Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System)
The reliability and availability of FOODLABfat system for determining acid value (AV) and peroxide value (POV) were assessed during the hydrolytic rancidification and lipid oxidation of edible oils. This reagent kit based on spectrophotometry was compared to the official methods (ISO 660 and 3960 pr...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/1468743 |
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| author | Chang Woo Kwon Kyung-Min Park Jeong Woong Park JaeHwan Lee Seung Jun Choi Pahn-Shick Chang |
| author_facet | Chang Woo Kwon Kyung-Min Park Jeong Woong Park JaeHwan Lee Seung Jun Choi Pahn-Shick Chang |
| author_sort | Chang Woo Kwon |
| collection | DOAJ |
| description | The reliability and availability of FOODLABfat system for determining acid value (AV) and peroxide value (POV) were assessed during the hydrolytic rancidification and lipid oxidation of edible oils. This reagent kit based on spectrophotometry was compared to the official methods (ISO 660 and 3960 protocols) based on manual titration employing the standard mixture for the simulated oxidation models and edible oils during the thermally induced oxidation at 180°C. The linear regression line of standard mixture and the significant difference of thermally oxidized time course study determined between them showed high correlations (R2=0.998 and p<0.05) in both AVs and POVs. Considering ISO protocols with a probability of human error in manual titration, the rapidness and simplicity of the reagent kit based on spectrophotometry make it a promising alternative to monitor the lipid oxidation of edible oils and lipid-containing foods. |
| format | Article |
| id | doaj-art-2a606e261c844bd8a46a88d81f1fd408 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-2a606e261c844bd8a46a88d81f1fd4082025-02-03T01:32:14ZengWileyJournal of Chemistry2090-90632090-90712016-01-01201610.1155/2016/14687431468743Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System)Chang Woo Kwon0Kyung-Min Park1Jeong Woong Park2JaeHwan Lee3Seung Jun Choi4Pahn-Shick Chang5Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Technology and Research, Sanigen Co., Ltd., Gwacheon 427-070, Republic of KoreaDepartment of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Republic of KoreaDepartment of Food Science and Technology and Department of Interdisciplinary Bio IT Materials, Seoul National University of Science and Technology, Seoul 139-743, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of KoreaThe reliability and availability of FOODLABfat system for determining acid value (AV) and peroxide value (POV) were assessed during the hydrolytic rancidification and lipid oxidation of edible oils. This reagent kit based on spectrophotometry was compared to the official methods (ISO 660 and 3960 protocols) based on manual titration employing the standard mixture for the simulated oxidation models and edible oils during the thermally induced oxidation at 180°C. The linear regression line of standard mixture and the significant difference of thermally oxidized time course study determined between them showed high correlations (R2=0.998 and p<0.05) in both AVs and POVs. Considering ISO protocols with a probability of human error in manual titration, the rapidness and simplicity of the reagent kit based on spectrophotometry make it a promising alternative to monitor the lipid oxidation of edible oils and lipid-containing foods.http://dx.doi.org/10.1155/2016/1468743 |
| spellingShingle | Chang Woo Kwon Kyung-Min Park Jeong Woong Park JaeHwan Lee Seung Jun Choi Pahn-Shick Chang Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System) Journal of Chemistry |
| title | Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System) |
| title_full | Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System) |
| title_fullStr | Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System) |
| title_full_unstemmed | Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System) |
| title_short | Rapid and Sensitive Determination of Lipid Oxidation Using the Reagent Kit Based on Spectrophotometry (FOODLABfat System) |
| title_sort | rapid and sensitive determination of lipid oxidation using the reagent kit based on spectrophotometry foodlabfat system |
| url | http://dx.doi.org/10.1155/2016/1468743 |
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