Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients

Although various plant protein (PP) ingredients are available on the market, their application in foods is not trivial, and food companies are struggling to identify PP ingredients fitting the intended use. To fill this gap, abundant literature has appeared but data are hardly comparable due to the...

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Main Authors: Lorenzo Barozzi, Stella Plazzotta, Ada Nucci, Lara Manzocco
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665927125000024
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author Lorenzo Barozzi
Stella Plazzotta
Ada Nucci
Lara Manzocco
author_facet Lorenzo Barozzi
Stella Plazzotta
Ada Nucci
Lara Manzocco
author_sort Lorenzo Barozzi
collection DOAJ
description Although various plant protein (PP) ingredients are available on the market, their application in foods is not trivial, and food companies are struggling to identify PP ingredients fitting the intended use. To fill this gap, abundant literature has appeared but data are hardly comparable due to the absence of a recognized classification of PP ingredients accounting not only for protein purity but also for the process history, and of standardised protocols for technological functionality assessment. In this review, a comprehensive analysis of comparable literature data was thus carried out to elucidate the effect of composition and processing variables on PP technological functionalities. The review presents four sections describing: (i) the approach followed for the construction of a database of PP ingredient functionalities; (ii) the composition and processing factors relevant to PP ingredients; (iii) PP ingredient functional properties and methods used for their determination; (iv) the effect of composition and processing factors on PP ingredient functionalities. This analysis showed legume proteins to present the highest solubility and interfacial properties while pseudocereal ones the highest water-holding capacity. Although pure ingredients show higher functionalities, non-protein components could contribute to interfacial properties. Alkaline extraction, isoelectric precipitation and freeze-drying is the process mostly used in academic research to obtain PP ingredients. However, other extraction, purification, and drying methods can be properly combined, resulting in specific PP ingredient functionalities. Overall, this review highlights that, besides protein purity and source, knowledge of the processing history is required to select PP ingredients with desired functionalities.
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spelling doaj-art-2a7d286beee94fe69b4cbf89f5eff9c32025-01-24T04:45:41ZengElsevierCurrent Research in Food Science2665-92712025-01-0110100971Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredientsLorenzo Barozzi0Stella Plazzotta1Ada Nucci2Lara Manzocco3Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via Sondrio 2/a, 33100, Udine, ItalyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via Sondrio 2/a, 33100, Udine, Italy; Corresponding author.Lavazza innovation Center, Luigi Lavazza s.p.a., Str. di Settimo, 10156, Famolenta, ItalyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via Sondrio 2/a, 33100, Udine, ItalyAlthough various plant protein (PP) ingredients are available on the market, their application in foods is not trivial, and food companies are struggling to identify PP ingredients fitting the intended use. To fill this gap, abundant literature has appeared but data are hardly comparable due to the absence of a recognized classification of PP ingredients accounting not only for protein purity but also for the process history, and of standardised protocols for technological functionality assessment. In this review, a comprehensive analysis of comparable literature data was thus carried out to elucidate the effect of composition and processing variables on PP technological functionalities. The review presents four sections describing: (i) the approach followed for the construction of a database of PP ingredient functionalities; (ii) the composition and processing factors relevant to PP ingredients; (iii) PP ingredient functional properties and methods used for their determination; (iv) the effect of composition and processing factors on PP ingredient functionalities. This analysis showed legume proteins to present the highest solubility and interfacial properties while pseudocereal ones the highest water-holding capacity. Although pure ingredients show higher functionalities, non-protein components could contribute to interfacial properties. Alkaline extraction, isoelectric precipitation and freeze-drying is the process mostly used in academic research to obtain PP ingredients. However, other extraction, purification, and drying methods can be properly combined, resulting in specific PP ingredient functionalities. Overall, this review highlights that, besides protein purity and source, knowledge of the processing history is required to select PP ingredients with desired functionalities.http://www.sciencedirect.com/science/article/pii/S2665927125000024Protein-rich plantsFunctional propertiesProtein extractionProtein purificationProtein flourProtein concentrate
spellingShingle Lorenzo Barozzi
Stella Plazzotta
Ada Nucci
Lara Manzocco
Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
Current Research in Food Science
Protein-rich plants
Functional properties
Protein extraction
Protein purification
Protein flour
Protein concentrate
title Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
title_full Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
title_fullStr Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
title_full_unstemmed Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
title_short Elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
title_sort elucidating the role of compositional and processing variables in tailoring the technological functionalities of plant protein ingredients
topic Protein-rich plants
Functional properties
Protein extraction
Protein purification
Protein flour
Protein concentrate
url http://www.sciencedirect.com/science/article/pii/S2665927125000024
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AT stellaplazzotta elucidatingtheroleofcompositionalandprocessingvariablesintailoringthetechnologicalfunctionalitiesofplantproteiningredients
AT adanucci elucidatingtheroleofcompositionalandprocessingvariablesintailoringthetechnologicalfunctionalitiesofplantproteiningredients
AT laramanzocco elucidatingtheroleofcompositionalandprocessingvariablesintailoringthetechnologicalfunctionalitiesofplantproteiningredients