The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice
Glucosinolates are chemically stable compounds that exhibit biological activity in the body following hydrolysis catalyzed by the enzyme myrosinase. While existing <i>in vitro</i> and <i>in vivo</i> studies suggest that the hydrolysis products of glucosinolates predominantly...
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2025-01-01
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| author | Nikola Ferara Vedran Balta Domagoj Đikić Dyana Odeh Ana Mojsović-Ćuić Lana Feher Turković Dario Dilber Anđelo Beletić Irena Landeka Jurčević Ivana Šola |
| author_facet | Nikola Ferara Vedran Balta Domagoj Đikić Dyana Odeh Ana Mojsović-Ćuić Lana Feher Turković Dario Dilber Anđelo Beletić Irena Landeka Jurčević Ivana Šola |
| author_sort | Nikola Ferara |
| collection | DOAJ |
| description | Glucosinolates are chemically stable compounds that exhibit biological activity in the body following hydrolysis catalyzed by the enzyme myrosinase. While existing <i>in vitro</i> and <i>in vivo</i> studies suggest that the hydrolysis products of glucosinolates predominantly exert beneficial effects in both human and animal organisms, some studies have found that the excessive consumption of glucosinolates may lead to toxic and anti-nutritional effects. Given that glucosinolates are primarily ingested in the human diet through dietary supplements and commercially available cruciferous vegetables, we investigated the <i>in vivo</i> effects of the glucosinolate sinigrin on molecular markers in the myocardia of healthy Swiss mice. This study aims to elucidate whether sinigrin induces positive or negative physiological effects in mammals following consumption. The alterations in myocardial parameters were assessed by measuring metabolic, inflammatory, structural, and antioxidant markers. Our findings revealed that subchronic exposure to sinigrin in the myocardia of female mice resulted in a significant increase (<i>p</i> ≤ 0.05) in the levels of the myokine irisin, matrix metalloproteinases (MMP-2, MMP-9), catalase (CAT), and total glutathione (tGSH), alongside a marked decrease (<i>p</i> ≤ 0.05) in the levels of atrial natriuretic peptide (ANP), compared to the control group consisting of both female and male mice. These results suggest that the hydrolysis products of sinigrin may exert a potentially toxic effect on the myocardial tissue of female mice and possess the capability to modulate transcription factors <i>in vivo</i> in a sex-dependent manner. This observation calls for further investigation into the mechanisms regulating the actions of glucosinolate hydrolysis products, their interactions with sex hormones, and the determination of permissible intake levels associated with both beneficial and adverse outcomes. |
| format | Article |
| id | doaj-art-2bdc5be2913a423ea76683629733a644 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-2bdc5be2913a423ea76683629733a6442025-01-24T13:33:18ZengMDPI AGFoods2304-81582025-01-0114232710.3390/foods14020327The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss MiceNikola Ferara0Vedran Balta1Domagoj Đikić2Dyana Odeh3Ana Mojsović-Ćuić4Lana Feher Turković5Dario Dilber6Anđelo Beletić7Irena Landeka Jurčević8Ivana Šola9Department of Dermatology and Venereology, Sestre Milosrdnice University Hospital Centre, Vinogradska cesta 29, 10000 Zagreb, CroatiaFaculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, CroatiaFaculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, CroatiaFaculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, CroatiaSchool of Applied Health Sciences, University of Zagreb, Mlinarska cesta 38, 10000 Zagreb, CroatiaSchool of Applied Health Sciences, University of Zagreb, Mlinarska cesta 38, 10000 Zagreb, CroatiaDepartment of Cardiology, Thalassotherapia Opatija, Maršala Tita 188, 51410 Opatija, CroatiaLaboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CroatiaFaculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, CroatiaFaculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, CroatiaGlucosinolates are chemically stable compounds that exhibit biological activity in the body following hydrolysis catalyzed by the enzyme myrosinase. While existing <i>in vitro</i> and <i>in vivo</i> studies suggest that the hydrolysis products of glucosinolates predominantly exert beneficial effects in both human and animal organisms, some studies have found that the excessive consumption of glucosinolates may lead to toxic and anti-nutritional effects. Given that glucosinolates are primarily ingested in the human diet through dietary supplements and commercially available cruciferous vegetables, we investigated the <i>in vivo</i> effects of the glucosinolate sinigrin on molecular markers in the myocardia of healthy Swiss mice. This study aims to elucidate whether sinigrin induces positive or negative physiological effects in mammals following consumption. The alterations in myocardial parameters were assessed by measuring metabolic, inflammatory, structural, and antioxidant markers. Our findings revealed that subchronic exposure to sinigrin in the myocardia of female mice resulted in a significant increase (<i>p</i> ≤ 0.05) in the levels of the myokine irisin, matrix metalloproteinases (MMP-2, MMP-9), catalase (CAT), and total glutathione (tGSH), alongside a marked decrease (<i>p</i> ≤ 0.05) in the levels of atrial natriuretic peptide (ANP), compared to the control group consisting of both female and male mice. These results suggest that the hydrolysis products of sinigrin may exert a potentially toxic effect on the myocardial tissue of female mice and possess the capability to modulate transcription factors <i>in vivo</i> in a sex-dependent manner. This observation calls for further investigation into the mechanisms regulating the actions of glucosinolate hydrolysis products, their interactions with sex hormones, and the determination of permissible intake levels associated with both beneficial and adverse outcomes.https://www.mdpi.com/2304-8158/14/2/327glucosinolatesinigrin (allyl-glucosinolate)myocardgendercardiac physiology |
| spellingShingle | Nikola Ferara Vedran Balta Domagoj Đikić Dyana Odeh Ana Mojsović-Ćuić Lana Feher Turković Dario Dilber Anđelo Beletić Irena Landeka Jurčević Ivana Šola The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice Foods glucosinolate sinigrin (allyl-glucosinolate) myocard gender cardiac physiology |
| title | The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice |
| title_full | The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice |
| title_fullStr | The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice |
| title_full_unstemmed | The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice |
| title_short | The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice |
| title_sort | effect of the glucosinolate sinigrin on alterations in molecular biomarkers of the myocardium in swiss mice |
| topic | glucosinolate sinigrin (allyl-glucosinolate) myocard gender cardiac physiology |
| url | https://www.mdpi.com/2304-8158/14/2/327 |
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