Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice
C57BL/6 mice were tested in order to investigate the effects of dietary chitosan (COS) supplements on intestinal microflora and resistance to Citrobacter rodentium infection. The findings reveal that, after consuming a 300 mg/kg COS diet for 14 days, microflora became more diverse as a result of the...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9236196 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551867391934464 |
|---|---|
| author | Guiping Guan Hongbing Wang Shuai Chen Gang Liu Xia Xiong Bie Tan Veeramuthu Duraipandiyan Naif Abdullah Al-Dhabi Jun Fang |
| author_facet | Guiping Guan Hongbing Wang Shuai Chen Gang Liu Xia Xiong Bie Tan Veeramuthu Duraipandiyan Naif Abdullah Al-Dhabi Jun Fang |
| author_sort | Guiping Guan |
| collection | DOAJ |
| description | C57BL/6 mice were tested in order to investigate the effects of dietary chitosan (COS) supplements on intestinal microflora and resistance to Citrobacter rodentium infection. The findings reveal that, after consuming a 300 mg/kg COS diet for 14 days, microflora became more diverse as a result of the supplement. Mice receiving COS exhibited an increase in the percentage of Bacteroidetes phylum and a decrease in the percentage of Firmicutes phylum. After Citrobacter rodentium infection, the histopathology scores indicated that COS feeding resulted in less severe colitis. IL-6 and TNF-α were significantly lower in colon from COS-feeding mice than those in the control group. Furthermore, mice in COS group were also found to experience inhibited activation of nuclear factor-kappa B (NF-κB) in the colonic tissue. Overall, the findings revealed that adding 300 mg/kg COS to the diet changed the composition of the intestinal microflora of mice, resulting in suppressed NF-κB activation and less production of TNF-α and IL-6; and these changes led to better control of inflammation and resolution of infection with C. rodentium. |
| format | Article |
| id | doaj-art-2bad42ee14d14d16b9b31dbd3c364fac |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2bad42ee14d14d16b9b31dbd3c364fac2025-02-03T06:00:18ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/92361969236196Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in MiceGuiping Guan0Hongbing Wang1Shuai Chen2Gang Liu3Xia Xiong4Bie Tan5Veeramuthu Duraipandiyan6Naif Abdullah Al-Dhabi7Jun Fang8College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, ChinaHunan Institute of Animal and Veterinary Science, Changsha, Changsha 410131, ChinaKey Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Co-Innovation Center of Animal Production Safety, Hunan 410125, ChinaKey Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Co-Innovation Center of Animal Production Safety, Hunan 410125, ChinaKey Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Co-Innovation Center of Animal Production Safety, Hunan 410125, ChinaKey Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Co-Innovation Center of Animal Production Safety, Hunan 410125, ChinaDepartment of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box. 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box. 2455, Riyadh 11451, Saudi ArabiaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, ChinaC57BL/6 mice were tested in order to investigate the effects of dietary chitosan (COS) supplements on intestinal microflora and resistance to Citrobacter rodentium infection. The findings reveal that, after consuming a 300 mg/kg COS diet for 14 days, microflora became more diverse as a result of the supplement. Mice receiving COS exhibited an increase in the percentage of Bacteroidetes phylum and a decrease in the percentage of Firmicutes phylum. After Citrobacter rodentium infection, the histopathology scores indicated that COS feeding resulted in less severe colitis. IL-6 and TNF-α were significantly lower in colon from COS-feeding mice than those in the control group. Furthermore, mice in COS group were also found to experience inhibited activation of nuclear factor-kappa B (NF-κB) in the colonic tissue. Overall, the findings revealed that adding 300 mg/kg COS to the diet changed the composition of the intestinal microflora of mice, resulting in suppressed NF-κB activation and less production of TNF-α and IL-6; and these changes led to better control of inflammation and resolution of infection with C. rodentium.http://dx.doi.org/10.1155/2016/9236196 |
| spellingShingle | Guiping Guan Hongbing Wang Shuai Chen Gang Liu Xia Xiong Bie Tan Veeramuthu Duraipandiyan Naif Abdullah Al-Dhabi Jun Fang Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice Mediators of Inflammation |
| title | Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice |
| title_full | Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice |
| title_fullStr | Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice |
| title_full_unstemmed | Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice |
| title_short | Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection in Mice |
| title_sort | dietary chitosan supplementation increases microbial diversity and attenuates the severity of citrobacter rodentium infection in mice |
| url | http://dx.doi.org/10.1155/2016/9236196 |
| work_keys_str_mv | AT guipingguan dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT hongbingwang dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT shuaichen dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT gangliu dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT xiaxiong dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT bietan dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT veeramuthuduraipandiyan dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT naifabdullahaldhabi dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice AT junfang dietarychitosansupplementationincreasesmicrobialdiversityandattenuatestheseverityofcitrobacterrodentiuminfectioninmice |