Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth
Ferrichrome (Fc) acquisition in Schizosaccharomyces pombe is mediated by the cell-surface siderophore-iron transporter Str1. Here, we report that Str2, a protein homologous to Str1, localizes to the vacuolar membrane. Like Str1, Str2 expression is transcriptionally regulated in response to changes i...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1527727/full |
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| author | Berthy Mbuya Samuel Plante Tobias Vahsen Ariane Brault Simon Labbé |
| author_facet | Berthy Mbuya Samuel Plante Tobias Vahsen Ariane Brault Simon Labbé |
| author_sort | Berthy Mbuya |
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| description | Ferrichrome (Fc) acquisition in Schizosaccharomyces pombe is mediated by the cell-surface siderophore-iron transporter Str1. Here, we report that Str2, a protein homologous to Str1, localizes to the vacuolar membrane. Like Str1, Str2 expression is transcriptionally regulated in response to changes in iron concentrations. Both the str2+ and str1+ genes are induced under low-iron conditions and are repressed by the iron-responsive GATA-type transcription factor Fep1 when iron is abundant. Under high-iron conditions, chromatin immunoprecipitation (ChIP) assays reveal that TAP-Fep1 occupies the str2+ and str1+ promoters. Isolated vacuoles from str2Δ fep1Δ cells expressing GFP-tagged Str2 exhibit iron accumulation in vacuoles upon exposure to exogenous holo-Fc. sib1Δ sib2Δ cells deficient in Fc biosynthesis and lacking the str2+ gene (str2Δ) are unable to grow in the presence of exogenous Fc as a sole source of iron. Further analysis identified that conserved amino acids Tyr539 and Tyr553 in the last predicted loop of Str2 are required for supporting Fc-dependent growth of a sib1Δ sib2Δ mutant strain. Collectively, these findings indicate that the vacuolar Str2 protein plays a role in the consumption of Fc as an iron source, while also revealing the involvement of the vacuole in iron release from exogenous Fc after its assimilation. |
| format | Article |
| id | doaj-art-2b38a99c86594eb2b45b7143e1d72caa |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-2b38a99c86594eb2b45b7143e1d72caa2025-02-06T12:12:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15277271527727Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growthBerthy MbuyaSamuel PlanteTobias VahsenAriane BraultSimon LabbéFerrichrome (Fc) acquisition in Schizosaccharomyces pombe is mediated by the cell-surface siderophore-iron transporter Str1. Here, we report that Str2, a protein homologous to Str1, localizes to the vacuolar membrane. Like Str1, Str2 expression is transcriptionally regulated in response to changes in iron concentrations. Both the str2+ and str1+ genes are induced under low-iron conditions and are repressed by the iron-responsive GATA-type transcription factor Fep1 when iron is abundant. Under high-iron conditions, chromatin immunoprecipitation (ChIP) assays reveal that TAP-Fep1 occupies the str2+ and str1+ promoters. Isolated vacuoles from str2Δ fep1Δ cells expressing GFP-tagged Str2 exhibit iron accumulation in vacuoles upon exposure to exogenous holo-Fc. sib1Δ sib2Δ cells deficient in Fc biosynthesis and lacking the str2+ gene (str2Δ) are unable to grow in the presence of exogenous Fc as a sole source of iron. Further analysis identified that conserved amino acids Tyr539 and Tyr553 in the last predicted loop of Str2 are required for supporting Fc-dependent growth of a sib1Δ sib2Δ mutant strain. Collectively, these findings indicate that the vacuolar Str2 protein plays a role in the consumption of Fc as an iron source, while also revealing the involvement of the vacuole in iron release from exogenous Fc after its assimilation.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1527727/fullfission yeastferrichromeironiron-regulatory GATA-type transcription factorsiderophore transporter |
| spellingShingle | Berthy Mbuya Samuel Plante Tobias Vahsen Ariane Brault Simon Labbé Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth Frontiers in Microbiology fission yeast ferrichrome iron iron-regulatory GATA-type transcription factor siderophore transporter |
| title | Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth |
| title_full | Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth |
| title_fullStr | Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth |
| title_full_unstemmed | Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth |
| title_short | Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth |
| title_sort | fission yeast cells deficient in siderophore biosynthesis require str2 for ferrichrome dependent growth |
| topic | fission yeast ferrichrome iron iron-regulatory GATA-type transcription factor siderophore transporter |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1527727/full |
| work_keys_str_mv | AT berthymbuya fissionyeastcellsdeficientinsiderophorebiosynthesisrequirestr2forferrichromedependentgrowth AT samuelplante fissionyeastcellsdeficientinsiderophorebiosynthesisrequirestr2forferrichromedependentgrowth AT tobiasvahsen fissionyeastcellsdeficientinsiderophorebiosynthesisrequirestr2forferrichromedependentgrowth AT arianebrault fissionyeastcellsdeficientinsiderophorebiosynthesisrequirestr2forferrichromedependentgrowth AT simonlabbe fissionyeastcellsdeficientinsiderophorebiosynthesisrequirestr2forferrichromedependentgrowth |