Characterization of the Merkel Cell Carcinoma miRNome
MicroRNAs have been implicated in various skin cancers, including melanoma, squamous cell carcinoma, and basal cell carcinoma; however, the expression of microRNAs and their role in Merkel cell carcinoma (MCC) have yet to be explored in depth. To identify microRNAs specific to MCC (MCC-miRs), next-g...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Skin Cancer |
| Online Access: | http://dx.doi.org/10.1155/2014/289548 |
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| _version_ | 1832551656301002752 |
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| author | Matthew S. Ning Annette S. Kim Nripesh Prasad Shawn E. Levy Huiqiu Zhang Thomas Andl |
| author_facet | Matthew S. Ning Annette S. Kim Nripesh Prasad Shawn E. Levy Huiqiu Zhang Thomas Andl |
| author_sort | Matthew S. Ning |
| collection | DOAJ |
| description | MicroRNAs have been implicated in various skin cancers, including melanoma, squamous cell carcinoma, and basal cell carcinoma; however, the expression of microRNAs and their role in Merkel cell carcinoma (MCC) have yet to be explored in depth. To identify microRNAs specific to MCC (MCC-miRs), next-generation sequencing (NGS) of small RNA libraries was performed on different tissue samples including MCCs, other cutaneous tumors, and normal skin. Comparison of the profiles identified several microRNAs upregulated and downregulated in MCC. For validation, their expression was measured via qRT-PCR in a larger group of MCC and in a comparison group of non-MCC cutaneous tumors and normal skin. Eight microRNAs were upregulated in MCC: miR-502-3p, miR-9, miR-7, miR-340, miR-182, miR-190b, miR-873, and miR-183. Three microRNAs were downregulated: miR-3170, miR-125b, and miR-374c. Many of these MCC-miRs, the miR-183/182/96a cistron in particular, have connections to tumorigenic pathways implicated in MCC pathogenesis. In situ hybridization confirmed that the highly expressed MCC-miR, miR-182, is localized within tumor cells. Furthermore, NGS and qRT-PCR reveal that several of these MCC-miRs are highly expressed in the patient-derived MCC cell line, MS-1. These data indicate that we have identified a set of MCC-miRs with important implications for MCC research. |
| format | Article |
| id | doaj-art-2bc582de855d48b78b89fe30c974b437 |
| institution | Kabale University |
| issn | 2090-2905 2090-2913 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Skin Cancer |
| spelling | doaj-art-2bc582de855d48b78b89fe30c974b4372025-02-03T06:00:53ZengWileyJournal of Skin Cancer2090-29052090-29132014-01-01201410.1155/2014/289548289548Characterization of the Merkel Cell Carcinoma miRNomeMatthew S. Ning0Annette S. Kim1Nripesh Prasad2Shawn E. Levy3Huiqiu Zhang4Thomas Andl5Division of Dermatology, Department of Medicine, Vanderbilt University Medical Center, Medical Center North, Room A2310A, 1161 21st Avenue South, Nashville, TN 37232-2600, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232-2600, USADepartment of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL 35805, USAHudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USAOrioR Lab, LLC, Rockville, MD 20852, USADivision of Dermatology, Department of Medicine, Vanderbilt University Medical Center, Medical Center North, Room A2310A, 1161 21st Avenue South, Nashville, TN 37232-2600, USAMicroRNAs have been implicated in various skin cancers, including melanoma, squamous cell carcinoma, and basal cell carcinoma; however, the expression of microRNAs and their role in Merkel cell carcinoma (MCC) have yet to be explored in depth. To identify microRNAs specific to MCC (MCC-miRs), next-generation sequencing (NGS) of small RNA libraries was performed on different tissue samples including MCCs, other cutaneous tumors, and normal skin. Comparison of the profiles identified several microRNAs upregulated and downregulated in MCC. For validation, their expression was measured via qRT-PCR in a larger group of MCC and in a comparison group of non-MCC cutaneous tumors and normal skin. Eight microRNAs were upregulated in MCC: miR-502-3p, miR-9, miR-7, miR-340, miR-182, miR-190b, miR-873, and miR-183. Three microRNAs were downregulated: miR-3170, miR-125b, and miR-374c. Many of these MCC-miRs, the miR-183/182/96a cistron in particular, have connections to tumorigenic pathways implicated in MCC pathogenesis. In situ hybridization confirmed that the highly expressed MCC-miR, miR-182, is localized within tumor cells. Furthermore, NGS and qRT-PCR reveal that several of these MCC-miRs are highly expressed in the patient-derived MCC cell line, MS-1. These data indicate that we have identified a set of MCC-miRs with important implications for MCC research.http://dx.doi.org/10.1155/2014/289548 |
| spellingShingle | Matthew S. Ning Annette S. Kim Nripesh Prasad Shawn E. Levy Huiqiu Zhang Thomas Andl Characterization of the Merkel Cell Carcinoma miRNome Journal of Skin Cancer |
| title | Characterization of the Merkel Cell Carcinoma miRNome |
| title_full | Characterization of the Merkel Cell Carcinoma miRNome |
| title_fullStr | Characterization of the Merkel Cell Carcinoma miRNome |
| title_full_unstemmed | Characterization of the Merkel Cell Carcinoma miRNome |
| title_short | Characterization of the Merkel Cell Carcinoma miRNome |
| title_sort | characterization of the merkel cell carcinoma mirnome |
| url | http://dx.doi.org/10.1155/2014/289548 |
| work_keys_str_mv | AT matthewsning characterizationofthemerkelcellcarcinomamirnome AT annetteskim characterizationofthemerkelcellcarcinomamirnome AT nripeshprasad characterizationofthemerkelcellcarcinomamirnome AT shawnelevy characterizationofthemerkelcellcarcinomamirnome AT huiqiuzhang characterizationofthemerkelcellcarcinomamirnome AT thomasandl characterizationofthemerkelcellcarcinomamirnome |