of Luton, Park Square, Luton LU1 3JU, UKDepartment of Biology and Health Science, Faculty of Science, Technology &Design, University of Luton, Park Square, Luton LU1 3JU, UKGastrointestinal Laboratory, The Rayne Institute, St Thomas′ Hospital, London SE1 7EH, UKBasal, lipopolysaccharide (LPS) and silica-stimulated prostaglandin (PG) production were compared between peripheral blood mononuclear cells (PBMNC) from UC patients and healthy subjects (HS). Basal and LPS-stimulated PBMNC PGI2, but not PGE2, production was greater in UC. LPS stimulated both PGE2 and PGI2 by PBMNC from HS and UC patients. Silica stimulated production of both PGs by cells from HS but only PGE2 by cells from UC patients. The differences in responses to silica and LPS may result from differences in activation of NFκB or, alternatively, prior sensitisation to one of these agents. That PBMNC PGE2 production is not increased in UC, as it is in Crohn’s disease, suggests that there are differences in PBMNC behaviour between these two diseases.http://dx.doi.org/10.1080/09629350020002903 |
| spellingShingle | Neville A. Punchard John Cason Jonathan Mullins Chaman Chander Richard P. H. Thompson Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis Mediators of Inflammation |
| title | Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis |
| title_full | Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis |
| title_fullStr | Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis |
| title_full_unstemmed | Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis |
| title_short | Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis |
| title_sort | lipopolysaccharide and silica stimulated mononuclear cell prostaglandin production in ulcerative colitis |
| url | http://dx.doi.org/10.1080/09629350020002903 |
| work_keys_str_mv | AT nevilleapunchard lipopolysaccharideandsilicastimulatedmononuclearcellprostaglandinproductioninulcerativecolitis AT johncason lipopolysaccharideandsilicastimulatedmononuclearcellprostaglandinproductioninulcerativecolitis AT jonathanmullins lipopolysaccharideandsilicastimulatedmononuclearcellprostaglandinproductioninulcerativecolitis AT chamanchander lipopolysaccharideandsilicastimulatedmononuclearcellprostaglandinproductioninulcerativecolitis AT richardphthompson lipopolysaccharideandsilicastimulatedmononuclearcellprostaglandinproductioninulcerativecolitis |