Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners
Two simple spectrophotometric methods have been proposed for simultaneous determination of two colorants (Indigotin and Brilliant Blue) and two sweeteners (Acesulfame-K and Aspartame) in synthetic mixtures and chewing gums without any prior separation or purification. The first method, derivative sp...
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/127847 |
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| author | Fatma Turak Mahmure Ustun Ozgur |
| author_facet | Fatma Turak Mahmure Ustun Ozgur |
| author_sort | Fatma Turak |
| collection | DOAJ |
| description | Two simple spectrophotometric methods have been proposed for simultaneous determination of two colorants (Indigotin and Brilliant Blue) and two sweeteners (Acesulfame-K and Aspartame) in synthetic mixtures and chewing gums without any prior separation or purification. The first method, derivative spectrophotometry (ZCDS), is based on recording the first derivative curves (for Indigotin, Brillant Blue, and Acesulfame-K) and third-derivative curve (for Aspartame) and determining each component using the zero-crossing technique. The other method, ratio derivative spectrophotometry (RDS), depends on application ratio spectra of first- and third-derivative spectrophotometry to resolve the interference due to spectral overlapping. Both colorants and sweeteners showed good linearity, with regression coefficients of 0.9992–0.9999. The LOD and LOQ values ranged from 0.05 to 0.33 μgmL−1 and from 0.06 to 0.47 μgmL−1, respectively. The intraday and interday precision tests produced good RSD% values (<0.81%); recoveries ranged from 99.78% to 100.67% for all two methods. The accuracy and precision of the methods have been determined, and the methods have been validated by analyzing synthetic mixtures containing colorants and sweeteners. Two methods were applied for the above combination, and satisfactory results were obtained. The results obtained by applying the ZCDS method were statistically compared with those obtained by the RDS method. |
| format | Article |
| id | doaj-art-2a971402b2e444808f1747f46986e3a2 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | Journal of Chemistry |
| spelling | doaj-art-2a971402b2e444808f1747f46986e3a22025-02-03T01:02:31ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/127847127847Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and SweetenersFatma Turak0Mahmure Ustun Ozgur1Department of Chemistry, Faculty of Science and Art, Yıldız Technical University, 34220 Istanbul, TurkeyDepartment of Chemistry, Faculty of Science and Art, Yıldız Technical University, 34220 Istanbul, TurkeyTwo simple spectrophotometric methods have been proposed for simultaneous determination of two colorants (Indigotin and Brilliant Blue) and two sweeteners (Acesulfame-K and Aspartame) in synthetic mixtures and chewing gums without any prior separation or purification. The first method, derivative spectrophotometry (ZCDS), is based on recording the first derivative curves (for Indigotin, Brillant Blue, and Acesulfame-K) and third-derivative curve (for Aspartame) and determining each component using the zero-crossing technique. The other method, ratio derivative spectrophotometry (RDS), depends on application ratio spectra of first- and third-derivative spectrophotometry to resolve the interference due to spectral overlapping. Both colorants and sweeteners showed good linearity, with regression coefficients of 0.9992–0.9999. The LOD and LOQ values ranged from 0.05 to 0.33 μgmL−1 and from 0.06 to 0.47 μgmL−1, respectively. The intraday and interday precision tests produced good RSD% values (<0.81%); recoveries ranged from 99.78% to 100.67% for all two methods. The accuracy and precision of the methods have been determined, and the methods have been validated by analyzing synthetic mixtures containing colorants and sweeteners. Two methods were applied for the above combination, and satisfactory results were obtained. The results obtained by applying the ZCDS method were statistically compared with those obtained by the RDS method.http://dx.doi.org/10.1155/2013/127847 |
| spellingShingle | Fatma Turak Mahmure Ustun Ozgur Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners Journal of Chemistry |
| title | Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners |
| title_full | Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners |
| title_fullStr | Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners |
| title_full_unstemmed | Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners |
| title_short | Validated Spectrophotometric Methods for Simultaneous Determination of Food Colorants and Sweeteners |
| title_sort | validated spectrophotometric methods for simultaneous determination of food colorants and sweeteners |
| url | http://dx.doi.org/10.1155/2013/127847 |
| work_keys_str_mv | AT fatmaturak validatedspectrophotometricmethodsforsimultaneousdeterminationoffoodcolorantsandsweeteners AT mahmureustunozgur validatedspectrophotometricmethodsforsimultaneousdeterminationoffoodcolorantsandsweeteners |