Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding
ABSTRACT Marine metazoan biodiversity is accretively being explored through environmental DNA (eDNA) metabarcoding of seawater. However, knowledge gaps in the use of eDNA to study changes in diversity resulting from changing abiotic conditions still do exist. In order to address these gaps, we analy...
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| Format: | Article |
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Wiley
2024-11-01
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| Series: | Environmental DNA |
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| Online Access: | https://doi.org/10.1002/edn3.70037 |
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| author | Alica Ohnesorge Uwe John Lucie Kuczynski Stefan Neuhaus Kingsly Chuo Beng Bernd Krock Silke Laakmann |
| author_facet | Alica Ohnesorge Uwe John Lucie Kuczynski Stefan Neuhaus Kingsly Chuo Beng Bernd Krock Silke Laakmann |
| author_sort | Alica Ohnesorge |
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| description | ABSTRACT Marine metazoan biodiversity is accretively being explored through environmental DNA (eDNA) metabarcoding of seawater. However, knowledge gaps in the use of eDNA to study changes in diversity resulting from changing abiotic conditions still do exist. In order to address these gaps, we analyzed patterns of marine invertebrate biodiversity based on eDNA from water and sediment samples along a decreasing salinity gradient from the North Sea toward the Baltic Sea. eDNA was collected from surface (SW) and bottom (BW) water, and from the uppermost sediment layer (SE). To supplement the eDNA approach, we conducted parallel zooplankton (ZP) metabarcoding and morphological identification. DNA was extracted from eDNA and ZP samples, amplified using two universal primers that target of the mitochondrial cytochrome c oxidase subunit 1 (COI) and the nuclear ribosomal 18S rRNA genes, and paired‐end sequenced on Illumina Miseq. Metabarcoding detected 279 metazoan species (from 16 phyla) of which > 87% are known from the study area or adjacent regions. Communities identified in SW eDNA were a subset of communities identified in ZP metabarcoding. BW eDNA had additional benthic (mainly bivalve) species. Communities identified in SE eDNA were distinct from those in water eDNA and ZP metabarcoding, and mainly represented by in‐ and meiofauna. Out of all approaches, only ZP metabarcoding uncovered the expected decrease in species richness toward brackish conditions. Neither salinity nor spatial distance had a significant effect on species composition. All approaches revealed regional differences of which SE eDNA was least informative. The detection of holoplanktonic species from SE eDNA provided evidence for sinking of eDNA particles, dead organisms or the presence of resting eggs. Our study confirms the value of metabarcoding to identify the North Sea and Baltic Sea invertebrates and underscores the importance of combining multiple approaches to understand invertebrate biodiversity and its change in the marine realm. |
| format | Article |
| id | doaj-art-2b32b77c3dcc4e5cb4944ae30846c6a7 |
| institution | Kabale University |
| issn | 2637-4943 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
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| series | Environmental DNA |
| spelling | doaj-art-2b32b77c3dcc4e5cb4944ae30846c6a72025-01-29T05:11:50ZengWileyEnvironmental DNA2637-49432024-11-0166n/an/a10.1002/edn3.70037Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA MetabarcodingAlica Ohnesorge0Uwe John1Lucie Kuczynski2Stefan Neuhaus3Kingsly Chuo Beng4Bernd Krock5Silke Laakmann6Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB) Oldenburg GermanyHelmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB) Oldenburg GermanyInstitute for Chemistry and Biology of Marine Environments (ICBM), Carl von Ossietzky Universität Oldenburg Wilhelmshaven GermanyAlfred‐Wegener‐Institut Helmholtz‐Zentrum für Polar‐ und Meeresforschung (AWI) Bremerhaven GermanyHelmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB) Oldenburg GermanyAlfred‐Wegener‐Institut Helmholtz‐Zentrum für Polar‐ und Meeresforschung (AWI) Bremerhaven GermanyHelmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB) Oldenburg GermanyABSTRACT Marine metazoan biodiversity is accretively being explored through environmental DNA (eDNA) metabarcoding of seawater. However, knowledge gaps in the use of eDNA to study changes in diversity resulting from changing abiotic conditions still do exist. In order to address these gaps, we analyzed patterns of marine invertebrate biodiversity based on eDNA from water and sediment samples along a decreasing salinity gradient from the North Sea toward the Baltic Sea. eDNA was collected from surface (SW) and bottom (BW) water, and from the uppermost sediment layer (SE). To supplement the eDNA approach, we conducted parallel zooplankton (ZP) metabarcoding and morphological identification. DNA was extracted from eDNA and ZP samples, amplified using two universal primers that target of the mitochondrial cytochrome c oxidase subunit 1 (COI) and the nuclear ribosomal 18S rRNA genes, and paired‐end sequenced on Illumina Miseq. Metabarcoding detected 279 metazoan species (from 16 phyla) of which > 87% are known from the study area or adjacent regions. Communities identified in SW eDNA were a subset of communities identified in ZP metabarcoding. BW eDNA had additional benthic (mainly bivalve) species. Communities identified in SE eDNA were distinct from those in water eDNA and ZP metabarcoding, and mainly represented by in‐ and meiofauna. Out of all approaches, only ZP metabarcoding uncovered the expected decrease in species richness toward brackish conditions. Neither salinity nor spatial distance had a significant effect on species composition. All approaches revealed regional differences of which SE eDNA was least informative. The detection of holoplanktonic species from SE eDNA provided evidence for sinking of eDNA particles, dead organisms or the presence of resting eggs. Our study confirms the value of metabarcoding to identify the North Sea and Baltic Sea invertebrates and underscores the importance of combining multiple approaches to understand invertebrate biodiversity and its change in the marine realm.https://doi.org/10.1002/edn3.70037biodiversitymarine faunaspatial resolutionzooplankton |
| spellingShingle | Alica Ohnesorge Uwe John Lucie Kuczynski Stefan Neuhaus Kingsly Chuo Beng Bernd Krock Silke Laakmann Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding Environmental DNA biodiversity marine fauna spatial resolution zooplankton |
| title | Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding |
| title_full | Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding |
| title_fullStr | Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding |
| title_full_unstemmed | Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding |
| title_short | Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding |
| title_sort | roaming the seas assessing marine invertebrate biodiversity along salinity gradients with zooplankton and edna metabarcoding |
| topic | biodiversity marine fauna spatial resolution zooplankton |
| url | https://doi.org/10.1002/edn3.70037 |
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