A Non-Binary Approach to Super-Enhancer Identification and Clustering: A Dataset for Tumor- and Treatment-Associated Dynamics in Mouse Tissues

A Non-Binary Approach to Super-Enhancer Identification and Clustering: A Dataset for Tumor- and Treatment-Associated Dynamics in Mouse Tissues

Super-enhancers (SEs) are large clusters of highly active enhancers that play key regulatory roles in cell identity, development, and disease. While conventional methods classify SEs in a binary fashion—super-enhancer or not—this threshold-based approach can overlook significant intermediate states...

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Bibliographic Details
Main Authors: Ekaterina D. Osintseva, German A. Ashniev, Alexey V. Orlov, Petr I. Nikitin, Zoia G. Zaitseva, Vladimir V. Volkov, Natalia N. Orlova
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Data
Subjects:
super-enhancers
non-binary classification
ChIP-seq
tumor biology
enhancer clustering
epigenetics
Online Access:https://www.mdpi.com/2306-5729/10/5/74
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Summary:Super-enhancers (SEs) are large clusters of highly active enhancers that play key regulatory roles in cell identity, development, and disease. While conventional methods classify SEs in a binary fashion—super-enhancer or not—this threshold-based approach can overlook significant intermediate states of enhancer activity. Here, we present a dataset and accompanying framework that facilitate a more nuanced, non-binary examination of SE activation across mouse tissue types (mammary gland, lung tissue, and NMuMG cells) and various experimental conditions (normal, tumor, and drug-treated samples). By consolidating overlapping SE intervals and capturing continuous enhancer activity metrics (e.g., ChIP-seq signal intensities), our dataset reveals gradual transitions between moderate and high enhancer activity levels that are not captured by strictly binary classification. Additionally, the data include extensive functional annotations, linking SE loci to nearby genes and enabling immediate downstream analyses such as clustering and gene ontology enrichment. The flexible approach supports broader investigations of enhancer landscapes, offering a comprehensive platform for understanding how SE activation underpins disease mechanisms, therapeutic response, and developmental processes.
ISSN:2306-5729

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