Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study
Cancer treatment with high LET heavy ion beam, especially, carbon ion beam (12C), is becoming very popular over conventional radiotherapy like low LET gamma or X-ray. Combination of Poly(ADP-ribose) polymerase (PARP) inhibitor with xenotoxic drugs or conventional radiation (gamma or X-ray) is the ne...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2014/438030 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559402602725376 |
|---|---|
| author | Atanu Ghorai Nitai P. Bhattacharyya Asitikantha Sarma Utpal Ghosh |
| author_facet | Atanu Ghorai Nitai P. Bhattacharyya Asitikantha Sarma Utpal Ghosh |
| author_sort | Atanu Ghorai |
| collection | DOAJ |
| description | Cancer treatment with high LET heavy ion beam, especially, carbon ion beam (12C), is becoming very popular over conventional radiotherapy like low LET gamma or X-ray. Combination of Poly(ADP-ribose) polymerase (PARP) inhibitor with xenotoxic drugs or conventional radiation (gamma or X-ray) is the newer approach for cancer therapy. The aim of our study was to compare the radiosensitivity and induction of apoptosis by high LET 12C and low LET gamma radiation in HeLa and PARP-1 knocked down cells. We did comet assay to detect DNA breaks, clonogenic survival assay, and cell cycle analysis to measure recovery after DNA damage. We measured apoptotic parameters like nuclear fragmentation and caspase-3 activation. DNA damage, cell killing, and induction of apoptosis were significantly higher for 12C than gamma radiation in HeLa. Cell killing and apoptosis were further elevated upon knocking down of PARP-1. Both 12C and gamma induced G2/M arrest although the 12C had greater effect. Unlike the gamma, 12C irradiation affects DNA replication as detected by S-phase delay in cell cycle analysis. So, we conclude that high LET 12C has greater potential over low LET gamma radiation in killing cells and radiosensitization upon PARP-1 inhibition was several folds greater for 12C than gamma. |
| format | Article |
| id | doaj-art-2bc05eeff9bc419291f086bc6146930d |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-2bc05eeff9bc419291f086bc6146930d2025-02-03T01:30:01ZengWileyScientifica2090-908X2014-01-01201410.1155/2014/438030438030Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative StudyAtanu Ghorai0Nitai P. Bhattacharyya1Asitikantha Sarma2Utpal Ghosh3Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741235, IndiaCrystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064, IndiaInter-University Accelerator Center (IUAC), Aruna Asaf Ali Marg, New Delhi 110067, IndiaDepartment of Biochemistry & Biophysics, University of Kalyani, Kalyani 741235, IndiaCancer treatment with high LET heavy ion beam, especially, carbon ion beam (12C), is becoming very popular over conventional radiotherapy like low LET gamma or X-ray. Combination of Poly(ADP-ribose) polymerase (PARP) inhibitor with xenotoxic drugs or conventional radiation (gamma or X-ray) is the newer approach for cancer therapy. The aim of our study was to compare the radiosensitivity and induction of apoptosis by high LET 12C and low LET gamma radiation in HeLa and PARP-1 knocked down cells. We did comet assay to detect DNA breaks, clonogenic survival assay, and cell cycle analysis to measure recovery after DNA damage. We measured apoptotic parameters like nuclear fragmentation and caspase-3 activation. DNA damage, cell killing, and induction of apoptosis were significantly higher for 12C than gamma radiation in HeLa. Cell killing and apoptosis were further elevated upon knocking down of PARP-1. Both 12C and gamma induced G2/M arrest although the 12C had greater effect. Unlike the gamma, 12C irradiation affects DNA replication as detected by S-phase delay in cell cycle analysis. So, we conclude that high LET 12C has greater potential over low LET gamma radiation in killing cells and radiosensitization upon PARP-1 inhibition was several folds greater for 12C than gamma.http://dx.doi.org/10.1155/2014/438030 |
| spellingShingle | Atanu Ghorai Nitai P. Bhattacharyya Asitikantha Sarma Utpal Ghosh Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study Scientifica |
| title | Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study |
| title_full | Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study |
| title_fullStr | Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study |
| title_full_unstemmed | Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study |
| title_short | Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study |
| title_sort | radiosensitivity and induction of apoptosis by high let carbon ion beam and low let gamma radiation a comparative study |
| url | http://dx.doi.org/10.1155/2014/438030 |
| work_keys_str_mv | AT atanughorai radiosensitivityandinductionofapoptosisbyhighletcarbonionbeamandlowletgammaradiationacomparativestudy AT nitaipbhattacharyya radiosensitivityandinductionofapoptosisbyhighletcarbonionbeamandlowletgammaradiationacomparativestudy AT asitikanthasarma radiosensitivityandinductionofapoptosisbyhighletcarbonionbeamandlowletgammaradiationacomparativestudy AT utpalghosh radiosensitivityandinductionofapoptosisbyhighletcarbonionbeamandlowletgammaradiationacomparativestudy |