Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut
Understanding the honeybee gut bacteria is an essential aspect as honeybees are the primary pollinators of many crops. In this study, the honeybee-associated gut bacteria were investigated by targeting the V3-V4 region of 16S rRNA genes using the Illumina MiSeq. The adult worker was captured in an u...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/6906921 |
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| _version_ | 1832560168333737984 |
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| author | Christian Apolinaris Lombogia Max Tulung Jimmy Posangi Trina Ekawati Tallei |
| author_facet | Christian Apolinaris Lombogia Max Tulung Jimmy Posangi Trina Ekawati Tallei |
| author_sort | Christian Apolinaris Lombogia |
| collection | DOAJ |
| description | Understanding the honeybee gut bacteria is an essential aspect as honeybees are the primary pollinators of many crops. In this study, the honeybee-associated gut bacteria were investigated by targeting the V3-V4 region of 16S rRNA genes using the Illumina MiSeq. The adult worker was captured in an urban area in a dense settlement. In total, 83,018 reads were obtained, revealing six phyla from 749 bacterial operational taxonomic units (OTUs). The gut was dominated by Proteobacteria (58% of the total reads, including Enterobacteriaceae 28.2%, Erwinia 6.43%, and Klebsiella 4.90%), Firmicutes (29% of the total reads, including Lactococcus garvieae 13.45%, Lactobacillus spp. 8.19%, and Enterococcus spp. 4.47%), and Actinobacteria (8% of the total reads, including Bifidobacterium spp. 7.96%). Many of these bacteria belong to the group of lactic acid bacteria (LAB), which was claimed to be composed of beneficial bacteria involved in maintaining a healthy host. The honeybee was identified as Apis nigrocincta based on an identity BLAST search of its COI region. This study is the first report on the gut microbial community structure and composition of A. nigrocincta from Indonesia. |
| format | Article |
| id | doaj-art-2acfa538d8e8471fa07bd8a86583f509 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-2acfa538d8e8471fa07bd8a86583f5092025-02-03T01:28:17ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/69069216906921Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta GutChristian Apolinaris Lombogia0Max Tulung1Jimmy Posangi2Trina Ekawati Tallei3Entomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, North Sulawesi, IndonesiaEntomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, North Sulawesi, IndonesiaEntomology Study Program, Postgraduate Program, Sam Ratulangi University, Manado, North Sulawesi, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, North Sulawesi, IndonesiaUnderstanding the honeybee gut bacteria is an essential aspect as honeybees are the primary pollinators of many crops. In this study, the honeybee-associated gut bacteria were investigated by targeting the V3-V4 region of 16S rRNA genes using the Illumina MiSeq. The adult worker was captured in an urban area in a dense settlement. In total, 83,018 reads were obtained, revealing six phyla from 749 bacterial operational taxonomic units (OTUs). The gut was dominated by Proteobacteria (58% of the total reads, including Enterobacteriaceae 28.2%, Erwinia 6.43%, and Klebsiella 4.90%), Firmicutes (29% of the total reads, including Lactococcus garvieae 13.45%, Lactobacillus spp. 8.19%, and Enterococcus spp. 4.47%), and Actinobacteria (8% of the total reads, including Bifidobacterium spp. 7.96%). Many of these bacteria belong to the group of lactic acid bacteria (LAB), which was claimed to be composed of beneficial bacteria involved in maintaining a healthy host. The honeybee was identified as Apis nigrocincta based on an identity BLAST search of its COI region. This study is the first report on the gut microbial community structure and composition of A. nigrocincta from Indonesia.http://dx.doi.org/10.1155/2020/6906921 |
| spellingShingle | Christian Apolinaris Lombogia Max Tulung Jimmy Posangi Trina Ekawati Tallei Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut International Journal of Microbiology |
| title | Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut |
| title_full | Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut |
| title_fullStr | Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut |
| title_full_unstemmed | Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut |
| title_short | Bacterial Composition, Community Structure, and Diversity in Apis nigrocincta Gut |
| title_sort | bacterial composition community structure and diversity in apis nigrocincta gut |
| url | http://dx.doi.org/10.1155/2020/6906921 |
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