Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars
Garlic (Allium sativum L.) has a reputation as a therapeutic agent for many different diseases such as microbial infections, hypertension, hypercholesterolaemia, diabetes, atherosclerosis and cancer. Health benefits of garlic depend on its content of biologically-active compounds, which differs betw...
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| Format: | Article |
| Language: | English |
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2018-03-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.degruyter.com/view/j/pjfns.2018.68.issue-1/pjfns-2017-0005/pjfns-2017-0005.xml?format=INT |
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| author | Szychowski Konrad A. Rybczyńska-Tkaczyk Kamila Gaweł-Bęben Katarzyna Świeca Michał Karaś Monika Jakubczyk Anna Matysiak Magdalena Binduga Urszula E. Gmiński Jan |
| author_facet | Szychowski Konrad A. Rybczyńska-Tkaczyk Kamila Gaweł-Bęben Katarzyna Świeca Michał Karaś Monika Jakubczyk Anna Matysiak Magdalena Binduga Urszula E. Gmiński Jan |
| author_sort | Szychowski Konrad A. |
| collection | DOAJ |
| description | Garlic (Allium sativum L.) has a reputation as a therapeutic agent for many different diseases such as microbial infections, hypertension, hypercholesterolaemia, diabetes, atherosclerosis and cancer. Health benefits of garlic depend on its content of biologically-active compounds, which differs between cultivars and geographical regions. The aim of this study was to evaluate and compare the biological activity of aqueous extracts from nine garlic varieties from different countries (Poland, Spain, China, Portugal, Burma, Thailand and Uzbekistan). Antioxidant properties were evaluated through free radical scavenging (DPPH•, ABTS•+) and ion chelation (Fe2+, Cu2+) activities. The cytotoxicity of garlic extracts was evaluated in vitro using Neutral Red Uptake assay in normal human skin fibroblasts. The obtained results revealed that garlic extracts contained the highest amount of syringic and p-hydroxybenzoic acids derivatives. The lowest IC50 values for DPPH•, ABTS•+ scavenging and Cu2+ chelating ability were determined in Chinese garlic extracts (4.63, 0.43 and 14.90 μg/mL, respectively). Extracts from Spanish cultivar Morado and Chinese garlic were highly cytotoxic to human skin fibroblasts as they reduced cellular proliferation by 70–90%. We showed diverse contents of proteins and phenolic components in garlic bulbs from different varieties. The obtained results could help to choose the cultivars of garlic which contain significant amounts of active compounds, have important antioxidant properties and display low antiproliferative effect and/or low cytotoxicity against normal human skin fibroblast BJ. |
| format | Article |
| id | doaj-art-2ad8c3820d9d44fbbde6a6c348b438c6 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-2ad8c3820d9d44fbbde6a6c348b438c62025-02-02T03:28:58ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072018-03-01681738110.1515/pjfns-2017-0005pjfns-2017-0005Characterization of Active Compounds of Different Garlic (Allium sativum L.) CultivarsSzychowski Konrad A.0Rybczyńska-Tkaczyk Kamila1Gaweł-Bęben Katarzyna2Świeca Michał3Karaś Monika4Jakubczyk Anna5Matysiak Magdalena6Binduga Urszula E.7Gmiński Jan8Department of Public Health, Dietetics and Lifestyle Disorders, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35–225Rzeszow, PolandDepartment of Environmental Microbiology, Laboratory of Mycology, University of Life Sciences, Leszczyńskiego 7, 20–069 Lublin, PolandDepartment of Cosmetology, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35–225Rzeszow, PolandDepartment of Biochemistry and Food Chemistry, University of Life Sciences, Skromna 8, 20–704Lublin, PolandDepartment of Biochemistry and Food Chemistry, University of Life Sciences, Skromna 8, 20–704Lublin, PolandDepartment of Biochemistry and Food Chemistry, University of Life Sciences, Skromna 8, 20–704Lublin, PolandDepartment of Molecular Biology and Translational Research, Institute of Rural Health, Lublin, Poland, Jaczewskiego 2, 20–090Lublin, PolandDepartment of Public Health, Dietetics and Lifestyle Disorders, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35–225Rzeszow, PolandDepartment of Public Health, Dietetics and Lifestyle Disorders, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35–225Rzeszow, PolandGarlic (Allium sativum L.) has a reputation as a therapeutic agent for many different diseases such as microbial infections, hypertension, hypercholesterolaemia, diabetes, atherosclerosis and cancer. Health benefits of garlic depend on its content of biologically-active compounds, which differs between cultivars and geographical regions. The aim of this study was to evaluate and compare the biological activity of aqueous extracts from nine garlic varieties from different countries (Poland, Spain, China, Portugal, Burma, Thailand and Uzbekistan). Antioxidant properties were evaluated through free radical scavenging (DPPH•, ABTS•+) and ion chelation (Fe2+, Cu2+) activities. The cytotoxicity of garlic extracts was evaluated in vitro using Neutral Red Uptake assay in normal human skin fibroblasts. The obtained results revealed that garlic extracts contained the highest amount of syringic and p-hydroxybenzoic acids derivatives. The lowest IC50 values for DPPH•, ABTS•+ scavenging and Cu2+ chelating ability were determined in Chinese garlic extracts (4.63, 0.43 and 14.90 μg/mL, respectively). Extracts from Spanish cultivar Morado and Chinese garlic were highly cytotoxic to human skin fibroblasts as they reduced cellular proliferation by 70–90%. We showed diverse contents of proteins and phenolic components in garlic bulbs from different varieties. The obtained results could help to choose the cultivars of garlic which contain significant amounts of active compounds, have important antioxidant properties and display low antiproliferative effect and/or low cytotoxicity against normal human skin fibroblast BJ.http://www.degruyter.com/view/j/pjfns.2018.68.issue-1/pjfns-2017-0005/pjfns-2017-0005.xml?format=INTgarlicAllium sativumantioxidantchromatographyion chelationcytotoxicity |
| spellingShingle | Szychowski Konrad A. Rybczyńska-Tkaczyk Kamila Gaweł-Bęben Katarzyna Świeca Michał Karaś Monika Jakubczyk Anna Matysiak Magdalena Binduga Urszula E. Gmiński Jan Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars Polish Journal of Food and Nutrition Sciences garlic Allium sativum antioxidant chromatography ion chelation cytotoxicity |
| title | Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars |
| title_full | Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars |
| title_fullStr | Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars |
| title_full_unstemmed | Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars |
| title_short | Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars |
| title_sort | characterization of active compounds of different garlic allium sativum l cultivars |
| topic | garlic Allium sativum antioxidant chromatography ion chelation cytotoxicity |
| url | http://www.degruyter.com/view/j/pjfns.2018.68.issue-1/pjfns-2017-0005/pjfns-2017-0005.xml?format=INT |
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