Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy?
Membranous nephropathy (MN) is a kidney specific autoimmune disease mainly mediated by anti-phospholipase A2 receptor 1 autoantibody (PLA2R1 Ab). The adequate assessment of chimeric anti-CD20 monoclonal antibody, rituximab (RTX), efficacy is still needed to improve clinical outcome of patient with M...
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/7651024 |
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| author | Agnieszka Pozdzik Ingrid Beukinga Chunyan Gu-Trantien Karen Willard-Gallo Joëlle Nortier Olivier Pradier |
| author_facet | Agnieszka Pozdzik Ingrid Beukinga Chunyan Gu-Trantien Karen Willard-Gallo Joëlle Nortier Olivier Pradier |
| author_sort | Agnieszka Pozdzik |
| collection | DOAJ |
| description | Membranous nephropathy (MN) is a kidney specific autoimmune disease mainly mediated by anti-phospholipase A2 receptor 1 autoantibody (PLA2R1 Ab). The adequate assessment of chimeric anti-CD20 monoclonal antibody, rituximab (RTX), efficacy is still needed to improve clinical outcome of patient with MN. We evaluated the modification of plasmablasts (CD3−CD19+CD20−IgD−CD27highCD38high), a useful biomarker of RTX response in other autoimmune diseases, and memory (CD3−CD19+CD20+IgD−CD27+CD38−) and naive (CD3−CD19+CD20+IgD+CD27−CD38low) B cells by fluorescence-activated cell sorter analysis in PLA2R1 related MN in one patient during the 4 years of follow-up after RTX. RTX induced complete disappearance of CD19+ B cells, plasmablasts, and memory B cells as soon as day 15. Despite severe CD19+ lymphopenia, plasmablasts and memory B cells reemerged early before naive B cells (days 45, 90, and 120, resp.). During the follow-up, plasmablasts decreased more rapidly than memory B cells but still remained elevated as compared to day 0 of RTX. Concomitantly, anti-PLA2R1 Ab increased progressively. Our single case report suggests that, besides monitoring of serum anti-PLA2R1 Ab level, enumeration of circulating plasmablasts and memory B cells represents an attractive and complementary tool to assess immunological activity and efficacy of RTX induced B cells depletion in anti-PLA2R1 Ab related MN. |
| format | Article |
| id | doaj-art-2b22481ad4e044bca08900c5c82b80ff |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2b22481ad4e044bca08900c5c82b80ff2025-02-03T01:09:10ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/76510247651024Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy?Agnieszka Pozdzik0Ingrid Beukinga1Chunyan Gu-Trantien2Karen Willard-Gallo3Joëlle Nortier4Olivier Pradier5Department of Nephrology, Dialysis and Renal Transplantation, Cliniques Universitaires de Bruxelles (CUB), Erasme Hospital, 1070 Brussels, BelgiumDepartment of Hematology, Cliniques Universitaires de Bruxelles (CUB), Erasme Hospital, 1070 Brussels, BelgiumUnit of Molecular Immunology, Institute Jules Bordet, Université Libre de Bruxelles (ULB), 1000 Brussels, BelgiumUnit of Molecular Immunology, Institute Jules Bordet, Université Libre de Bruxelles (ULB), 1000 Brussels, BelgiumDepartment of Nephrology, Dialysis and Renal Transplantation, Cliniques Universitaires de Bruxelles (CUB), Erasme Hospital, 1070 Brussels, BelgiumDepartment of Hematology, Cliniques Universitaires de Bruxelles (CUB), Erasme Hospital, 1070 Brussels, BelgiumMembranous nephropathy (MN) is a kidney specific autoimmune disease mainly mediated by anti-phospholipase A2 receptor 1 autoantibody (PLA2R1 Ab). The adequate assessment of chimeric anti-CD20 monoclonal antibody, rituximab (RTX), efficacy is still needed to improve clinical outcome of patient with MN. We evaluated the modification of plasmablasts (CD3−CD19+CD20−IgD−CD27highCD38high), a useful biomarker of RTX response in other autoimmune diseases, and memory (CD3−CD19+CD20+IgD−CD27+CD38−) and naive (CD3−CD19+CD20+IgD+CD27−CD38low) B cells by fluorescence-activated cell sorter analysis in PLA2R1 related MN in one patient during the 4 years of follow-up after RTX. RTX induced complete disappearance of CD19+ B cells, plasmablasts, and memory B cells as soon as day 15. Despite severe CD19+ lymphopenia, plasmablasts and memory B cells reemerged early before naive B cells (days 45, 90, and 120, resp.). During the follow-up, plasmablasts decreased more rapidly than memory B cells but still remained elevated as compared to day 0 of RTX. Concomitantly, anti-PLA2R1 Ab increased progressively. Our single case report suggests that, besides monitoring of serum anti-PLA2R1 Ab level, enumeration of circulating plasmablasts and memory B cells represents an attractive and complementary tool to assess immunological activity and efficacy of RTX induced B cells depletion in anti-PLA2R1 Ab related MN.http://dx.doi.org/10.1155/2016/7651024 |
| spellingShingle | Agnieszka Pozdzik Ingrid Beukinga Chunyan Gu-Trantien Karen Willard-Gallo Joëlle Nortier Olivier Pradier Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? Mediators of Inflammation |
| title | Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? |
| title_full | Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? |
| title_fullStr | Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? |
| title_full_unstemmed | Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? |
| title_short | Circulating (CD3−CD19+CD20−IgD−CD27highCD38high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy? |
| title_sort | circulating cd3 cd19 cd20 igd cd27highcd38high plasmablasts a promising cellular biomarker for immune activity for anti pla2r1 related membranous nephropathy |
| url | http://dx.doi.org/10.1155/2016/7651024 |
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