Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition
Laboratory non-human primates (NHPs) are commonly subjected to social deprivation in various scientific researches. However, the impact of social deprivation on gut microbiome remains largely unknown. We examined the health status and gut microbiota of female cynomolgus monkeys housed in isolation o...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025002920 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573064089436160 |
|---|---|
| author | Yunpeng Yang Yong Lu Changshan Gao Yanhong Nie Hongfei Wang Yufei Huang Haiyan Dong Qiang Sun |
| author_facet | Yunpeng Yang Yong Lu Changshan Gao Yanhong Nie Hongfei Wang Yufei Huang Haiyan Dong Qiang Sun |
| author_sort | Yunpeng Yang |
| collection | DOAJ |
| description | Laboratory non-human primates (NHPs) are commonly subjected to social deprivation in various scientific researches. However, the impact of social deprivation on gut microbiome remains largely unknown. We examined the health status and gut microbiota of female cynomolgus monkeys housed in isolation or social conditions and found that social deprivation brought adverse effects to monkeys by inhibiting their growth, remodeling the immune status, and decreasing the level of beneficial biochemical parameters. 16S rRNA gene sequencing revealed that the gut microbial composition and function differed between grouped and isolated monkeys. Specifically, grouping the single-caged young monkeys to socially housed condition could decrease the relative abundance of Firmicutes and increase the relative abundance of Bacteroidetes, while separating the socially housed middle-aged monkeys into single cages showed the opposite trend. Besides, training female monkeys to detect menstruation under socially-housed condition could increase their body weight change and adjusting their immune status, thus attenuating the adverse effects of separating them to single cages. Our results verified the significant role of grouping in mitigating adverse health and microbiota alterations caused by isolation in female cynomolgus monkeys and emphasized the importance of training NHPs to cooperate with experimental procedures under socially housed condition, which could not only improve the welfare of cynomolgus monkeys but also enhance the accuracy and reliability of scientific results. |
| format | Article |
| id | doaj-art-2aeab31d355b4915a95c15cbe52b0824 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-2aeab31d355b4915a95c15cbe52b08242025-02-02T05:28:32ZengElsevierHeliyon2405-84402025-01-01112e41912Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed conditionYunpeng Yang0Yong Lu1Changshan Gao2Yanhong Nie3Hongfei Wang4Yufei Huang5Haiyan Dong6Qiang Sun7Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China; Institute of Comparative Medicine, Yangzhou University, Yangzhou, 225009, China; Institute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China; Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 201600, China; Corresponding author. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.Institute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, ChinaInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, ChinaInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China; Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 201600, ChinaInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China; Institute of Comparative Medicine, Yangzhou University, Yangzhou, 225009, ChinaInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, ChinaInstitute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China; Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 201600, China; Corresponding author. Institute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.Laboratory non-human primates (NHPs) are commonly subjected to social deprivation in various scientific researches. However, the impact of social deprivation on gut microbiome remains largely unknown. We examined the health status and gut microbiota of female cynomolgus monkeys housed in isolation or social conditions and found that social deprivation brought adverse effects to monkeys by inhibiting their growth, remodeling the immune status, and decreasing the level of beneficial biochemical parameters. 16S rRNA gene sequencing revealed that the gut microbial composition and function differed between grouped and isolated monkeys. Specifically, grouping the single-caged young monkeys to socially housed condition could decrease the relative abundance of Firmicutes and increase the relative abundance of Bacteroidetes, while separating the socially housed middle-aged monkeys into single cages showed the opposite trend. Besides, training female monkeys to detect menstruation under socially-housed condition could increase their body weight change and adjusting their immune status, thus attenuating the adverse effects of separating them to single cages. Our results verified the significant role of grouping in mitigating adverse health and microbiota alterations caused by isolation in female cynomolgus monkeys and emphasized the importance of training NHPs to cooperate with experimental procedures under socially housed condition, which could not only improve the welfare of cynomolgus monkeys but also enhance the accuracy and reliability of scientific results.http://www.sciencedirect.com/science/article/pii/S2405844025002920 |
| spellingShingle | Yunpeng Yang Yong Lu Changshan Gao Yanhong Nie Hongfei Wang Yufei Huang Haiyan Dong Qiang Sun Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition Heliyon |
| title | Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition |
| title_full | Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition |
| title_fullStr | Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition |
| title_full_unstemmed | Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition |
| title_short | Effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition |
| title_sort | effects of housing conditions on health and gut microbiome of female cynomolgus monkeys and improvement of welfare by checking menstruation under socially housed condition |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025002920 |
| work_keys_str_mv | AT yunpengyang effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT yonglu effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT changshangao effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT yanhongnie effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT hongfeiwang effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT yufeihuang effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT haiyandong effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition AT qiangsun effectsofhousingconditionsonhealthandgutmicrobiomeoffemalecynomolgusmonkeysandimprovementofwelfarebycheckingmenstruationundersociallyhousedcondition |