Development of Transgenic Papaya through Agrobacterium-Mediated Transformation
Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the repo...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2013/235487 |
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| author | Md. Abul Kalam Azad Md. Golam Rabbani Latifah Amin Nik Marzuki Sidik |
| author_facet | Md. Abul Kalam Azad Md. Golam Rabbani Latifah Amin Nik Marzuki Sidik |
| author_sort | Md. Abul Kalam Azad |
| collection | DOAJ |
| description | Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the reporter gene. The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime for one week. The cocultivated explants were transferred into the final selection medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime + 50 mg/L kanamycin for callus induction as well as plant regeneration. The callus derived from the hypocotyls of Carica papaya cv. Shahi showed the highest positive GUS activities compared to Carica papaya cv. Ranchi. The transformed callus grew vigorously and formed embryos followed by transgenic plantlets successfully. The result of this study showed that the hypocotyls of C. papaya cv. Shahi and C. papaya cv. Ranchi are better explants for genetic transformation compared to immature embryos. The transformed C. papaya cv. Shahi also showed the maximum number of plant regeneration compared to that of C. papaya cv. Ranchi. |
| format | Article |
| id | doaj-art-2a76c7085a1d436f97352b2655622a0a |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-2a76c7085a1d436f97352b2655622a0a2025-02-03T01:31:10ZengWileyInternational Journal of Genomics2314-436X2314-43782013-01-01201310.1155/2013/235487235487Development of Transgenic Papaya through Agrobacterium-Mediated TransformationMd. Abul Kalam Azad0Md. Golam Rabbani1Latifah Amin2Nik Marzuki Sidik3Centre for General Studies, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MalaysiaDepartment of Horticulture, Bangladesh Agricultural University, Mymensingh 2202, BangladeshCentre for General Studies, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MalaysiaFaculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MalaysiaTransgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the reporter gene. The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime for one week. The cocultivated explants were transferred into the final selection medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime + 50 mg/L kanamycin for callus induction as well as plant regeneration. The callus derived from the hypocotyls of Carica papaya cv. Shahi showed the highest positive GUS activities compared to Carica papaya cv. Ranchi. The transformed callus grew vigorously and formed embryos followed by transgenic plantlets successfully. The result of this study showed that the hypocotyls of C. papaya cv. Shahi and C. papaya cv. Ranchi are better explants for genetic transformation compared to immature embryos. The transformed C. papaya cv. Shahi also showed the maximum number of plant regeneration compared to that of C. papaya cv. Ranchi.http://dx.doi.org/10.1155/2013/235487 |
| spellingShingle | Md. Abul Kalam Azad Md. Golam Rabbani Latifah Amin Nik Marzuki Sidik Development of Transgenic Papaya through Agrobacterium-Mediated Transformation International Journal of Genomics |
| title | Development of Transgenic Papaya through Agrobacterium-Mediated Transformation |
| title_full | Development of Transgenic Papaya through Agrobacterium-Mediated Transformation |
| title_fullStr | Development of Transgenic Papaya through Agrobacterium-Mediated Transformation |
| title_full_unstemmed | Development of Transgenic Papaya through Agrobacterium-Mediated Transformation |
| title_short | Development of Transgenic Papaya through Agrobacterium-Mediated Transformation |
| title_sort | development of transgenic papaya through agrobacterium mediated transformation |
| url | http://dx.doi.org/10.1155/2013/235487 |
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